Plant-Based Nutraceutical Increases Plasma Catalase Activity in Healthy Participants: A Small Double-Blind, Randomized, Placebo-Controlled, Proof of Concept Trial

Abstract

Oxidative stress resulting from dietary, lifestyle and environmental factors is strongly associated with tissue damage and aging. It occurs when there is either an overproduction of reactive oxygen species (i.e., oxidants) or decreased bioavailability of antioxidants that can scavenge them. The objective of this 12-week double-blind placebo-controlled study was to assess the efficacy of a nutraceutical at augmenting antioxidant status. Healthy adults (25-45 y) were randomized to either a treatment group (Product B, n = 23) or a placebo group (control, n = 20). No significant effect of Product B was observed for anthropometric variables or markers of glucose and lipid regulation. Biomarkers of oxidative stress were likewise not altered following the 12-week intervention. Plasma catalase concentrations were significantly elevated following 12 weeks of Product B as compared to the control group (+6.1 vs. -10.3 nmol/min/mL, p = 0.038), whereas other measures of antioxidant capacity were not significantly different between the groups. Product B effectively augmented concentrations of the anti-aging antioxidant catalase in healthy adults.

<p>In silico and in vitro studies of lupeol and iso-orientin as potential antidiabetic agents in a rat model</p>

“…Certain stress markers were measured to evaluate oxidative stress. Malondialdehyde (MDA) was estimated in terms of thiobarbituric acid reactive substances with the help of spectrophotometric method as described by Ohkawa et al14 Superoxide dismutase (SOD) was estimated with the help of another spectrophotometric method described by Kakkar et al15 Glutathione (GSH), glutathione peroxidase (GPx), glutathione reductase (GRx), catalase (CAT), and nitric oxide (NO) were estimated by their respective spectrophotometric methods as explained by Moron et al,16 Sweazea et al,17 and Bredt and Synder 18…”

Antioxidant effects of calligonum extract on ovarian tissue of PCO model: An experimental study

“…The balance in reactive oxygen species (ROS) plays an important physiological role in several processes of the female reproductive system, including oocyte maturation, ovulation, fertilization, and endometrial loss (3). Anti-oxidative enzymes are include catalase, superoxide dismutase and glutathione peroxidase that they have protective mechanisms against the oxidative stress effects (4). These factors contribute to the balanced production of ROS by cells in the body (5).…”

Relative Bioavailability of Silybin A and Silybin B From 2 Multiconstituent Dietary Supplement Formulations Containing Milk Thistle Extract: A Single-dose Study

“…The Product B formulation was recently reported to significantly increase plasma catalase concentrations compared to placebo treatment in healthy volunteers. 7 Subsequently, IsaGenesis, a newly-reformulated version was developed as a softgel with fat-soluble components postulated to provide better bioavailability compared to the dry powdered Product B formulation. Because milk thistle extracts were by far the most abundant component in both formulations, and since silybin A and silybin B are found in much higher concentrations in the systemic circulation relative to the amounts of the other flavonolignans (e.g.…”