Pitfalls and promises in FTIR spectromicroscopy analyses to monitor iron-mediated DNA damage in sperm

Pascolo, Lorella; Bedolla, Diana E.; Vaccari, Lisa; Venturin, Irene; Cammisuli, Francesca; Gianoncelli, Alessandra; Mitri, Elisa; Giolo, Elena; Luppi, Stefania; Martinelli, Monica; Zweyer, Marina; Ricci, Giuseppe

doi:10.1016/j.reprotox.2016.02.011

Cited by 21 publications

(14 citation statements)

References 45 publications

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“…On the other hand, the explanation for the absence of spectral features indicating DNA damage after UV-irradiation is, as yet, not easy. Pascolo et al [ 36 ] using FTIR spectromicroscopy on sperm samples exposed to oxidative stress mediated by Fenton’s reaction, reported that the DNA damage interpretation is partially compromised. Unexpected cell surface iron-oxygen precipitates were generated during Fenton’s reaction and generated a broad band which partially occupies the DNA phosphate stretching bands.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, it is known that presence of ions and water are essential in determining the conformation of a nucleic acid [ 37 ]. For instance, by Fourier-transform infrared spectroscopy (FTIR) it was proven that dehydration induces the transition of DNA from the natural B- to the A-helical conformation [ 36 ]. However, a conformational change in the DNA molecule is most likely not the reason explaining our observations.…”

Section: Discussionmentioning

confidence: 99%

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Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration

Costa¹,

Amaral²,

Redmαnn³

et al. 2018

PLoS ONE

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Raman Microspectroscopy represents an innovative tool for the assessment of sperm biochemical features otherwise undetectable by routine semen analysis. Previously, it was shown that induced DNA damage can be detected in smeared sperm by this technique. This novel readout may be of value for clinical settings especially if it can be transferred to living cells. Yet, starting with living sperms this study was carried-out using a variety of conditions to disclose the Raman features of sperm nuclei under different hydration conditions and UV exposure. Human sperm were immobilized and Raman spectra were obtained from individual sperm as repeated measurements. To create conditions with controlled DNA damage, sperm samples were exposed to ultraviolet light. Several media were used to evaluate their effect on Raman spectra in aqueous conditions. To substantiate differences between the experimental conditions, the spectra were analyzed by Principal Component Analysis. We observed that spectra of sperm nuclei obtained in different solutions showed a qualitatively unchanged spectral pattern showing the principal signals related to DNA. Evaluating the effect of ultraviolet light generated the finding that spectra representing DNA damage were only observed in dry conditions but not in aqueous medium. Thus, Raman microspectroscopy was successfully applied for sperm analysis in different conditions, among them in live spermatozoa in aqueous solution during the initial measurement, revealing the principle use of this technique. However, implementation of Raman spectroscopy as a technique for clinical sperm analysis and selection may be especially relevant when DNA evaluation can be established using live sperm.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration

Costa¹,

Amaral²,

Redmαnn³

et al. 2018

PLoS ONE

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“…The infrared spectrum was recorded with 4 cm −1 resolution and 32 scans at wavelength range of 4000–400 cm −1 . The carbon dioxide and water vapour in the spectrum of the original sample were calibrated using the atmospheric compensation program of the OPUS 7.0 software (Bruker Optics GmbH, Ettlingen, Germany) (Pascolo et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

Teng

et al. 2018

Int J of Food Sci Tech

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This study aimed to synthesise a complex of agar oligosaccharides (AOS) with ferric iron and to evaluate its physicochemical and structural characteristics. Based on single factor, Plackett-Burman and response surface optimal experiments of three factors at three levels, the optimised chelate conditions of the AOSiron (III) complex were mass ratio of AOS to iron ions of 4.5:1 g:g, reaction temperature of 74°C and reaction time of 60 min. Under these conditions, the iron content and total sugar content were 14.03 AE 0.42% and 66.07 AE 0.96%, respectively. The physicochemical analysis showed that AOS-iron (III) was soluble and steady at physiological pH, and it was easily reduced to iron (II). The structural characteristics of AOS and AOS-iron (III) were analysed by Raman spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction and Nuclear magnetic resonance, and results showed that the iron (III) ions were chelated to AOS by -OH groups and -COOH groups and widely dispersed on the AOS backbone. Therefore, AOS-iron (III) could be a candidate for iron supplementation to treat iron deficiency anaemia.

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“…Hexagonal packing of DNA and RNA was observed for DNA and RNA complexes with Cetyltrimethyl Ammonium Bromide (CTBA) and for Dimethyldioctadecyl Ammonium Bromide (DDAB) complexes is observed lamellar structure. Variations in the internal spacing and degree of long-range ordering are dependent on both surfactant type and concentrations of added salt [19][20][21][22][23][24][25][26][27][28][29]. When we increased the amount of salt into our complexes, we observed that the d spacing (d=2Π/r) are increased.…”

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confidence: 99%

“…Gemini surfactant is a name assigned to a family of synthetic amphiphiles surfactants such as: Alkanediyl-α-ω-bis (dimethylammonium bromide) surfactants referred to as m-s-m where s is the number of Carbon atoms in the polymethylene chains connecting the two CnH2n+1N+(CH3)2Br-moieties [30][31][32][33][34][35][36][37][38]. We present measurements of isothermal DNA/RNA +12-s-12 (with s=2, 4, 6, 8, 10,12,14,16,18,20) complexes at room temperature. This calorimeter provides simultaneous measurement of (a) Water activity (sorption isotherms) and (b) The partial molar enthalpy of water as a function of water uptake.…”

mentioning

confidence: 99%

A Thermodynamic Study on Hydration and Dehydration of DNA and RNA −Amphiphile Complexes

Heidari¹

2016

J Bioengineer & Biomedical Sci

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Pitfalls and promises in FTIR spectromicroscopy analyses to monitor iron-mediated DNA damage in sperm

Cited by 21 publications

References 45 publications

Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration

Spectral features of nuclear DNA in human sperm assessed by Raman Microspectroscopy: Effects of UV-irradiation and hydration

Preparation and characterisation of a novel agar oligosaccharide–iron (III) complex

A Thermodynamic Study on Hydration and Dehydration of DNA and RNA −Amphiphile Complexes

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