PIF4-PAP1 interaction affects MYB-bHLH-WD40 complex formation and anthocyanin accumulation in Arabidopsis

Qin, Juan; Zhao, Chenbo; Wang, Shengwang; Gao, Na; Wang, Xiangxiang; Na, Xiaofan; Wang, Xiaomin; Bi, Yurong

doi:10.1016/j.jplph.2021.153558

Cited by 38 publications

(22 citation statements)

References 59 publications

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“…Recently, more attention has been given to the cultivation of ornamental plants with colored leaves; a phenotype that is mainly obtained by the accumulation of anthocyanins (Lightbourn et al, 2008 ; Li et al, 2021 ; Huang et al, 2022 ). Multiple R2R3-MYB and bHLH TFs have been reported as key regulators of anthocyanin biosynthesis (Allan et al, 2008 ; Petroni and Tonelli, 2011 ; Qin et al, 2022 ). A. commutatum is an excellent foliage plant with abundant leaf coloration; therefore, acquiring increasing importance for breeding desirable leaf color traits.…”

Section: Discussionmentioning

confidence: 99%

AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis in Aglaonema commutatum

et al. 2022

Front. Plant Sci.

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Aglaonema commutatum is one of the most popular foliage plants with abundant leaf phenotypes; therefore, anthocyanin coloration is a vital economic trait in A. commutatum. However, the molecular mechanisms underlying anthocyanin biosynthesis and its regulation remain unclear. In this study, AcMYB1 and AcbHLH1, transcription factor genes related to an R2R3-myeloblast (MYB) and a basic helix–loop–helix (bHLH), respectively, were isolated from A. commutatum “Red Valentine” and functionally characterized. AcMYB1 and AcbHLH1 were found to interact by Y2H and BiFC assay. AcMYB1 was grouped into the AN2 subgroup and shared high homology with the known regulators of anthocyanin biosynthesis. Gene expression analysis showed that both AcMYB1 and AcbHLH1 have similar expression patterns to anthocyanin structural genes and correlate with anthocyanin distribution in different tissues of A. commutatum. Light strongly promoted anthocyanin accumulation by upregulating the expression of anthocyanin-related genes in A. commutatum leaves. Ectopic expression of AcMYB1 in tobacco remarkably increased anthocyanin accumulation in both vegetative and reproductive tissues at various developmental stages. These results provide insights into the regulation of anthocyanin biosynthesis in A. commutatum and are useful for breeding new A. commutatum cultivars with enhanced ornamental value.

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Section: Discussionmentioning

confidence: 99%

AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis in Aglaonema commutatum

et al. 2022

Front. Plant Sci.

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“…The increased COP1 activity in high temperature is associated with the positive regulatory role of SUMO E3 ligase SIZ1, which also affects the expression of a substantial subset of PIF4-regulated genes ( Hammoudi et al, 2018 ). In addition, high temperature induces AtPIF4 expression and stabilizes AtPIF4, which inhibits activity of the MBW complex by targeting AtPAP1 at the transcriptional and protein levels ( Proveniers and van Zanten, 2013 ; Liu Z. et al, 2021 ; Qin et al, 2022 ). In apple, two heat shock transcription factors (HSFs) MdHSF3b and MdHSF4a activate MdCOL4 expression at high temperature, causing the inactivation of MdHY5 through the formation of the MdHY5-MdCOL4 complex ( Fang et al, 2019a ).…”

Section: R2r3-myb Is the Central Hub To Integrate Different Signaling...mentioning

confidence: 99%

“…Two ethylene response factors (ERF), Pp4ERF24 and Pp12ERF96, promote red Also, many anthocyanin repressors interfere with anthocyanin biosynthesis by interacting with R2R3-MYB transcriptional activators, disrupting the formation of MBW complex or changing the epigenetic status of downstream biosynthetic genes (Figure 3). In Arabidopsis, the miR156-targeted SQUAMOSA PROMOTER BINDING PROTEIN-LIKE9 (SPL9), jasmonate-ZIM-domain proteins (JAZs), and PIF4 are found to directly interact with AtPAP1, impairing the transcriptional activity of the MBW complex (Gou et al, 2011;Qi et al, 2011;Qin et al, 2022). A class II HD-Zip protein AtHAT1 recruited the TOPLESS corepressor via its EAR motifs in the N terminal, which forms a transcriptional repressor complex with AtPAP1 to destabilize the MBW complex and suppress histone H3 acetylation of ABG promoters (Zheng et al, 2019).…”

Section: Interaction With Coactivators or Competitorsmentioning

confidence: 99%

Multilevel regulation of anthocyanin-promoting R2R3-MYB transcription factors in plants

Yang

Chen²,

Zhang³

et al. 2022

Front. Plant Sci.

