Pinopodes, leukemia inhibitory factor, integrin-β3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss

Xu, Bufang; Sun, Xiaoxi; Li, Lü; Wu, Ling; Zhang, Aijun; Feng, Yun

doi:10.1016/j.fertnstert.2012.04.032

Cited by 63 publications

(54 citation statements)

References 39 publications

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“…Our data indicate that women with unexplained RPL have significantly reduced integrin expression compared to controls. Our results differ from those published from Xu et al [15] and Tuckerman [17]. The probable cause for this discrepancy is the methodology.…”

Section: Discussioncontrasting

confidence: 99%

“…Reduced expression of αvβ3 has been related to unexplained infertility [11] and in women with endometriosis [14]. Xu et al , in a recent publication, did not find any difference in β3 integrin expression in women with RPL compared to normal fertile women [15]. However, these authors analysed the expression of β3 integrin in formalin fixed paraffin embedded (FFPE) samples.…”

Section: Introductionmentioning

confidence: 99%

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Endometrial beta3 Integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss

Germeyer

Savaris

Jauckus

et al. 2014

Reprod Biol Endocrinol

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BackgroundThe pathophysiology of recurrent pregnancy loss (RPL) is still unknown in 50% of the cases. Herein we measure the expression of beta3 integrin subunit, a well-known implantation marker, in women with or without RPL and correlate it with the histological dating of the endometrial tissue.MethodsLH-timed endometrial biopsies were obtained from cases (RPL; n = 21, age 33.9+/-4.7) and healthy controls (n = 29; age 29.8+/-4.1) during the mid-secretory phase (post ovulatory day: 8 to 10). Endometrial samples were timed histologically according to Noyes’ criteria and underwent immunohistochemical staining for beta3 integrin expression. For statistical analysis the semi-quantitative HSCORE was assessed. Type I (beta3 negative in an out-of-phase endometrium) and Type II defect (beta3 negative in an in-phase endometrium) were also analysed. Statistical analysis was done with Student t-test, Mann Whitney U test, ANCOVA and chi square for trend. Significance was set as P < 0.05.ResultsThe mean (SD) age in controls was lower compared to cases [(29.8 (4.1) vs. 33.9 (4.7) – P = 0.001; Student t-test)]. The median (range) expression of beta3 integrin in controls and cases was 1.94 (0 to 3.5) vs. 0.82 (0 to 3.6), respectively (P = 0.001; Mann Whitney U test). Significance was still significant after adjusting for age (P = 0.03;ANCOVA). The normal positive staining > =0.7 of beta3 integrin subunit and in-phase endometrium was seen in 24 out of 29 (82.8%) controls, but in only 6 out of 21 (28.6%) of cases with RPL; Type I and II defects were seen in 10.3 and 6.9% of controls, while present in 52.4 and 19.1% of cases, respectively (P = 0.0005; chi-square).ConclusionsWomen with unexplained RPL had significantly reduced integrin expression compared to controls. Our findings underline the need for further molecular analysis of endometrial tissue in affected women.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Endometrial beta3 Integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss

Germeyer

Savaris

Jauckus

et al. 2014

Reprod Biol Endocrinol

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“…Although both HOXA10 and ␣v␤3, markers of uterine receptivity in humans, have been reported to appear at the time of implantation in the human endometrium and decrease in women with endometriosis and infertility (15,40), the mechanism of HOXA10 and ␣v␤3 dysregulation remains unknown. Recent animal and human studies have reported HOXA10 hypermethylation as one of the possible mechanisms by which HOXA10 levels are decreased in endometriosis.…”

Section: Discussionmentioning

confidence: 99%

PCAF Impairs Endometrial Receptivity and Embryo Implantation by Down-Regulating β3-Integrin Expression via HOXA10 Acetylation

Zhu

Sun

et al. 2013

The Journal of Clinical Endocrinology & Metabolism

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“…Conversely, in humans, MUC1 levels increase during the midsecretory phase and peak 7 days after the luteal hormone (LH) surge to coincide with embryo implantation (Meseguer et al, 1998). Multiple studies have reported that patients suffering from recurrent implantation failure have significantly lower levels of MUC1 in the endometrium during the window of implantation, compared to healthy fertile women Xu et al, 2012;Bastu et al, 2015). Moreover, patients experiencing infertility due to hydrosalpinx, a condition in which the distal end of a fallopian tube is blocked with serous fluid, have exhibited lower MUC1 expression in the endometrium (Li et al, 2010;Song et al, 2012).…”

Section: Osteopontinmentioning

confidence: 99%

Molecular mechanisms of membrane interaction at implantation

Davidson

Coward

2016

Birth Defects Research Pt C

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Successful pregnancy is dependent upon the implantation of a competent embryo into a receptive endometrium. Despite major advancement in our understanding of reproductive medicine over the last few decades, implantation failure still occurs in both normal pregnancies and those created artificially by assisted reproductive technology (ART). Consequently, there is significant interest in elucidating the etiology of implantation failure. The complex multistep process of implantation begins when the developing embryo first makes contact with the plasma membrane of epithelial cells within the uterine environment. However, although this biological interaction marks the beginning of a fundamental developmental process, our knowledge of the intricate physiological and molecular processes involved remains sparse. In this synopsis, we aim to provide an overview of our current understanding of the morphological changes which occur to the plasma membrane of the uterine endothelium, and the molecular mechanisms that control communication between the early embryo and the endometrium during implantation. A multitude of molecular factors have been implicated in this complex process, including endometrial integrins, extracellular matrix molecules, adhesion molecules, growth factors, and ion channels. We also explore the development of in vitro models for embryo implantation to help researchers investigate mechanisms which may underlie implantation failure. Understanding the precise molecular pathways associated with implantation failure could help us to generate new prognostic/diagnostic biomarkers, and may identify novel therapeutic targets.

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Pinopodes, leukemia inhibitory factor, integrin-β3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss

Cited by 63 publications

References 39 publications

Endometrial beta3 Integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss

Endometrial beta3 Integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss

PCAF Impairs Endometrial Receptivity and Embryo Implantation by Down-Regulating β3-Integrin Expression via HOXA10 Acetylation

Molecular mechanisms of membrane interaction at implantation

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