Pilot evaluation of a single oral fecal microbiota transplantation for canine atopic dermatitis

Sugita, Koji; Shima, Ayaka; Takahashi, Kaho; Ishihara, Genki; Kawano, Koji; Ohmori, Kitaro

doi:10.1038/s41598-023-35565-y

Cited by 12 publications

(13 citation statements)

References 45 publications

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“…This observation is in line with previous studies [ 52 ]. When comparing the gut microbiota in healthy dogs with that in cAD-affected dogs (cADpre), the abundance of Fusobacteria was significantly reduced in cAD-affected dogs, which is in line with previous studies of gut microbiota in dogs affected with cAD and healthy dogs [ 22 , 23 ]. This was further illustrated at the genus level, presenting the reduction of Fusobacterium abundance in cADpre dogs compared with that in healthy dogs.…”

Section: Discussionsupporting

confidence: 89%

“…This is considered to be a similar situation for the clinical management of cAD. While several studies of gut microbiota in cAD-affected dogs have recently been reported; a pilot study using a limited number of Beagles [ 21 ] and one breeding colony of Shiba Inu dogs [ 22 ], or a study of multiple dog breeds [ 23 ], no studies dissecting both skin gut microbiota in cAD-affected dogs are available to date.…”

Section: Introductionmentioning

confidence: 99%

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A comprehensive analysis of gut and skin microbiota in canine atopic dermatitis in Shiba Inu dogs

Thomsen,

Künstner,

Wohlers

et al. 2023

Microbiome

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Background Like its human counterpart, canine atopic dermatitis (cAD) is a chronic relapsing condition; thus, most cAD-affected dogs will require lifelong treatment to maintain an acceptable quality of life. A potential intervention is modulation of the composition of gut microbiota, and in fact, probiotic treatment has been proposed and tried in human atopic dermatitis (AD) patients. Since dogs are currently receiving intensive medical care, this will be the same option for dogs, while evidence of gut dysbiosis in cAD is still missing, although skin microbial profiling in cAD has been conducted in several studies. Therefore, we conducted a comprehensive analysis of both gut and skin microbiota in cAD in one specific cAD-predisposed breed, Shiba Inu. Additionally, we evaluated the impact of commonly used medical management on cAD (Janus kinase; JAK inhibitor, oclacitinib) on the gut and skin microbiota. Furthermore, we genotyped the Shiba Inu dogs according to the mitochondrial DNA haplogroup and assessed its association with the composition of the gut microbiota. Results Staphylococcus was the most predominant bacterial genus observed in the skin; Escherichia/Shigella and Clostridium sensu stricto were highly abundant in the gut of cAD-affected dogs. In the gut microbiota, Fusobacteria and Megamonas were highly abundant in healthy dogs but significantly reduced in cAD-affected dogs. The abundance of these bacterial taxa was positively correlated with the effect of the treatment and state of the disease. Oclacitinib treatment on cAD-affected dogs shifted the composition of microbiota towards that in healthy dogs, and the latter brought it much closer to healthy microbiota, particularly in the gut. Additionally, even within the same dog breed, the mtDNA haplogroup varied, and there was an association between the mtDNA haplogroup and microbial composition in the gut and skin. Conclusions Dysbiosis of both the skin and the gut was observed in cAD in Shiba Inu dogs. Our findings provide a basis for the potential treatment of cAD by manipulating the gut microbiota as well as the skin microbiota.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

A comprehensive analysis of gut and skin microbiota in canine atopic dermatitis in Shiba Inu dogs

Thomsen,

Künstner,

Wohlers

et al. 2023

Microbiome

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“…Sugita et al. ( 2023 ) reported that the gut microbiota plays a pivotal role in the pathogenesis of canine atopic dermatitis and that oral faecal microbiota transplantation may be a novel therapeutic approach to target the gut microbiota in canine atopic dermatitis. Similarly, K. Ural ( 2022 ) reported that faecal microbiota transplantation capsules were beneficial in the treatment of canine atopic dermatitis.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the relationship between atopic dermatitis of dogs and intestinal epithelial damage

