Pilot scale dilute acid pretreatment of rice straw and fermentable sugar recovery at high solid loadings

Kapoor, Manali; Soam, Shveta; Agrawal, Ruchi; Gupta, Ravi P.; Tuli, Deepak K.; Kumar, Ravindra

doi:10.1016/j.biortech.2016.11.032

Cited by 79 publications

(72 citation statements)

References 27 publications

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“…Penicillium sp. A pilot level dilute acid pretreatment of rice straw, followed by enzymatic saccharification at high solid loadings resulted in high sugar recovery [9]. Xylanase from Chaetomium globosum exhibited better performance for saccharification of rice straw than the commercial xylanase [6].…”

Section: Assessment Of a Terreus S9 Xylanase For Hydrolysis Of Rice mentioning

confidence: 99%

“…Being an heteropolysaccharide xylan requires several enzymes for its complete breakdown but endo b-(1,4)-xylanase or simply xylanase (EC 3.2.1.8), is the main enzyme which cleaves the internal b-(1,4)-linked Dxylosyl glycosidic bonds in heteroxylan [6,7]. Xylanases may have application potential for several biotechnological processes such as pulp and paper industries for biobleaching, nutrition-rich animal feed production, bioconversion of LB into simple sugars that can be fermented to produce bioethanol/butanol (biofuel), xylitol, and a variety of other platform chemicals/materials of commercial importance [3,8,9].…”

Section: Introductionmentioning

confidence: 99%

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Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach

Sharma

Bajaj

2017

Env Prog and Sustain Energy

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High production cost and lack of process‐suitable characteristics in available microbial xylanases limit their vast industrial application potential. A new strain of Aspergillus terreus S9 isolated from mushroom compost (Directorate of Mushroom Research, Solan, India), utilized agro‐industrial wastes as substrates for growth, and produced a process‐apt xylanase. The study is the first ever report of a thermostable (60–90°C) and broad range pH stable (6.0–10.0) xylanase from a strain of Aspergillus terreus. Of a total of ten process variables examined based on Plackett‐Burman design, four variables, i.e., wheat bran, incubation time, pH, and CaCl2 were earmarked to have substantial influence on xylanase production. These selected four variables were further optimized using Design of Experiments (DoE) approach (response surface methodology), and xylanase yield was enhanced (1.92‐fold). Saccharification potential of xylanase was assessed using rice straw as substrate, under several sets of combinatorial regimes. Results showed that alkali pretreatment (0.2M KOH) followed by acid pretreatment (1% sulphuric acid) and enzymatic hydrolysis with cellulase and xylanase yielded maximum sugars. Desired industrial traits of A. terreus S9 xylanase reflect its application prospective for valorization of lignocellulosic biomass for production of biofuel‐ethanol/other industrial products. © 2017 American Institute of Chemical Engineers Environ Prog, 37: 1210–1219, 2018

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Section: Assessment Of a Terreus S9 Xylanase For Hydrolysis Of Rice mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach

Sharma

Bajaj

2017

Env Prog and Sustain Energy

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“…Para la evaluación del pretratamiento se emplearon tres concentraciones de ácido sulfúrico diluido (0,5; 1; 1,5% p/v) [23,24,25] y tres concentraciones de sólidos (5; 8,5; 12% p/p) [26]. La biomasa lignocelulósica y el ácido diluido se colocaron por gravimetría en función a una base de cálculo de 100 g (ácido sulfúrico diluido + biomasa seca) en Cuadro 2.…”

Section: Pretratamiento Con áCido Diluidounclassified

Efecto del pretratamiento con ácido sulfúrico diluido sobre la hidrólisis enzimática del Panicum maximum

SALDARRIAGA¹,

LOZANO²

2018

BSAA

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“…Pretreatment of lignocellulosic biomass with dilute mineral acids under mild conditions has been found to effectively separate and break down hemicellulose, modify the lignin network and increase cellulose accessibility [8,9]. Overall, the technology of dilute-acid pretreatment is being actively studied and applied; in 2017 alone, a number of papers have been published in the field of dilute-acid pretreatment of biomass [10][11][12][13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Kinetic Modelling and Experimental Studies for the Effects of Fe2+ Ions on Xylan Hydrolysis with Dilute-Acid Pretreatment and Subsequent Enzymatic Hydrolysis

et al. 2018

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High-temperature (150-170 • C) pretreatment of lignocellulosic biomass with mineral acids is well established for xylan breakdown. Fe 2+ is known to be a cocatalyst of this process although kinetics of its action remains unknown. The present work addresses the effect of ferrous ion concentration on sugar yield and degradation product formation from corn stover for the entire two-step treatment, including the subsequent enzymatic cellulose hydrolysis. The feedstock was impregnated with 0.5% acid and 0.75 mM iron cocatalyst, which was found to be optimal in preliminary experiments. The detailed kinetic data of acid pretreatment, with and without iron, was satisfactorily modelled with a four-step linear sequence of first-order irreversible reactions accounting for the formation of xylooligomers, xylose and furfural as intermediates to provide the values of Arrhenius activation energy. Based on this kinetic modelling, Fe 2+ turned out to accelerate all four reactions, with a significant alteration of the last two steps, that is, xylose degradation. Consistent with this model, the greatest xylan conversion occurred at the highest severity tested under 170 • C/30 min with 0.75 mM Fe 2+ , with a total of 8% xylan remaining in the pretreated solids, whereas the operational conditions leading to the highest xylose monomer yield, 63%, were milder, 150 • C with 0.75 mM Fe 2+ for 20 min. Furthermore, the subsequent enzymatic hydrolysis with the prior addition of 0.75 mM of iron(II) increased the glucose production to 56.3% from 46.3% in the control (iron-free acid). The detailed analysis indicated that conducting the process at lower temperatures yet long residence times benefits the yield of sugars. The above kinetic modelling results of Fe 2+ accelerating all four reactions are in line with our previous mechanistic research showing that the pretreatment likely targets multiple chemistries in plant cell wall polymer networks, including those represented by the C-O-C and C-H bonds in cellulose, resulting in enhanced sugar solubilization and digestibility.

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Pilot scale dilute acid pretreatment of rice straw and fermentable sugar recovery at high solid loadings

Cited by 79 publications

References 27 publications

Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach

Xylanase production from a new strain of Aspergillus terreus S9 and its application for saccharification of rice straw using combinatorial approach

Efecto del pretratamiento con ácido sulfúrico diluido sobre la hidrólisis enzimática del Panicum maximum

Kinetic Modelling and Experimental Studies for the Effects of Fe2+ Ions on Xylan Hydrolysis with Dilute-Acid Pretreatment and Subsequent Enzymatic Hydrolysis

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