Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition

Zhang, Zecai; Cao, Hongshi; Shen, Peng; Liu, Jiuxi; Cao, Yongguo; Zhang, Naisheng

doi:10.1016/j.jep.2020.112715

Cited by 25 publications

(18 citation statements)

References 27 publications

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“…Rhein, the active ingredient of the dried root and rhizome of Rheum palmatum L. ( dà huáng ), has therapeutic effect on experimental colitis by restoring intestinal microbial dysbiosis ( Wu et al, 2020 ). Moreover, qīng dài and huáng qín , as well as formulas such as Ginseng, Poria and Atractylodes Macrocephalae Powder ( Shēn Líng Bái Zhú Săn ), Coptis Toxin-Resolving Decoction ( Huáng Lián Jiĕ Dú Tāng ), and Stomach-Calming Powder ( Píng Wèi Săn ), can inhibit experimental colitis by restoring microbial diversity, increasing the abundance of beneficial bacteria, and inhibiting the growth of pathogenic bacteria ( Ma et al, 2019 ; Zhang Z. et al, 2020 ; Qi-yue et al, 2020 ; Yuan et al, 2020 ; Zhu et al, 2020 ). Short-chain fatty acids produced by intestinal microbiota are not only nutrients for microorganisms and immune regulation participants, but also the major energy source of colonic epithelial cells, and their production helps to enhance the function of the epithelial barrier ( Parada Venegas et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Efficacy and Safety of Probiotics Combined With Traditional Chinese Medicine for Ulcerative Colitis: A Systematic Review and Meta-Analysis

She

et al. 2022

Front. Pharmacol.

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Background: The combination of probiotics and traditional Chinese medicine (TCM) is a prospective therapy for ulcerative colitis (UC), and its efficacy and safety need to be urgently evaluated.Objective: This study aims to comprehensively assess the efficacy and safety of probiotics combined with TCM for the treatment of UC.Methods: The Pubmed, EMBASE, Cochrane library, China Academic Journals (CNKI), Wan-fang database, Chinese biomedical literature service system (CBM), and Chinese Science and Technology Journals (CQVIP) were searched. Subgroup analysis were designed in accordance with different control drugs, treatment courses, and types of probiotics. The Review Manager software (version 5.4.1) was utilized for statistical analysis.Results: 14 original studies containing 1,154 patients were analyzed and showed that probiotics with TCM was more effective than 5-aminosalicylic acid (5-ASA), probiotics or TCM used individually. Moreover, probiotics combined with TCM could inhibit the intestinal inflammation, reduce the recurrence rate and the incidence of adverse events. The subgroup analysis showed that a mixture of different probiotics was more effective than a single strain.Conclusion: It is suggested that probiotics combined with TCM could effectively control clinical symptoms, inhibit intestinal inflammatory response, and finally slow down the disease progress and reduce the disease recurrence with less adverse events. The mixture of different probiotics used in conjunction with individually tailored TCM is a potential clinical strategy for UC.

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Section: Resultsmentioning

confidence: 99%

Efficacy and Safety of Probiotics Combined With Traditional Chinese Medicine for Ulcerative Colitis: A Systematic Review and Meta-Analysis

She

et al. 2022

Front. Pharmacol.

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“…Not entirely consistent with these studies, we found that the relative abundance of Lachnospiraceae_NK4A136_group, unclassi ed_f_Lachnospiraceae and norank_f_Lachnospiraceae were signi cantly decreased, and that of Staphylococcus, Bacteroides, Prevotelaceae_UCG-001 and Eubacterium_ ssicatena_group were signi cantly increased. Recent hotspots suggest that correcting and restoring ora diversity and abundance plays a key role in resistance to intestinal in ammation, such as Baitouweng decoction [43], nitrate [42], Ping weisan [44], Saccharomyces boulardii [45]. In this study, ginsenoside Rg1 reversed the changes of relative abundance of Staphylococcus, Bacteroides, Eubacterium_ ssicatena_group, Lachnospiraceae_NK4A136_group and norank_f_Lachnospiraceae.…”

Section: Discussionmentioning

confidence: 56%

Ginsenoside Rg1 Ameliorated Colitis by Regulating the Homeostasis of M1/M2 Macrophage Polarization and Intestinal Flora

