Pioglitazone increases the numbers and improves the functional capacity of endothelial progenitor cells in patients with diabetes mellitus

Wang, Chao-Hung; Ting, Ming-Kuo; Verma, Subodh; Kuo, Li‐Tang; Yang, Ning-I; Hsieh, I‐Chang; Wang, Shin-Yi; Hung, Agnes; Cherng, Wen‐Jin

doi:10.1016/j.ahj.2006.07.029

Cited by 108 publications

(95 citation statements)

References 29 publications

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“…10 -12 Recently, it has been reported that treatment of diabetic patients with the PPAR␥ ligands, possibly by modulating subclinical inflammatory activity or attenuating the detrimental effects of C reactive protein (CRP), increases the number and improves the functional capacity of endothelial progenitor cells (EPCs), thus providing evidence for PPAR␥-mediated vascular protection. 13 …”

mentioning

confidence: 99%

Formation of STAT5/PPARγ Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes

Dentelli

Trombetta

Togliatto

et al. 2009

ATVB

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Objective-Circulating angiogenic cells (CACs) expansion is a multistage process requiring sequential activation of transcriptional factors, including STAT5. STAT5, in concert with peroxisome proliferator-activated receptors (PPARs), seems to induce discrete biological responses in different tissues. In the present study we investigated the role of STAT5 and PPAR␥ in regulating CAC expansion in normal and diabetic settings. Methods and Results-Normal

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mentioning

confidence: 99%

Formation of STAT5/PPARγ Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes

Dentelli

Trombetta

Togliatto

et al. 2009

ATVB

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“…As an aside, we had the possibility to evaluate about 30-50 times more events in this study compared with most of the previous reports. 15,16,[18][19][20] …”

Section: Discussionmentioning

confidence: 99%

“…So far, the investigation of EPC number in relation to hsCRP or hypertension led to heterogeneous results. [14][15][16][18][19][20] However, for determination of extremely rare eventsFas is the case in EPC analysisFa very high number of PBMCs must be analyzed to obtain reliable results. As an aside, we had the possibility to evaluate about 30-50 times more events in this study compared with most of the previous reports.…”

Section: Discussionmentioning

confidence: 99%

Reduction of both number and proliferative activity of human endothelial progenitor cells in obesity

et al. 2010

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Objective: Circulating endothelial progenitor cells (EPCs), responsible for neoangiogenesis and vascular repair, negatively correlate with vascular dysfunction and atherosclerotic risk factors. Because obesity may have a crucial role in the development of endothelial dysfunction, this study evaluated the number and proliferative activity of circulating human EPCs in obese (body mass index (BMI) ¼ 48±9, n ¼ 45) compared with lean (23±2, n ¼ 45) volunteers. Methods: EPCs were quantified after isolation of peripheral blood mononuclear cells (PBMCs) using fluorescence-activated cell sorting analyses. In addition, plated PBMCs developed colony-forming units (CFUs) from which 'outgrowth' endothelial cells (OECs) sprouted and differentiated into mature endothelial cells. Growth rates were monitored by periodical microscopic evaluation. Cell-cycle protein expression was determined by western blot analyses. Results: BMI negatively correlated (Po0.01) with the number of.500) and CD133 þ /KDR þ (r ¼ À0.282) EPCs. Insulin, leptin, HbA 1c , high-sensitivity C-reactive protein and hypertension, as well as diminished high-density lipoprotein and apolipoprotein A1, were not only associated with obesity but also with significantly reduced EPC levels. Applying selective culture conditions, EPC-CFUs differentiated into OECs that proliferated more slowly when derived from obese compared with lean subjects (obese: 19.9 ± 2.2% vs lean: 30.9 ± 3.2% grown area per week, Po0.01). The reduced proliferation was reflected by decreased (Po0.05, n ¼ 24 for each group) expression of cell-cyclepromoting cyclins and E2F-1, by hypophosphorylation of retinoblastoma protein and by increased (Po0.05, n ¼ 24 for each group) expression of the cell-cycle inhibitor p21 WAFÀ1/Cip1 . Conclusions: Reduced numbers of EPCs along with their premature senescence, as shown in this study, could function as early contributors to the development and progression of vascular dysfunction in obesity.

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“…28,35,59,72 Glucagon-like peptide-1 agonist and DPP-4 inhibitors are a newer class of antidiabetic agents that act by increasing the Figure 2. Potential therapeutic avenues to restore endothelial progenitor cell (EPC) number and functions for the prevention of macrovascular and microvascular complications in diabetes mellitus (DM).…”

Section: Antidiabetic Agentsmentioning

confidence: 99%

Specific Role of Impaired Glucose Metabolism and Diabetes Mellitus in Endothelial Progenitor Cell Characteristics and Function

Yiu

Tse

2014

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Abstract-The disease burden of diabetes mellitus (DM) and its associated cardiovascular complications represent a growing and major global health problem. Recent studies suggest that circulating exogenous endothelial progenitor cells (EPCs) play an important role in endothelial repair and neovascularization at sites of injury or ischemia. 1 This has been attributed to the occurrence of endothelial dysfunction that leads to the initiation and progression of atherosclerotic vascular disease 2 and impaired neovascularization after ischemia induced by hyperglycemia. 3,4 In patients with impaired glucose metabolism and DM, the vascular endothelium is challenged by inflammation, reactive oxygen species, and deletion of endothelial nitric oxide synthase (eNOS) with resultant endothelial dysfunction.2,5-7 The depletion and dysfunction of the circulating endothelial progenitor cells (EPCs) are thought to underlie the endothelial dysfunction in DM. In this review, the possible mechanisms, functional consequences, and the potential therapeutic approach for impaired EPCs in DM are discussed. A systematic literature search for full-text papers in the English language was performed using MEDLINE, Embase, and the Cochrane library through to February 2014. In the search phrases used, the following terms were combined with the phrase AND Diabetes: endothelial progenitor cells, cardiovascular disease, myocardial infarction, and stroke.

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Pioglitazone increases the numbers and improves the functional capacity of endothelial progenitor cells in patients with diabetes mellitus

Cited by 108 publications

References 29 publications

Formation of STAT5/PPARγ Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes

Formation of STAT5/PPARγ Transcriptional Complex Modulates Angiogenic Cell Bioavailability in Diabetes

Reduction of both number and proliferative activity of human endothelial progenitor cells in obesity

Specific Role of Impaired Glucose Metabolism and Diabetes Mellitus in Endothelial Progenitor Cell Characteristics and Function

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