Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro

Yang, Xiao-Ning; Lu, Yu‐Chuan; Liu, Jingjing; Huang, Jiankun; Liu, Yunpeng; Xiao, Chuanxing; Jazag, Amarsanaa; Ren, Jianlin; Guleng, Bayasi

doi:10.1007/s10620-014-3044-3

Cited by 90 publications

(97 citation statements)

References 25 publications

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“…Because it is down-regulated in many small cell lung cancer cell lines, loss of Piezo1 has been suggested to play a role in increased cell migration and distant metastases in lung cancer (50). In contrast, knockdown of Piezo1 in gastric tumor cell lines was associated with decreased cell migration (51). These studies, along with those from endothelial cells, implicate a role for Piezo proteins in cell migration, proliferation, and elongation.…”

Section: Role Of Piezo1 In Cell Migration Proliferation and Elongationmentioning

confidence: 90%

Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Bagriantsev

Gracheva

Gallagher

2014

Journal of Biological Chemistry

197

174

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Section: Role Of Piezo1 In Cell Migration Proliferation and Elongationmentioning

confidence: 90%

Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Bagriantsev

Gracheva

Gallagher

2014

Journal of Biological Chemistry

197

174

View full text Add to dashboard Cite

“…cell migration and indicates that this interaction may be a therapeutic target in the invasion and metastasis of GC [31,118]. Interestingly, in normal lung epithelial cells, depletion of Piezo1 increases cell migration [118].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Page 5 enhancement of cell migration by TFF1 could provide an explanation for the apparent conflict that TFF1 expression is beneficial in normal tissues but detrimental in tumors [31]. In addition, TFF1 is involved in protecting the gastrointestinal mucosa from damage and repairing it through cell migration after injury [32,33].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

“…Piezo1 (also called FAM38A, Homo sapiens family with sequence similarity 38, member A), is a multi-pass transmembrane protein that is located in the endoplasmic reticulum [31]. Yang et al [31] reported that TFF1, but not TFF2 or TFF3, co-expressed with Piezo1 in the cytoplasm and interacted with the C-terminal region of…”

Section: Piezo1 and Cumentioning

confidence: 99%

“…Yang et al [31] reported that TFF1, but not TFF2 or TFF3, co-expressed with Piezo1 in the cytoplasm and interacted with the C-terminal region of…”

Section: Piezo1 and Cumentioning

confidence: 99%

See 2 more Smart Citations

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Xiao

Ling

Lan

et al. 2015

Clinica Chimica Acta

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Activation of Piezo1 contributes to matrix stiffness‐induced angiogenesis in hepatocellular carcinoma

Zhang

Wang

et al. 2022

Cancer Communications

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Background Despite integrin being highlighted as a stiffness‐sensor molecule in matrix stiffness‐driven angiogenesis, other stiffness‐sensor molecules and their mechanosensory pathways related to angiogenesis in hepatocellular carcinoma (HCC) remain obscure. Here, we explored the interplay between Piezo1 and integrin β1 in the mechanosensory pathway and their effects on HCC angiogenesis to better understand matrix stiffness‐induced angiogenesis. Methods The role of Piezo1 in matrix stiffness‐induced angiogenesis was investigated using orthotopic liver cancer SD rat models with high liver stiffness background, and its clinical significance was evaluated in human HCC tissues. Matrix stiffness‐mediated Piezo1 upregulation and activation were assayed using an in vitro fibronectin (FN)‐coated cell culture system with different stiffness, Western blotting and Ca2+ probe. The effects of shPiezo1‐conditioned medium (CM) on angiogenesis were examined by tube formation assay, wound healing assay and angiogenesis array. The underlying mechanism by which Piezo1 participated in matrix stiffness‐induced angiogenesis was analyzed by microRNA quantitative real‐time polymerase chain reaction (qRT‐PCR), matrix stiffness measurement, dual‐luciferase reporter assay, ubiquitination assay and co‐immunoprecipitation. Results Increased matrix stiffness significantly upregulated Piezo1 expression at both cellular and tissue levels, and high expression of Piezo1 indicated an unfavorable prognosis. High matrix stiffness also noticeably enhanced the activation level of Piezo1, similar to its expression level. Piezo1 knockdown significantly suppressed tumor growth, angiogenesis, and lung metastasis of HCC rat models with high liver stiffness background. shPiezo1‐CM from HCC cells attenuated tube formation and migration abilities of vascular endothelial cells remarkably, and analysis of differentially expressed pro‐angiogenic factors revealed that Piezo1 promoted the expression and secretion of vascular endothelial growth factor (VEGF), CXC chemokine ligand 16 (CXCL16) and insulin‐like growth factor binding protein 2 (IGFBP2). Matrix stiffness‐caused Piezo1 upregulation/activation restrained hypoxia inducible factor‐1α (HIF‐1α) ubiquitination, subsequently enhanced the expression of downstream pro‐angiogenic factors to accelerate HCC angiogenesis. Besides, collagen 1 (COL1)‐reinforced tissue stiffening resulted in more expression of Piezo1 via miR‐625‐5p. Conclusions This study unravels a new mechanism by which the integrin β1/Piezo1 activation/Ca2+ influx/HIF‐1α ubiquitination/VEGF, CXCL16 and IGFBP2 pathway participates in matrix stiffness‐driven HCC angiogenesis. Simultaneously, a positive feedback regulation loop as stiff matrix/integrin β1/miR‐625‐5p/Piezo1 and COL1/stiffer matrix mediates matrix stiffness‐caused Piezo1 upregulation.

show abstract

Piezo1 Is as a Novel Trefoil Factor Family 1 Binding Protein that Promotes Gastric Cancer Cell Mobility In Vitro

Abstract: Our data demonstrate that Piezo1 is a novel TFF1 binding protein that is important for TFF1-mediated cell migration and suggest that this interaction may be a therapeutic target in the invasion and metastasis of gastric cancer.

Cited by 90 publications

References 25 publications

Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Piezo Proteins: Regulators of Mechanosensation and Other Cellular Processes

Trefoil factors: Gastrointestinal-specific proteins associated with gastric cancer

Activation of Piezo1 contributes to matrix stiffness‐induced angiogenesis in hepatocellular carcinoma

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