Placental extract protects bone marrow-derived stem/progenitor cells against radiation injury through anti-inflammatory activity

Abstract

Placental extracts have been reported to have anti-oxidative and anti-inflammatory activities. Because there is increasing evidence that ionizing radiation induces the release of reactive oxygen species (ROS) and inflammatory cytokines, we examined the protective effects of a placental extract against radiation injury. C57BL/6 mice were exposed to 1 Gy of γ-ray radiation every day for 5 days, and placental extract (1 mg/day) was administrated orally soon after each exposure. At 2 days after the last irradiation, mice were euthanized to examine the numbers, colony-forming capacity, and DNA damage of stem/progenitor cells in the peripheral blood and bone marrow. To understand the related mechanisms, we also measured the levels of intracellular and mitochondrial ROS, and 8-OHdG in the plasma and urine, and IL-6 and TNF-α in the plasma. Compared with the placebo treatment, oral administration of placental extract significantly increased the number and colony-forming capacity, but decreased the DNA damage of bone marrow stem/progenitor cells. However, neither the levels of intracellular and mitochondrial ROS in bone marrow cells, nor the levels of 8-OHdG in the urine and plasma significantly differed between groups. Interestingly, in comparison with the placebo treatment, placental extract significantly decreased the levels of the inflammatory cytokines IL-6 and TNF-α in the plasma. Placental extract significantly attenuated the acute radiation injury to bone marrow-derived stem/progenitor cells, and this protection is likely to be related to the anti-inflammatory activity of the placental extract.

Porcine placental extract facilitates memory and learning in aged mice

“…Moreover, the neuronal cells of the hippocampal CA3 region show progressive age‐associated decline (Bronzetti, Felici, Zaccheo, & Amenta, ; Brunson, Eghbal‐Ahmadi, Bender, Chen, & Baram, ; Hioki et al, ). It was expected that placental extract may reduce the loss of neuronal cells associated with age, as it is known to have cytoprotective effects (Kawakatsu, Urata, Goto, Ono, & Li, ; Kim et al, ; Liu et al, ). Porcine placental extract has previously been shown to suppress fear memory impairment and Nissl‐positive cell loss in the hippocampal CA3 region induced by restraint stress in ovariectomized mice (Takuma et al, ).…”

“…It was expected that placental extract may reduce the loss of neuronal cells associated with age, as it is known to have cytoprotective effects (Kawakatsu, Urata, Goto, Ono, & Li, 2012;Kim et al, 2010;Liu et al, 2019). Porcine placental extract has previously been shown to suppress fear memory impairment and Nissl-positive cell loss in the hippocampal CA3 region induced by restraint stress in ovariectomized mice (Takuma et al, 2012).…”

Putative Prophylaxes Updated of Placenta Extract and Aloe vera as Biogenic Stimulants

“…Interestingly, in comparison with the placebo treatment, PE significantly decreased the levels of the inflammatory cytokines IL-6 and TNF-α in the plasma. PE significantly attenuated the acute radiation injury to bone marrowderived stem/progenitor cells, and this protection is likely to be related to anti-inflammatory activity of the PE [40] .…”

Placental therapy: An insight to their biological and therapeutic properties

“…In was followed by administration of human placental extracts in Japan in the 1950s. Thereafter, placental extracts were used in aesthetics and therapeutics anti-aging strategies in countries such as India, Japan, Korea, Thailand and the United States of America [19][20][21][22][23].…”