PIN1P1 is activated by CREB1 and promotes gastric cancer progression via interacting with YBX1 and upregulating PIN1

Abstract

Long noncoding RNAs (lncRNAs) play critical roles in the carcinogenesis and progression of cancers. However, the role and mechanism of the pseudogene lncRNA PIN1P1 in gastric carcinoma remain unclear. The expression and effects of lncRNA PIN1P1 in gastric cancer were investigated. The transcriptional regulation of CREB1 on PIN1P1 was determined by ChIP and luciferase assays. The mechanistic model of PIN1P1 in gastric cancer was further explored by RNA pull‐down, RIP and western blot analysis. PIN1P1 was overexpressed in gastric cancer tissues, and upregulated PIN1P1 predicted poor prognosis in patients. CREB1 was directly combined with the promoter region of PIN1P1 to promote the transcription of PIN1P1. CREB1‐mediated enhanced proliferation, migration and invasion could be partially reversed by downregulation of PIN1P1. Overexpressed PIN1P1 promoted the proliferation, migration and invasion of gastric cancer cells, whereas decreased PIN1P1 showed the opposite effects. PIN1P1 directly interacted with YBX1 and promoted YBX1 protein expression, leading to upregulation of PIN1, in which E2F1 may be involved. Silencing of YBX1 during PIN1P1 overexpression could partially rescue PIN1 upregulation. PIN1, the parental gene of PIN1P1, was elevated in gastric cancer tissues, and its upregulation was correlated with poor patient outcomes. PIN1 facilitated gastric cancer cell proliferation, migration and invasion. To sum up, CREB1‐activated PIN1P1 could promote gastric cancer progression through YBX1 and upregulating PIN1, suggesting that it is a potential target for gastric cancer.

Carvacrol induces osteogenic differentiation of BMSCs and alleviates osteolysis in aged mice by upregulating lncRNA NEAT1 to promote SIRT1 expression

“…Our results also show that inhibition of NEAT1 attenuates the protective effect of carvacrol on calvarial bone and is involved in osteolysis. LncRNAs can be involved in the regulation of gene expression through various mechanisms [65,66], including the regulation of SIRT1 through ubiquitination [67,46,47]. To further investigate the relationship between NEAT1 and SIRT1, we performed RNA pull-down and RIP experiments.…”

Y-Box Binding Protein 1: Unraveling the Multifaceted Role in Cancer Development and Therapeutic Potential

“…This phosphorylation is crucial for the nuclear translocation of YBX1 and its involvement in apoptosis surveillance in glioma stem cells [74]. CREB1-activated PIN1P1 contributes to gastric cancer progression by interacting with YBX1 and inducing PIN1 upregulation [75]. Oncogenic AURKA influences breast cancer-related RNA splicing, interacting with YBX1 to promote GOLGA4 exon inclusion and with hnRNPK to induce RBM4 exon skipping [76].…”