Long

Liu

Kang

et al. 2021

Preprint

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Background: Aberrant M1/M2 macrophage polarization and intestinal flora disruption are involved in the pathological processes associated with ulcerative colitis (UC). Ginsenoside Rg1 has good immunomodulatory and anti-inflammatory effects and is effective in treating UC of humans and animals. However, it is unclear how ginsenoside Rg1 regulate the homeostasis of M1/M2 macrophage polarization and intestinal flora.Methods: BALB/c mice were randomly divided into 4 groups: Control, DSS, DSS+Rg1, DSS+Y27632 groups. In this study, experiment colitis was induced in BALB/c mice using sodium dextran sulfate (DSS). Mice of DSS+Rg1, DSS+Y27632 groups were treated respectively with ginsenoside Rg1 and Rock inhibitor Y27632 for 14 consecutive days. On day 21, all mice were sacrificed. Histopathological analysis of the colon tissues was performed by Hematoxylin Eosin sinning. Cytokines (IL-6, IL-33, CCL-2, TNF-α, IL-4 and IL-10) were detected by Elisa. Flow cytometry was used to analyse macrophage activation and M1/M2 macrophage polarisation. Western blotting were applied to detect the levels of Macrophage polarization-associated protein (Arg-1, MIF-1, PIM-1, TLR2) and Nogo-B/RhoA signaling molecules (Rock1, RhoA and Nogo-B). The fecal microbial populations were analyzed using 16S gene sequencing. Results: After ginsenoside Rg1 and Y27632 treatment, the changes of body weight, colon length, colonic weight index and colonic mucosal injury of colitis mice were effectively improved, accompanied by less ulcer formation and inflammatory cell infiltration, lower levels of pro-inflammatory cytokines (IL-6, IL-33, CCL-2, TNF-α) and higher anti-inflammatory cytokines (IL-4 and IL-10). Importantly, the percentage of CD11b+F4/80+, CD11b+F4/80+Tim-1+, CD11b+F4/80+TLR4+, and CD11b+F4/80+iNOS+ cells and the expression levels of MIF-1 and PIM-1 proteins were down-regulated significantly after ginsenoside Rg1 and Y27632 treatment, and CD11b+F4/80+CD206+ and CD11b+F4/80+CD163+ cells and Arg-1 up-regulated significantly. Intestinal flora composition were effectively improved after administration of ginsenoside Rg1. The Nogo-B/RchoA signaling pathway were obviously inhibited after ginsenoside Rg1 and Y27632 treatment, and the levels of Rock1, RhoA and Nogo-B proteins were significantly reduced. Conclusions: Ginsenoside Rg1 has the protective effect on UC by inhibiting macrophage activation, restoring the balance of M1/M2 macrophage polarization, and improving intestinal flora composition, associated with inhibition of the Nogo-B/RhoA signaling pathway.

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“…The microbial dysbiosis in UC patients includes the decreased diversity and microbiota composition disorder ( 231 ). Dà Huáng Mŭ Dān Tāng (Rhubarb and peony bark Decoction) ( 223 ), P í ng Wèi S ă n (Stomach-Calming Powder) ( 225 ), Qing Cháng Huà Shī Tāng (Intestine-Clearing and Dampness-Removing Decoction) ( 226 ), Sān Huáng Shú Ài Tāng ( 228 ) and Bā wèi Xī lèi Sǎn ( 232 ) all could restore the diversity of the intestinal microbiota in DSS-treated mice. Meanwhile, it was found that the intestinal bacteria in DSS-treated mice also showed a recovery after administration: the abundance of Firmicutes increased, the amount of Bacteroides and Proteobacteria decreased, and the amount of lactobacillus increased.…”

Section: The Medication Of Ucmentioning

confidence: 99%

The Communication Between Intestinal Microbiota and Ulcerative Colitis: An Exploration of Pathogenesis, Animal Models, and Potential Therapeutic Strategies

et al. 2021

Front. Med.

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Ulcerative Colitis (UC) is a chronic inflammatory bowel disease. The prolonged course of UC and the lack of effective treatment management make it difficult to cure, affecting the health and life safety of patients. Although UC has received more attention, the etiology and pathogenesis of UC are still unclear. Therefore, it is urgent to establish an updated and comprehensive understanding of UC and explore effective treatment strategies. Notably, sufficient evidence shows that the intestinal microbiota plays an important role in the pathogenesis of UC, and the treating method aimed at improving the balance of the intestinal microbiota exhibits a therapeutic potential for UC. This article reviews the relationship between the genetic, immunological and microbial risk factors with UC. At the same time, the UC animal models related to intestinal microbiota dysbiosis induced by chemical drugs were evaluated. Finally, the potential value of the therapeutic strategies for restoring intestinal microbial homeostasis and treating UC were also investigated. Comprehensively, this study may help to carry out preclinical research, treatment theory and methods, and health management strategy of UC, and provide some theoretical basis for TCM in the treatment of UC.

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Ping weisan alleviates chronic colitis in mice by regulating intestinal microbiota composition

Cited by 25 publications

References 27 publications

Efficacy and Safety of Probiotics Combined With Traditional Chinese Medicine for Ulcerative Colitis: A Systematic Review and Meta-Analysis

Efficacy and Safety of Probiotics Combined With Traditional Chinese Medicine for Ulcerative Colitis: A Systematic Review and Meta-Analysis

Ginsenoside Rg1 Ameliorated Colitis by Regulating the Homeostasis of M1/M2 Macrophage Polarization and Intestinal Flora

The Communication Between Intestinal Microbiota and Ulcerative Colitis: An Exploration of Pathogenesis, Animal Models, and Potential Therapeutic Strategies

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