PIMS-TS immunophenotype: description and comparison with healthy children, Kawasaki disease and severe viral and bacterial infections

García-Salido, Alberto; Leoz-Gordillo, Inés; Brabin, Anthony González; Teresa, María; Martínez-de-Azagra-Garde, Amelia; Iglesias-Bouzas, María Isabel; Cabrero-Hernández, Marta; Caro-Patón, G. de Lama; Unzueta-Roch, José Luis; Castillo-Robleda, Ana; Ramírez-Orellana, Manuel; Nieto-Moro, Montserrat

doi:10.1080/23744235.2022.2059561

Cited by 3 publications

(5 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This molecule is constitutively expressed on monocytes; also, it shows low surface levels on neutrophils in the absence of inflammation. This molecule has been described as a useful clinical and inflammatory biomarker for SARS‐CoV‐2 infection 8,21 . We observed a higher percentage of CD64 + neutrophils in children with previous SARS‐CoV‐2 infection.…”

Section: Discussionmentioning

confidence: 53%

“…2,5 Its expression directly and indirectly indicates inflammatory status; therefore, its description after SARS-CoV-2 infection may be of interest. 7,8 Therefore, in this study, we examined IgG antispike persistence in a group of children infected with SARS-CoV-2 during the first two pandemic waves. Simultaneously, their immunophenotype was described through the study of leukocyte populations and CD64 expression.…”

Section: Introductionmentioning

confidence: 99%

“…This immunoglobulin is expressed on monocytes and neutrophils 1,3 and is upregulated by cytokine expression 2,5 . Its expression directly and indirectly indicates inflammatory status; therefore, its description after SARS‐CoV‐2 infection may be of interest 7,8 …”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

IgG antispike persistence and immunophenotype in children infected by SARS‐CoV‐2

et al. 2023

View full text Add to dashboard Cite

Aim:The immune status of children recovering from SARS-CoV-2 infection is not completely understood. We describe IgG antispike persistence in children infected during the first two pandemic waves. In addition, we compared with healthy controls their leukocyte populations and CD64 expression.Methods: Cross-sectional study. Carried out from October 2021 to February 2022 in nonreinfected and nonvaccinated children with SARS-CoV-2 in 2020. The presence of antispike IgG was studied using chemiluminescent immunoassay. Leukocyte populations were analysed using flow cytometry and marked for CD45, CD4, CD8 and CD64. Statistical minor than 0.05 was considered significant.Results: One hundred and eighty-three control and 77 patients were included. IgG antispike determinations were performed after a median of 501 days (262-464); 52 of 77 children were positive. Cases showed significantly higher percentages of monocytes, lymphocytes, CD8 + and CD4 + . In addition, CD64 expression was higher in monocytes and neutrophils. The presence of IgG antispike was accompanied by a higher percentage of CD64 + neutrophils. Conclusion:In our series, the SARS-CoV-2 IgG antispike protein was usually positive beyond 1 year after infection. Furthermore, leukocyte populations from cases differ from controls, with higher CD64 expression on neutrophils and monocytes.Prospective clinical observations are required to confirm the implications of these findings.

show abstract

Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

IgG antispike persistence and immunophenotype in children infected by SARS‐CoV‐2

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Our results contrast those of García-Salido et al . (2022), who reported high baseline expression of CD11b in monocytes and neutrophils in children with MIS-C compared to healthy controls and in children with KD 23 . These differences could be due to the number of patients included in the studies and the different methodological approaches employed when defining neutrophil and monocyte populations by flow cytometry.…”

Section: Discussionmentioning

confidence: 99%

“…These differences could be due to the number of patients included in the studies and the different methodological approaches employed when defining neutrophil and monocyte populations by flow cytometry. Furthermore, contradictory results indicating decreased or increased expression of CD11b in neutrophils in KD compared to controls could suggest heterogeneity in the expression of this marker in the population and the need for a larger cohort of studies 23 – 25 .…”

Section: Discussionmentioning

confidence: 99%

Innate immune dysregulation in multisystem inflammatory syndrome in children (MIS-C)

Isaza-Correa,

Ryan,

Kelly

et al. 2023

Sci Rep

View full text Add to dashboard Cite

MIS-C is a systemic inflammation disorder with poorly characterised immunopathological mechanisms. We compared changes in the systemic immune response in children with MIS-C (n = 12, 5–13 years) to healthy controls (n = 14, 5–15 years). Analysis was done in whole blood treated with LPS. Expression of CD11b and Toll-like receptor-4 (TLR4) in neutrophils and monocytes were analysed by flow cytometry. Serum cytokines (IL-1β, IL-2, IL-6, IL-8, IL-10, IL-Ira, TNF-α, TNF-β, IFN-Υ, VEGF, EPO and GM-CSF) and mRNA levels of inflammasome molecules (NLRP3, ASC and IL-1β) were evaluated. Subpopulations of lymphocytes (CD3+, CD19+, CD56+, CD4+, CD8+, TCR Vδ1+, TCR Vδ2+) were assessed at basal levels. Absolute counts of neutrophils and NLR were high in children with MIS-C while absolute counts of lymphocytes were low. Children with MIS-C had increased levels of IL-6, IL-10, TNF-β and VEGF serum cytokines at the basal level, and significantly increased TNF-β post-LPS, compared to controls. IL-1RA and EPO decreased at baseline and post-LPS in MIS-C patients compared to controls. The percentage of CD3+ cells, NK cells and Vδ1 was lower while B cells were higher in children with MIS-C than in controls. Dysregulated immune response in children with MIS-C was evident and may be amenable to immunomodulation.

show abstract

Leukocyte activation patterns in hospitalized children: comparing SARS-CoV-2, bacterial infections, and inflammatory pathologies

Domitien Payet,

Bedin,

Desselas

et al. 2024

Journal of Leukocyte Biology

View full text Add to dashboard Cite

In adults, monocytes and neutrophils play important roles in the hyper-inflammatory responses’ characteristic of severe forms of SARS-CoV-2 infection. We assessed leukocyte activation in 55 children attending the emergency department for acute fever between March 2020 and September 2021. The following markers were analyzed by flow cytometry: CD169 and HLA-DR on monocytes, CD64 and CD16 on neutrophils, CD38 on lymphocytes TCD8. Fifteen of the children had SARS-CoV-2 infection, 15 had bacterial infections, 15 had inflammatory diseases. We observed overexpression of CD169 on monocytes and CD38 on lymphocytes T in all patients with a diagnosis of SARS-CoV-2, while overexpression of CD64 on neutrophils was observed with bacterial infections and inflammatory diseases. There was a decrease in the expression of HLA-DR on monocytes in the bacterial infection and inflammatory pathology groups. Leukocyte analysis identifies distinct activation patterns in children during SARS-CoV-2 infections, bacterial infections, and inflammatory diseases.

show abstract

PIMS-TS immunophenotype: description and comparison with healthy children, Kawasaki disease and severe viral and bacterial infections

Cited by 3 publications

References 16 publications

IgG antispike persistence and immunophenotype in children infected by SARS‐CoV‐2

IgG antispike persistence and immunophenotype in children infected by SARS‐CoV‐2

Innate immune dysregulation in multisystem inflammatory syndrome in children (MIS-C)

Leukocyte activation patterns in hospitalized children: comparing SARS-CoV-2, bacterial infections, and inflammatory pathologies

Contact Info

Product

Resources

About