Pixelar index: a new quantitative approach for the assessment of reproductive condition in the purple sea urchin, Paracentrotus lividus, by image analysis

Mantilla-Aldana, Leonardo; Campoy-López, Pedro; Pereira-Pinto, Estefanía; Beiras, Ricardo

doi:10.1007/s10499-019-00499-8

Cited by 2 publications

(4 citation statements)

References 40 publications

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“…The histological analysis of the gonads would therefore reduce the high inter individual variability observed in these types of studies. The use of continuous indexes for the definition of the maturation stage, such as the GAF, using stereology or other techniques (Bayne et al, 1978;Mantilla-Aldana et al, 2019;Popović et al, 2013) is also recommended to obtain more precise and objective determinations of the degree of maturity of the gonads.…”

Section: Discussionmentioning

confidence: 99%

No evidence that vitellogenin protein expression is induced in marine mussels after exposure to an estrogenic chemical

Fernández-González

Diz

Grueiro³

et al. 2020

Science of The Total Environment

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Section: Discussionmentioning

confidence: 99%

No evidence that vitellogenin protein expression is induced in marine mussels after exposure to an estrogenic chemical

Fernández-González

Diz

Grueiro³

et al. 2020

Science of The Total Environment

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“…In 2016, females exposed to 10 µg/L TCPP showed significant higher values than control regardless of exposure time (P = 0.039 on day 7 and P = 0.006 on day 28). Significant differences were also observed in males, with an increase in PI values at a The Pixelar Index is a quantitative and objective tool used for the assessment of the stage of development in male and female gonads [49]. In 2016, females exposed to 10 µg/L TCPP showed significant higher values than control regardless of exposure time (P = 0.039 on day 7 and P = 0.006 on day 28).…”

Section: Gonad Growth and Pixelar Indexmentioning

confidence: 99%

“…The stained gonad sections were photographed at high resolution (total magnification100x) using TIFF format, and analyzed using the Pixelar Index (PI) developed by Mantilla-Aldana, et al [49], following the expression:…”

Section: Chemicals and Stock Solutionsmentioning

confidence: 99%

“…The patterns observed in the 2016 experiments were not confirmed in the 2017 experiments (Figure 4) conducted with sea urchins in a more advanced gametogenic stage (see Figure 2), nor were clear trends in GI observed that year (Figure 4a,b). The Pixelar Index is a quantitative and objective tool used for the assessment of the stage of development in male and female gonads [49]. In 2016, females exposed to 10 µg/L TCPP showed significant higher values than control regardless of exposure time (P = 0.039 on day 7 and P = 0.006 on day 28).…”

Section: Gonad Growth and Pixelar Indexmentioning

confidence: 99%

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Effects of Ethinylestradiol (EE2) and an Organophosphorus Flame Retardant (TCPP) on Gonadal Maturation in the Sea Urchin, Paracentrotus lividus

Campoy-López

Pereira-Pinto

Mantilla-Aldana

et al. 2020

JMSE

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The sea urchin (Paracentrotus lividus) was used to test the effects of one of the most abundant flame retardant additives for plastics, tris (1-chloro-2-propyl) phosphate (TCPP), and the synthetic hormone ethinylestradiol (EE2) on gametogenesis and gonad development of adults. With this aim, 403 individuals of both sexes were exposed to TCPP concentrations ranging from 0.2 to 10 µg/L, EE2 (0.01 µg/L), seawater and solvent controls for 7 and 28 days. EE2 and TCPP exposure did not cause histological damage in the gonads. Some evidence of estrogenic effects of TCPP within the µg/L range and EE2 within the ng/L range is reported. Females exposed to 1 µg/L TCPP for 7 days showed a significant increase in gonad development assessed as gametogenic stage, females exposed to 10 µg/L TCPP showed increased gonad development both in terms of weight (Gonad Index, GI, at both 7 and 28 days) and maturation (Pixelar Index, PI), and females exposed to 10 ng/L EE2 showed increased PI after 28 days exposure. Male sea urchins exposed to both TCPP and EE2 for 7 days showed increased frequencies of low development gonad stage. However, the patterns of response are affected by the high inter-individual variability, the differing initial stage of the gonad, as well as the dosage administered.

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Pixelar index: a new quantitative approach for the assessment of reproductive condition in the purple sea urchin, Paracentrotus lividus, by image analysis

Cited by 2 publications

References 40 publications

No evidence that vitellogenin protein expression is induced in marine mussels after exposure to an estrogenic chemical

No evidence that vitellogenin protein expression is induced in marine mussels after exposure to an estrogenic chemical

Effects of Ethinylestradiol (EE2) and an Organophosphorus Flame Retardant (TCPP) on Gonadal Maturation in the Sea Urchin, Paracentrotus lividus

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