Piriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus carica

Amani, Shahla; Mohebodini, Mehdi; Khademvatan, Shahram; Jafari, Morad; Kumar, Vinod

doi:10.1016/j.jbiotec.2020.12.015

Cited by 13 publications

(8 citation statements)

References 64 publications

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“…In our previous study, although due to the lack of expression of F3’H and BHLH genes in the control sample, we failed to analyze the expression of these genes after elicitation, but we found that fungal elicitation was able to up-regulate PAL , CHS , and UFGT and MBYB3 genes in hairy root cultures of F. carica cv. Sabz [ 8 ]. Based on this study, in F. carica cv.…”

Section: Discussionmentioning

confidence: 99%

“…The gallic acid, chlorogenic acid, caffeic acid, ferulic acid, coumaric acid, syringic acid, and quinol, are the main phenolic acids and catechin, kaempferol, quercitin, rutin, and myricetin are the main flavonoids of F. carica [ 1 , 4 ]. These phytochemicals (phenolic and flavonoid compounds) are synthesized through the phenylpropanoid and flavonoid pathway [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Modifications in gene expression and phenolic compounds content by methyl jasmonate and fungal elicitors in Ficus carica. Cv. Siah hairy root cultures

Amani,

Mohebodini,

Khademvatan

et al. 2024

BMC Plant Biol

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Background One of the most effective strategies to increase phytochemicals production in plant cultures is elicitation. In the present study, we studied the effect of abiotic and biotic elicitors on the growth, key biosynthetic genes expression, antioxidant capacity, and phenolic compounds content in Rhizobium (Agrobacterium) rhizogenes-induced hairy roots cultures of Ficus carica cv. Siah. Methods The elicitors included methyl jasmonate (MeJA) as abiotic elicitor, culture filtrate and cell extract of fungus Piriformospora indica as biotic elicitors were prepared to use. The cultures of F. carica hairy roots were exposed to elicitores at different time points. After elicitation treatments, hairy roots were collected, and evaluated for growth index, total phenolic (TPC) and flavonoids (TFC) content, antioxidant activity (2,2-diphenyl-1-picrylhydrazyl, DPPH and ferric ion reducing antioxidant power, FRAP assays), expression level of key phenolic/flavonoid biosynthesis genes, and high-performance liquid chromatography (HPLC) analysis of some main phenolic compounds in comparison to control. Results Elicitation positively or negatively affected the growth, content of phenolic/flavonoid compounds and DPPH and FRAP antioxidant activities of hairy roots cultures in depending of elicitor concentration and exposure time. The maximum expression level of chalcone synthase (CHS: 55.1), flavonoid 3′-hydroxylase (F3’H: 34.33) genes and transcription factors MYB3 (32.22), Basic helix-loop-helix (bHLH: 45.73) was induced by MeJA elicitation, whereas the maximum expression level of phenylalanine ammonia-lyase (PAL: 26.72) and UDP-glucose flavonoid 3-O-glucosyltransferase (UFGT: 27.57) genes was obtained after P. indica culture filtrate elicitation. The P. indica elicitation also caused greatest increase in the content of gallic acid (5848 µg/g), caffeic acid (508.2 µg/g), rutin (43.5 µg/g), quercetin (341 µg/g), and apigenin (1167 µg/g) phenolic compounds. Conclusions This study support that elicitation of F. carica cv. Siah hairy roots can be considered as an effective biotechnological method for improved phenolic/flavonoid compounds production, and of course this approach requires further research.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modifications in gene expression and phenolic compounds content by methyl jasmonate and fungal elicitors in Ficus carica. Cv. Siah hairy root cultures

Amani,

Mohebodini,

Khademvatan

et al. 2024

BMC Plant Biol

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“…The most effective phenolic biosynthesis stimulant was the P. indica culture filtrate, at 2% ( v / v ) concentration, that was a potent inducer of PAL expression. The lowest concentration of both the fungal cell extract and the fungal culture filtrate strongly promoted the growth of the HRs [ 52 ].…”

