Piperacillin Estimation by Ion-Associative Complex Formation

Abstract

Objective: The objective of the study was to develop a simple, validated, and affordable visible spectrophotometric method for determination of piperacillin (PIP) present in bulk and powder for injection formulation.
 Methods: In the present method, cobalt thiocyanate (CTC) was used as a chromogenic reagent where it forms 2:1 ion pair complex at pH 2 with PIP which is having secondary and tertiary amine groups.
 Results: The formed bluish-green colored ion pair between PIP and CTC is quantitatively extractable into nitrobenzene with an absorption maximum of 665 nm. Regression analysis (r=0.9996) shows that the plotted calibration curve exhibits good linearity in the studied range of concentration (3–18 μg/mL). Low values of relative standard deviation (<2%) were observed indicating that the proposed method is reproducible, accurate, and precise.
 Conclusions: As per the existing guidelines of ICH (international council for harmonization of technical requirements for pharmaceuticals for human use), various parameters of the proposed method were tested for validation and can be used method of choice for routine analysis in industrial quality control laboratories, especially in developing countries.

Ninhydrin Based Visible Spectrophotometric Determination of Gemigliptin

“…Then the amount of API was determined by adopting the above method (Table 6). Visible spectrophotometric method is the generally used for routine analysis in industrial QC laboratories, especially in developing countries [43][44][45][46][47][48][49] . Hence, the developed visible spectrophotometric method can be adopted for the determination of gemigliptin in pure and tablet formulations using Ninhydrin as a chromogenic agent.…”

Visible Spectrophotometric Determination of Gemigliptin using Charge Transfer Complex

“…Out of the various reactions involved in spectrophotometric assay of pharmaceutical drugs, charge transfer reaction is proved to be highly sensitive as well as acceptable method, in addition to reactions involving to ion pair [9][10][11][12][13][14][15] and oxidation [16][17][18][19][20] . The complex formed between electron donor and acceptor compound yields a new absorption band with a precise l max pertaining to that complex.…”

Determination of Riociguat by Oxidative Coupling Using Visible Spectrophotometry

“…But, not reported any visible spectrophotometric method. Well known spectrophotometric methods involve ion pair formation [10][11][12][13][14][15] and oxidation [16][17][18][19][20] . Hence, an attempt was made in the current study to verify the applicability of a chromogenic coupling agent like MBTH (3-methylbenzothiazolinone hydrazone) as an analytical probe to develop as well as validate a simple visible spectrophotometric method to determine Riociguat.…”