Pigment production by Scenedesmus dimorphus using different low‐cost and alternative culture media

Abstract

BACKGROUND: This study investigated the feasibility of producing pigments from Scenedesmus dimorphus using different lowcost synthetic and alternative media. Thus the influence of nitrogen and phosphorus deficiency, different nitrogen sources and alternative culture media (wheat bran, garlic powder, wheat bran supplemented with garlic powder) on pigment and biomass production was evaluated. RESULTS: Among the three sources of nitrogen studied (ammonium sulfate, urea and sodium nitrate), urea was the cheapest efficient alternative source of nitrogen for pigment production, especially total chlorophylls (67.38 ∼g L −1 d −1 ), by this strain, without strongly affecting biomass yield. Phosphorus deficiency had no negative effect on biomass production, but improved the production of zeaxanthin and chlorophyll b by13.1% and 26.8%, respectively, compared to control. Compared to BG-11 medium, the alternative garlic powder medium increased the biomass yield of S. dimorphus by 5.2 times, improved the productivity of xanthophylls and contributed to the accumulation of ⊎-carotene. Wheat bran medium was found to be efficient as a zero-cost culture medium for the production of pigments, where S. dimorphus accumulated twice the total pigment concentration of the control, with high ⊎-carotene productivity (38.58 ± 11.94 ∼g L −1 d −1 ). The addition of garlic powder to wheat bran increased the biomass (3.14 g L −1 ) and pigment productivity (216.86 ± 2.06 ∼g L −1 d −1 ) by 28.5 and 1.4 times, respectively, but inhibited zeaxanthin biosynthesis. This alternative medium reduced biomass and pigment production costs by 99.80% and 96.21%, respectively. CONCLUSION: Wheat bran supplemented with garlic powder showed good application potential for the industrial production of biomass and pigments from S. dimorphus in open cultivation systems where garlic powder might be used simultaneously for biomass and pigment accumulation, and for biological contamination control.

Comparison of Light Intensity Effect On Microalgal Growth in Cactus-like and Cylindrical Photo Bioreactors

“…The microalgae strains were cultivated in a 2.7 L BG11 medium. 0.3 L of pre-culture was inoculated into the PBR [20]. The room temperature where the microalgae were grown was kept at 25 °C.…”

Chromatographic Techniques to Separate and Identify Bioactive Compounds in Microalgae

“…dimethyl formamide, sec-butanol, methanol) that allows a rapid and efficient extraction from microalgae with a good stability of pigments (Schumann, Häubner, Klausch, & Karsten, 2005). A range of extraction techniques have been studied to maximize chlorophylls extraction, including MAE extraction for the extraction of chlorophyll a and b in Dunaliella tertiolecta and Cylindrotheca closterium (Ahmad et al, 2022).…”

“…can be employed as mobile phase. Elution gradients (instead of isocratic operation) are preferred for more accurate results (Maroneze et al, 2019;Fernandes et al, 2020;Ahmad et al, 2022). In a recent research, Fernandes et al, revealed that HPLC-PDA with a C30 column can successfully separate different type of chlorophylls when a mixture of methanol:methyl tert-butyl ether:water is used as the mobile phase with a changing gradient (Fernandes et al, 2020).…”

Design and Deconstruction of Chemical Process Flow Based on Deep Learning

“…e chemical process design is also mainly divided into two stages [14]. e first stage is preliminary preparation.…”