Planar Waveguides as High-Performance Sensing Platforms for Fluorescence-Based Multiplexed Oligonucleotide Hybridization Assays

Budach, W.; Abel, Andreas P.; Bruno, and Alfredo E.; Neuschäfer, D.

doi:10.1021/ac990092e

Cited by 78 publications

(47 citation statements)

References 25 publications

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“…After spotting, the surface was incubated with BSA for passivation. 99 For the DNA microarrays, oligonucleotides were either spotted and covalently immobilized on silanized waveguides 80,97,100 or electrostatically attached by spotting a waveguide coated with positively charged poly(L-lysine)-graft-poly(ethylene glycol). 97 Recently, an oligonucleotide microarray using the IOW evanescent field detection system described above was presented for the identification of bacteria frequently responsible for septicemia.…”

Section: 79mentioning

confidence: 99%

Optical microarray biosensing techniques

Bally

Halter

Vörös

et al. 2006

Surface & Interface Analysis

161

126

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Microarrays provide a powerful analytical tool for the simultaneous analyses of thousands of parameters, be they DNA or proteins, in a single experiment. However, a number of challenges continue to hinder the widespread application of microarray assays for analytical and diagnostic purposes. Advances in detection methods can help overcome some of these challenges by improving sensitivity and reliability in signal detection and by enabling real-time detection of binding events. Thus, the aim of this review is to highlight the most promising optical microarray biosensing techniques. The principles of techniques making use of labels, including scanner type, total internal reflection type, fiber-optics-based, and SPRenhanced fluorescence, are described, along with a brief summary of labeling strategies. State of the art label-free techniques, including imaging SPR and imaging ellipsometry, are also reviewed. Examples of microarray-based assays using each technique are given to illustrate both their usefulness and their limits of detection. Furthermore, the most competitive commercial microarray systems are presented and compared with one another in the context of their detection system. Finally, a discussion of the remaining challenges as well as trends and future applications of microarrays are presented in the context of optical sensing.

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Section: 79mentioning

confidence: 99%

Optical microarray biosensing techniques

Bally

Halter

Vörös

et al. 2006

Surface & Interface Analysis

161

126

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“…After developing a grating-coupled waveguide method for exciting flurophores by TIRF [53], Budach demonstrated a 1-dimensional corrugated dielectric structure that functions as a wavelength-selective optical resonator that is capable of enhancing fluorescence intensity output by 52x [54] over a small surface area, using an expensive glass-based fabrication approach. The transducer made use of a uniformly corrugated surface made from a low refractive index glass material that is coated with a high refractive index material (e.g.…”

Section: Enhanced Fluorescence Detectionmentioning

confidence: 99%

Photonic crystals utilized for label-free and amplified fluorescence biodetection

Cunningham

2008

SPIE Proceedings

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Photonic crystals are fabricated on plastic surfaces, producing narrow bandwidth resonances at any desired wavelength. While shifts in the resonant wavelength quantify the density of adsorbed biomaterial, the resonances also enhance the output of adsorbed fluorophores. The combined attributes of photonic crystals enable highly sensitive label-free detection and greatly amplified sensitivity of any fluorescence-based assay for applications in life science research, drug discovery, and environmental pathogen detection. Applications for label-free selective detection of viral pathogens with sensitivity of ~30 focal forming units, and detection of spore pathogens with single-unit resolution are highlighted. Amplified fluorescence, meanwhile, enables gene expression detection of DNA at concentrations ~2 orders of magnitude lower than detection on optically passive surfaces.

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“…Based on TIR, disposable plastic prisms integrated on biochips [61] with several different types of evanescent waveguides, including fiber [62], channel [63], and planar [64,65] waveguides, have been proposed to address the requirements for ultra-sensitive, high-throughput platforms. In all of these systems, the excitation laser beam comes from the side of the chip, which requires highly precise aligned at a specified angle to achieve efficient light coupling and to create a strong evanescent field.…”

Section: Minaturized Total Internal Reflection (Mtir) Systemmentioning

confidence: 99%

Single Molecular Detection via Micro-Scale Polymeric Opto-Electro-Mechanical Systems

Lee¹,

Pisano²,

Pruitt³

et al. 2005

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Planar Waveguides as High-Performance Sensing Platforms for Fluorescence-Based Multiplexed Oligonucleotide Hybridization Assays

Cited by 78 publications

References 25 publications

Optical microarray biosensing techniques

Optical microarray biosensing techniques

Photonic crystals utilized for label-free and amplified fluorescence biodetection

Single Molecular Detection via Micro-Scale Polymeric Opto-Electro-Mechanical Systems

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