Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism

Huang, Xiangjie; Xue, Zexin; Zhang, Delong; Lee, Hyeon Jeong

doi:10.1021/acs.analchem.4c01398

Cited by 2 publications

References 118 publications

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Alteration of Lipid Metabolism in Hypoxic Cancer Cells

Gonzalez,

Osuji,

Fiur

et al. 2024

Chemical & Biomedical Imaging

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Due to uncontrolled cell proliferation and disrupted vascularization, many cancer cells in solid tumors have limited oxygen supply. The hypoxic microenvironments of tumors lead to metabolic reprogramming of cancer cells, contributing to therapy resistance and metastasis. To identify better targets for the effective removal of hypoxia-adaptive cancer cells, it is crucial to understand how cancer cells alter their metabolism in hypoxic conditions. Here, we studied lipid metabolic changes in cancer cells under hypoxia using coherent Raman scattering (CRS) microscopy. We discovered the accumulation of lipid droplets (LDs) in the endoplasmic reticulum (ER) in hypoxia. Time-lapse CRS microscopy revealed the release of old LDs and the reaccumulated LDs in the ER during hypoxia exposure. Additionally, we explored the impact of carbon sources on LD formation and found that MIA PaCa2 cells preferred fatty acid uptake for LD formation, while glucose was essential to alleviate lipotoxicity. Hyperspectralstimulated Raman scattering (SRS) microscopy revealed a reduction in cholesteryl ester content and a decrease in lipid saturation levels of LDs in hypoxic MIA PaCa2 cancer cells. This alteration in LD content is linked to reduced efficacy of treatments targeting cholesteryl ester formation. This study unveils important lipid metabolic changes in hypoxic cancer cells, providing insights that could lead to better treatment strategies for hypoxia-resistant cancer cells.

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Alteration of Lipid Metabolism in Hypoxic Cancer Cells

Gonzalez,

Osuji,

Fiur

et al. 2024

Chemical & Biomedical Imaging

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Vitamin A imaging in mammalian cells by hyperspectral stimulated Raman scattering microscopy

Huang,

Hou,

et al. 2024

Optics in Health Care and Biomedical Optics XIV

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Vitamin A, a fat-soluble vitamin essential for various biological processes, exists in multiple metabolic forms that are primarily converted in the liver. This complex and organ-specific metabolism depends on subcellular localization for proper conversion and function. Even within a single cell, the distribution of vitamin A is highly heterogeneous.Leveraging sub-micron spatial resolution and chemical selectivity, hyperspectral stimulated Raman scattering microscopy (hSRS) has been widely used to visualize metabolites in complex biological samples. Here, we employ (hSRS) imaging in fingerprint regions to visualize vitamin A distribution in mammalian cells.

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Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism

Abstract: B) SRS spectra of saturated fatty acid standards with different chain lengths, linear fitting curve for AUC ratio of CH 2 /CH 3 bonds as a function of chain length, and chain length prediction in E. coli.

Cited by 2 publications

References 118 publications

Alteration of Lipid Metabolism in Hypoxic Cancer Cells

Alteration of Lipid Metabolism in Hypoxic Cancer Cells

Vitamin A imaging in mammalian cells by hyperspectral stimulated Raman scattering microscopy

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