Piperine as an adjuvant increases the efficacy of curcumin in mitigating benzo(a)pyrene toxicity

Abstract

In the present study, the antioxidative and anticlastogenic effects of curcumin and piperine separately and in combination have been investigated against benzo(a)pyrene (BaP)-mediated toxicity in mice. Male Swiss albino mice were pretreated with curcumin (100 mg kg(-1) body weight) and piperine (20 mg kg(-1) body weight) separately as well as in combination orally in corn oil for 7 days; and subsequently, after 2 h of pretreatment, BaP was administered orally in corn oil (125 mg kg(-1) body weight). A single dose of BaP in normal mice increased the levels of lipid peroxidation (LPO), protein carbonyl content (PCC), and frequency of bone marrow micronucleated polychromatic erythrocytes (MNPCEs) but decreased significantly the levels of endogenous antioxidants such as superoxide dismutases (SODs), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) and reduced glutathione (GSH) in the liver. Pretreatments with curcumin and curcumin plus piperine before administration of single dose of BaP significantly decreased the levels of LPO, PCC, and incidence of MNPCEs but elevated the level of GSH and enzyme activities of GPx, GR, SOD, CAT, and glutathione-S-transferase (GST) when compared to the BaP-treated group. The effect of curcumin plus piperine is more pronounced as compared to curcumin in attenuating BaP-induced oxidative insult and clastogenicity.

Effect of Curcumin Against Oxidation of Biomolecules by Hydroxyl Radicals

“…This was in agreement with Biswas SK et al, [45], who stated that curcumin can induce glutathione synthesis and increase the level of GSH and glutamylcysteine ligase catalytic subunit mRNA expression in oxidant and TNF α treated cells. The results in this study indicates that, curcumin may have profound effects on cellular redox control and similar to the studies of Eybl V et al, [36] and Sehgal A et al, [46].…”

Antigenotoxic capacity of beta-caryophyllene in mouse, and evaluation of its antioxidant and GST induction activities

“…The produced metabolites may damage DNA by forming adducts, but also through the induction of ROS that, besides DNA, may also damage proteins and lipids. These molecular alterations can be the subjacent origin of the presently observed increase in the number of SCE and CA, result which is in line with the genotoxic potential of BaP previously demonstrated in in vitro and in vivo short-term tests (Alvarez-González et al, 2011;Bhagavathy and Sumathi, 2012;Sehgal et al, 2012). In the case of BC, our results showed no capacity of the chemical to induce SCE and CA in mouse, a finding which is concordant with previous studies using bacterial tests, and the analysis of micronuclei in vitro and in vivo (Di Sotto et al, 2008Molina-Jasso et al, 2009).…”

“…Respect to the first mentioned mechanism, it is pertinent to stress that a single administration of the mutagen resulted in a significant increase of lipoperoxidation and protein oxidation, suggesting an initial induction of oxidative stress in hepatic tissue that could be disseminated to all cells of the organism. In fact, BaP has been previously reported to increase lipoperoxidative products either by producing ROS or by decreasing the activities of antioxidant enzymes, as well as to increase the level of oxidized proteins in the liver of mice (Emre et al, 2007;Sehgal et al, 2012). These effects may lead to serious cellular damage including modification of cell structure, enzymatic activity, signaling pathways, and DNA damage Kryston et al, 2011).…”

Synergistic effect of curcumin and piperine in suppression of DENA-induced hepatocellular carcinoma in rats

“…Several studies showed its anticancer potential against different types of hepatic, gastrointestinal, skin, lung, hematologic malignancies and many other types of cancers (Sehgal et al, 2012;Abouzied et al, 2015). Curcumin has been reported to exhibit its anticancer activity primarily through inhibition of carcinogen activation, stimulation of carcinogen detoxification, suppression of pro-inflammatory signaling, induction of cancer cell apoptosis, cell cycle arrest, inhibition of angiogenesis and metastasis, modulation of oncogenes and tumor suppressor genes (Rajasekaran, 2011).…”