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Anthocyanins are common secondary metabolites in plants that confer red, blue, and purple colorations in plants and are highly desired by consumers for their visual appearance and nutritional quality. In the last two decades, the anthocyanin biosynthetic pathway and transcriptional regulation of anthocyanin biosynthetic genes (ABGs) have been well characterized in many plants. From numerous studies on model plants and horticultural crops, many signaling regulators have been found to control anthocyanin accumulation via regulation of anthocyanin-promoting R2R3-MYB transcription factors (so-called R2R3-MYB activators). The regulatory mechanism of R2R3-MYB activators is mediated by multiple environmental factors (e.g., light, temperature) and internal signals (e.g., sugar, ethylene, and JA) in complicated interactions at multiple levels. Here, we summarize the transcriptional control of R2R3-MYB activators as a result of natural variations in the promoter of their encoding genes, upstream transcription factors and epigenetics, and posttranslational modifications of R2R3-MYB that determine color variations of horticultural plants. In addition, we focus on progress in elucidating the integrated regulatory network of anthocyanin biosynthesis mediated by R2R3-MYB activators in response to multiple signals. We also highlight a few gene cascade modules involved in the regulation of anthocyanin-related R2R3-MYB to provide insights into anthocyanin production in horticultural plants.

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“…Anthocyanins are biosynthesized from phenylpropanoid and flavonoid pathways in the cytoplasm and transported into vacuoles [ 1 ]. The R2R3 MYB genes, bHLH transcription factors, and WD40 proteins are major regulators that play a central role in anthocyanins accumulation in plants [ 13 , 14 ]. These transcription factors collectively or independently regulate the downstream anthocyanin biosynthesis (enzymes) genes to accumulate anthocyanins in plants cells.…”

Section: Introductionmentioning

confidence: 99%

Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

Rao

Duan

Yang

et al. 2021

IJMS

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Saccharum officinarum (sugarcane) is the fifth major cultivated crop around the world. Sugarcane rind is a promising source for anthocyanin pigments; however, limited information is available on the anthocyanin and its biosynthesis in sugarcane rinds. In this study, we have quantified 49 compounds including 6 flavonoids and 43 anthocyanins in the rind of 6 sugarcane cultivars by using LCMS/MS approach. Thirty of them were quantified for the first time in sugarcane. The 43 anthocyanins included 10 cyanidin (Cya), 11 pelargonidin (Pel), 9 peonidin (Peo), 5 malvidin (Mal), 4 delphinidin (Del), and 4 petunidin (Pet) metabolites. High contents of Cya derivatives were observed in the rind of YT71/210 (dark purple rind), such as cya-3-O-(6-O-malonyl)-glu 1283.3 µg/g and cya-3-O-glu 482.67 µg/g followed by ROC22 (red rind) 821.3 µg/g and 409 µg/g, respectively, whereas the YT93/159 (green rind) showed a minimum level of these compounds. Among six cultivars, ROC22 rind has high levels of Peo derivatives such as peo-3-O-glu (197 µg/g), peo-3-O-(6-O-malonyl)-glu (69 µg/g) and peo-3-O-(6-O-p-coumaryl)-glu (55.17 µg/g). The gene expression analysis revealed that some genes, including a MYB(t) gene, were highly associated with the color phenotype. Thus, we cloned and overexpressed the gene in Arabidopsis and found the pinkish brown color in the hypocotyl of all transgenic lines compared with the wild type. Hence, we have quantified a wide range of anthocyanins in major sugarcane cultivars, reported many new anthocyanins for the first time, and concluded that Cya and Peo derivatives are the major contributing factor of dissimilar colors in sugarcane. The finding and the verification of a novel MYB gene involved in anthocyanin biosynthesis have demonstrated that our study was very valuable for gene discovery and genetic improvement of sugarcane cultivars to harvest high anthocyanin contents.

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PIF4-PAP1 interaction affects MYB-bHLH-WD40 complex formation and anthocyanin accumulation in Arabidopsis

Cited by 38 publications

References 59 publications

AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis in Aglaonema commutatum

AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis in Aglaonema commutatum

Multilevel regulation of anthocyanin-promoting R2R3-MYB transcription factors in plants

Novel Insights into Anthocyanin Metabolism and Molecular Characterization of Associated Genes in Sugarcane Rinds Using the Metabolome and Transcriptome

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