PIN Polar Targeting

Feraru, Elena; Friml, Jiřı́

doi:10.1104/pp.108.121756

Cited by 140 publications

(117 citation statements)

References 68 publications

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“…The asymmetric localization of auxin influx and efflux carrier proteins depends on the actin-dependent vesicle transport machinery (14,15). Both auxin efflux and influx components PIN-FORMED (PINs) proteins and AUXIN INFLUX1 (AUX1)/LIKE AUX1(LAXs) undergo constitutive cycling between the plasma membrane and endosomes (12,(16)(17)(18)(19). GNOM-dependent and independent pathways have been generally described based on the sensitivity to brefeldin A (BFA) treatment (20,21).…”

mentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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The vacuole is the most prominent compartment in plant cells and is important for ion and protein storage. In our effort to search for key regulators in the plant vacuole sorting pathway, ribosomal large subunit 4 (rpl4d) was identified as a translational mutant defective in both vacuole trafficking and normal development. Polysome profiling of the rpl4d mutant showed reduction in polysome-bound mRNA compared with wild-type, but no significant change in the general mRNA distribution pattern. Ribsomal profiling data indicated that genes in the lipid metabolism pathways were translationally down-regulated in the rpl4d mutant. Live imaging studies by Nile red staining suggested that both polar and nonpolar lipid accumulation was reduced in meristem tissues of rpl4d mutants. Pharmacological evidence showed that sterol and sphingolipid biosynthetic inhibitors can phenocopy the defects of the rpl4d mutant, including an altered vacuole trafficking pattern. Genetic evidence from lipid biosynthetic mutants indicates that alteration in the metabolism of either sterol or sphingolipid biosynthesis resulted in vacuole trafficking defects, similar to the rpl4d mutant. Tissue-specific complementation with key enzymes from lipid biosynthesis pathways can partially rescue both vacuole trafficking and auxin-related developmental defects in the rpl4d mutant. These results indicate that lipid metabolism modulates auxin-mediated tissue differentiation and endomembrane trafficking pathways downstream of ribosomal protein function.

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mentioning

confidence: 99%

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Sun

Hicks

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…3), suggesting that VIP1-mediated signaling is distinct from FERONIAmediated signaling. Because auxin is transported via the root cap, where many of the PIN members are expressed (for review, see Feraru and Friml, 2008), changes in the root cap structures can affect the auxin distribution and/or the local auxin responses around the root tip. Both PIN1 and PIN2 levels can be affected by local auxin levels (Abas et al, 2006;Omelyanchuk et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

Tsugama¹,

Liu²,

Takano³

2016

Plant Physiol.

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VIP1 is a bZIP transcription factor in Arabidopsis (Arabidopsis thaliana). VIP1 transiently accumulates in the nucleus when cells are exposed to hypoosmotic conditions, but its physiological relevance is unclear. This is possibly because Arabidopsis has approximately 10 close homologs of VIP1 and they function redundantly. To examine their physiological roles, transgenic plants overexpressing a repression domain-fused form of VIP1 (VIP1-SRDXox plants), in which the gene activation mediated by VIP1 is expected to be repressed, were generated. Because hypoosmotic stress can mimic mechanical stimuli (e.g. touch), the touchinduced root-waving phenotypes and gene expression patterns in those transgenic plants were examined. VIP1-SRDXox plants exhibited more severe root waving and lower expression of putative VIP1 target genes. The expression of the VIP1-green fluorescent protein (GFP) fusion protein partially suppressed the VIP1-SRDX-induced increase in root waving when expressed in the VIP1-SRDXox plants. These results suggest that VIP1 can suppress the touch-induced root waving. The VIP1-SRDX-induced increase in root waving was also suppressed when the synthetic auxin 2,4-dichlorophenoxy acetic acid or the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, which is known to activate auxin biosynthesis, was present in the growth medium. Root cap cells with the auxin marker DR5rev::GFP were more abundant in the VIP1-SRDXox background than in the wild-type background. Auxin is transported via the root cap, and the conditions of outermost root cap layers were abnormal in VIP1-SRDXox plants. These results raise the possibility that VIP1 influences structures of the root cap and thereby regulates the local auxin responses in roots.

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“…Relatively little is known about this regulation, but it is clear that auxin itself plays a central role. Auxin is known to regulate PINs at multiple stages, affecting the transcription of PIN genes (44) , the cycling of PIN proteins between the plasma membrane and endomembrane compartments (45)(46)(47)(48) , and the stability of PINs (49) . Since the amount of auxin both inside and outside cells is affected by PINs, auxins and their transporters are intimately linked in complex feedback loops.…”

Section: Auxin's Roles In Plant Developmentmentioning

confidence: 99%

Computer simulation: The imaginary friend of auxin transport biology

et al. 2010

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Regulated transport of the plant hormone auxin is central to many aspects of plant development. Directional transport, mediated by membrane transporters, produces patterns of auxin distribution in tissues that trigger developmental processes, such as vascular patterning or leaf formation. Experimentation has produced a lot of largely qualitative data providing strong evidence for multiple feedback systems between auxin and its transport. However the exact mechanisms concerned remain elusive and the experiments required to evaluate alternative hypotheses are challenging. Because of this, computational modelling now plays an important role in auxin transport research. Here we review current approaches and underlying assumptions of computational auxin transport models, including recent attempts to unify conflicting mechanistic explanations. In addition we discuss in general how computer simulation of these processes is proving to be an increasingly effective method of hypothesis generation and testing, and how simulation can be used to direct future experiments.

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PIN Polar Targeting

Cited by 140 publications

References 68 publications

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

Arabidopsis ribosomal proteins control vacuole trafficking and developmental programs through the regulation of lipid metabolism

The bZIP protein VIP1 is involved in touch responses in Arabidopsis roots

Computer simulation: The imaginary friend of auxin transport biology

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