Section: Phenolic and Polyphenolic Antioxidants In Hrsmentioning

confidence: 99%

Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates

Malarz

Yudina

Stojakowska

2023

IJMS

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Plant-derived antioxidants are intrinsic components of human diet and factors implicated in tolerance mechanisms against environmental stresses in both plants and humans. They are being used as food preservatives and additives or ingredients of cosmetics. For nearly forty years, Rhizobium rhizogenes-transformed roots (hairy roots) have been studied in respect to their usability as producers of plant specialized metabolites of different, primarily medical applications. Moreover, the hairy root cultures have proven their value as a tool in crop plant improvement and in plant secondary metabolism investigations. Though cultivated plants remain a major source of plant polyphenolics of economic importance, the decline in biodiversity caused by climate changes and overexploitation of natural resources may increase the interest in hairy roots as a productive and renewable source of biologically active compounds. The present review examines hairy roots as efficient producers of simple phenolics, phenylethanoids, and hydroxycinnamates of plant origin and summarizes efforts to maximize the product yield. Attempts to use Rhizobium rhizogenes-mediated genetic transformation for inducing enhanced production of the plant phenolics/polyphenolics in crop plants are also mentioned.

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“…It can even colonize Arabidopsis thaliana , a cruciferous model plant that AM fungi cannot colonize (Abdelaziz et al, 2017 ; Manzar et al, 2021b ). Many scholars have studied the colonization of P. indica in various plants, focusing on its effects on promoting host plant growth, improving disease resistance, and enhancing stress tolerance, including resistance to drought, cold, heat, salt, and flooding stresses (Ghabooli et al, 2013 ; Amani et al, 2021 ). However, research on the basic biological characteristics of P. indica , such as its growth dynamics on different culture media, has been limited and has only been focused on screening suitable media at a macro level.…”

Section: Introductionmentioning

confidence: 99%

Analysis of growth dynamics in five different media and metabolic phenotypic characteristics of Piriformospora indica

Hu,

Li,

Wang

et al. 2024

Front. Microbiol.

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Piriformospora indica is an important endophytic fungus with broad potential for alleviating biotic and abiotic stress on host plants. This study monitored the growth dynamics of P. indica on five commonly used artificial media for microorganisms and analyzed its metabolic characteristics using Biolog Phenotype Microarray (PM) technology. The results showed that P. indica grew fastest on Potato Dextrose Agar (PDA), followed by Kidney Bean Agar (KBA), Alkyl Ester Agar (AEA), Oatmeal Agar (OA), and Luria-Bertani Agar (LB), and the most suitable medium for spore production was OA. Using Biolog PM1-10, 950 metabolic phenotypes of P. indica were obtained. P. indica could metabolize 87.89% of the tested carbon sources, 87.63% of the tested nitrogen sources, 96.61% of the tested phosphorus sources, and 100% of the tested sulfur sources. P. indica displayed 92 kinds of tested biosynthetic pathways, and it could grow under 92 kinds of tested osmotic pressures and 88 kinds of tested pH conditions. PM plates 1-2 revealed 43 efficient carbon sources, including M-Hydroxyphenyl acid, N-Acetyl-D-Glucosamine, Tyramine, Maltotrios, α-D-Glucosine, I-Erythritol, L-Valine, D-Melezitose, D-Tagatose, and Turanose. PM plates 3,6-8 indicated 170 efficient nitrogen sources, including Adenosine, Inosine Allantoin, D, L-Lactamide, Arg-Met, lle-Trp, Ala-Arg, Thr-Arg, Trp-Tyr, Val-Asn, Gly-Gly-D-Leu, Gly-Gly-Phe, and Leu-Leu-Leu. This study demonstrates that P. indica can metabolize a variety of substrates, such as carbon and nitrogen sources, and has a wide range of environmental adaptability. The growth dynamics on artificial culture media and metabolic phenotypes of P. indica can be used to investigate its biological characteristics, screen for more suitable growth and sporulation conditions, and elucidate the physiological mechanisms that enhance the stress resistance of host plants. This study provides a theoretical basis for its better application in agriculture.

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Piriformospora indica based elicitation for overproduction of phenolic compounds by hairy root cultures of Ficus carica

Cited by 13 publications

References 64 publications

Modifications in gene expression and phenolic compounds content by methyl jasmonate and fungal elicitors in Ficus carica. Cv. Siah hairy root cultures

Modifications in gene expression and phenolic compounds content by methyl jasmonate and fungal elicitors in Ficus carica. Cv. Siah hairy root cultures

Hairy Root Cultures as a Source of Phenolic Antioxidants: Simple Phenolics, Phenolic Acids, Phenylethanoids, and Hydroxycinnamates

Analysis of growth dynamics in five different media and metabolic phenotypic characteristics of Piriformospora indica

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