PKA isoforms coordinate mRNA fate during nutrient starvation

Tudisca, Vanesa Romina; Simpson, Clare; Castelli, Lydia M.; Lui, Jennifer; Hoyle, Nathaniel P.; Moreno, Sílvia; Ashe, Mark P.; Portela, Paula

doi:10.1242/jcs.111534

Cited by 21 publications

(40 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To further decipher how the light pathway modulates PKA activity, we monitored PKA subcellular location, using GFP fusions of its catalytic subunits. In agreement with published data4748, PKA catalytic subunits concentrated in the nucleus in the absence of H 2 O 2 (Fig. 4f–h and Supplementary Fig.…”

Section: Resultssupporting

confidence: 93%

Light-sensing via hydrogen peroxide and a peroxiredoxin

et al. 2017

View full text Add to dashboard Cite

Yeast lacks dedicated photoreceptors; however, blue light still causes pronounced oscillations of the transcription factor Msn2 into and out of the nucleus. Here we show that this poorly understood phenomenon is initiated by a peroxisomal oxidase, which converts light into a hydrogen peroxide (H2O2) signal that is sensed by the peroxiredoxin Tsa1 and transduced to thioredoxin, to counteract PKA-dependent Msn2 phosphorylation. Upon H2O2, the nuclear retention of PKA catalytic subunits, which contributes to delayed Msn2 nuclear concentration, is antagonized in a Tsa1-dependent manner. Conversely, peroxiredoxin hyperoxidation interrupts the H2O2 signal and drives Msn2 oscillations by superimposing on PKA feedback regulation. Our data identify a mechanism by which light could be sensed in all cells lacking dedicated photoreceptors. In particular, the use of H2O2 as a second messenger in signalling is common to Msn2 oscillations and to light-induced entrainment of circadian rhythms and suggests conserved roles for peroxiredoxins in endogenous rhythms.

show abstract

Section: Resultssupporting

confidence: 93%

Light-sensing via hydrogen peroxide and a peroxiredoxin

et al. 2017

View full text Add to dashboard Cite

show abstract

“…After several hours of starvation, growth resumes with a distinct pattern of highly translated mRNAs, allowing enhanced synthesis of mitochondrial-targeted proteins (Vaidyanathan et al 2014). Altered protein kinase A signaling contributes to the translational reprograming (Ashe et al 2000;Tudisca et al 2012;Vaidyanathan et al 2014). Hence diverse cellular stress conditions can rapidly perturb global and mRNAspecific protein synthesis via multiple mechanisms to achieve stress-specific translational reprogramming.…”

Section: Global Approaches To Studying Translation Controlsmentioning

confidence: 99%

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

2016

View full text Add to dashboard Cite

In this review, we provide an overview of protein synthesis in the yeast Saccharomyces cerevisiae. The mechanism of protein synthesis is well conserved between yeast and other eukaryotes, and molecular genetic studies in budding yeast have provided critical insights into the fundamental process of translation as well as its regulation. The review focuses on the initiation and elongation phases of protein synthesis with descriptions of the roles of translation initiation and elongation factors that assist the ribosome in binding the messenger RNA (mRNA), selecting the start codon, and synthesizing the polypeptide. We also examine mechanisms of translational control highlighting the mRNA cap-binding proteins and the regulation of GCN4 and CPA1 mRNAs.

show abstract

“…Subcellular localization of PKA in S. cerevisiae depends on the environmental conditions such as carbon source and stress [21,23,54]. Several post-translational modifications on regulatory and catalytic subunits of yeast PKA affect its nuclear-cytoplasmic localization [55][56][57].…”

Section: Mechanism Of Nuclear Accumulation Of Pka In Response To Osmomentioning

confidence: 99%

“…In exponentially glucosegrowing cells, Bcy1 and Tpk2 localization is mainly nuclear, whereas Tpk1 and Tpk3 show a nucleus-cytoplasmic distribution [21,22]. Cell in stationary phase of growth or stressed cells show re-localization of all PKA subunits towards the cytoplasm, where Tpk2 and Tpk3, in particular, are accumulated in RNP (RiboNucleoProtein) granules [21,23].…”

Section: Introductionmentioning

confidence: 99%

PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae

Baccarini

Martínez-Montañés

Rossi

et al. 2015

Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanism

Self Cite

View full text Add to dashboard Cite

Gene expression regulation by intracellular stimulus-activated protein kinases is essential for cell adaptation to environmental changes. There are three PKA catalytic subunits in Saccharomyces cerevisiae: Tpk1, Tpk2, and Tpk3 and one regulatory subunit: Bcy1. Previously, it has been demonstrated that Tpk1 and Tpk2 are associated with coding regions and promoters of target genes in a carbon source and oxidative stress dependent manner. Here we studied five genes, ALD6, SED1, HSP42, RPS29B, and RPL1B whose expression is regulated by saline stress. We found that PKA catalytic and regulatory subunits are associated with both coding regions and promoters of the analyzed genes in a stress dependent manner. Tpk1 and Tpk2 recruitment was completely abolished in catalytic inactive mutants. BCY1 deletion changed the binding kinetic to chromatin of each Tpk isoform and this strain displayed a deregulated gene expression in response to osmotic stress. In addition, yeast mutants with high PKA activity exhibit sustained association to target genes of chromatin-remodeling complexes such as Snf2-catalytic subunit of the SWI/SNF complex and Arp8-component of INO80 complex, leading to upregulation of gene expression during osmotic stress. Tpk1 accumulation in the nucleus was stimulated upon osmotic stress, while the nuclear localization of Tpk2 and Bcy1 showed no change. We found that each PKA subunit is transported into the nucleus by a different β-karyopherin pathway. Moreover, β-karyopherin mutant strains abolished the chromatin association of Tpk1 or Tpk2, suggesting that nuclear localization of PKA catalytic subunits is required for its association to target genes and properly gene expression.

show abstract

PKA isoforms coordinate mRNA fate during nutrient starvation

Cited by 21 publications

References 48 publications

Light-sensing via hydrogen peroxide and a peroxiredoxin

Light-sensing via hydrogen peroxide and a peroxiredoxin

Mechanism and Regulation of Protein Synthesis in Saccharomyces cerevisiae

PKA-chromatin association at stress responsive target genes from Saccharomyces cerevisiae

Contact Info

Product

Resources

About