Ekici,

2024

Veterinary Medicine & Sci

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BackgroundA significant association between atopic dermatitis and leaky gut syndrome has been demonstrated in humans. No studies have been conducted to determine whether there is an association between atopic dermatitis and intestinal damage in dogs.ObjectivesThis study aimed to determine whether there is an association between canine atopic dermatitis and intestinal damage using selected intestinal‐related biomarkers.MethodsTwenty‐six dogs with atopic dermatitis and 10 healthy dogs were included. Moderate‐to‐severe pruritus, erythema, erosion and alopecia on different parts of the body were sought in dogs to suspect atopic dermatitis. The presence of atopic dermatitis was confirmed by an allergic skin test. Serum biomarkers including intestinal fatty acid binding protein (I‐FABP), intestinal alkaline phosphatase (IAP), trefoil factor‐3 (TFF‐3), immunoglobulin E (IgE), interleukin‐4 (IL‐4) and interleukin‐13 (IL‐13) concentrations were measured from venous blood samples.ResultsOf the 26 dogs tested for allergens, 16 were found to be sensitive to mould mites, 10 to vernal grass, eight to house dust mites, five to wheat dust and five to grass pollen mix allergens. Significant increases in serum IAP, TFF‐3, IgE, IL‐4 and IL‐13 concentrations were determined.ConclusionIt was thought that the increase in TFF‐3 and IAP concentrations may be due to the presence of intestinal epithelial damage and the repair of this damage. In addition, the development of atopic dermatitis may be predisposed to the entry of allergens into the body through sites of intestinal damage.

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“…A systematic review demonstrated that while nearly half of included studies reported a positive effect of an altered gut microbial colonization due to the use of probiotics on the severity of AD, other studies did not observe such effect ( 13 ). Recently, animal studies have found that FMT treatment benefits mice or dogs with AD by enhanccing gut microbiota homeostasis ( 7 , 14 – 16 ). In 2022, Mashiah et al.…”

Section: Discussionmentioning

confidence: 99%

Washed microbiota transplantation: a case report of clinical success with skin and gut microbiota improvement in an adolescent boy with atopic dermatitis

Deng,

Chen,

Zhong

et al. 2023

Front. Immunol.

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Atopic dermatitis (AD) is a chronic, recurrent inflammatory disease characterized by itching. The gut microbiome can help maintain skin immune homeostasis by regulating innate and adaptive immunity. Here, we report a case of AD in a 15-year-old adolescent boy who benefited from washed microbiota transplantation (WMT). WMT was performed for three courses, with each course lasting for three consecutive days and an interval of one month between two courses. Clinical assessments were conducted at each WMT course, and skin, blood, and stool samples were collected for microbial analysis. After three months of WMT treatment, the boy’s itchiness was effectively controlled: his skin showed noticeable improvement, with reduced Staphylococcus aureus in the skin lesions. The scores of SCORAD (SCORing Atopic Dermatitis), EASI (Eczema Area and Severity Index), NRS (Numerical Rating Scale), and DLQI (Dermatology Life Quality Index) significantly decreased compared to the baseline. Serum levels of eosinophil ratio, tumor necrotic factor-α, and interleukin-6 also reduced to the normal levels. There was a significant decrease in S. aureus in the skin lesions. Additionally, the intestinal flora became more diverse, and the abundance of Bifidobacterium species, significantly increased after WMT. No adverse events were reported during the treatment and the 1-year follow-up period. This case report provides direct clinical evidence for WMT as a novel promising treatment strategy for AD, and preliminary experimental data suggests the existence of an intestinal-skin axis in terms of the gut microbiota and the skin immune homeostasis.

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Pilot evaluation of a single oral fecal microbiota transplantation for canine atopic dermatitis

Cited by 12 publications

References 45 publications

A comprehensive analysis of gut and skin microbiota in canine atopic dermatitis in Shiba Inu dogs

A comprehensive analysis of gut and skin microbiota in canine atopic dermatitis in Shiba Inu dogs

Investigation of the relationship between atopic dermatitis of dogs and intestinal epithelial damage

Washed microbiota transplantation: a case report of clinical success with skin and gut microbiota improvement in an adolescent boy with atopic dermatitis

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