Plant Cell Culture

Evans, D. E.; Coleman, J. O. D.; Kearns, Anne

doi:10.1201/9781003076940

Cited by 11 publications

(8 citation statements)

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“…Kalus remah dan Hasil penelitian Fatmawati (2008) menyebutkan bahwa warna kalus menginduksi keberadaan klorofil dalam jaringan, semakin hijau warna kalus semakin banyak pula kandungan klorofilnya. Terjadinya perbedaan warna kalus disebabkan adanya perubahan pigmentasi yang dipengaruhi oleh nutrisi dan faktor lingkungan (Evans et al, 2020) selain itu, pencahayaan berperan dalam pembentukan warna kalus. George (1993), menyatakan bahwa cahaya putih dapat merangsang pembentukan kalus dan organogenesis dalam kultur jaringan.…”

Section: Morfologi Kalusunclassified

Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang

2022

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The purpose of the study is to find the best Benzil Amino Purine (BAP) concentrations and proper explants for the growth of the apple cucumber callus (Cucumis sp.) on Murashige and Skoog (MS) media. The research method used was an experimental method with nonparametric statistics with treatments A0B1 (BAP 0 + Leaves), A0B2 (BAP 0 + Stem), A1B1 (BAP 0.5 μM + Leaf), A1B2 (BAP 0.5 μM + Stem), A2B1 (BAP 1.5 μM + Leaf), A2B2 (BAP 1.5 μM + Stem), A3B1 (BAP 3.0 μM+ Leaves) and A3B2 (BAP 3.0 μM+ Stems) and analyzed descriptively using the Kruskal Wallis Test. The parameters observed were callus emergence, callus diameter, growth rate and callus morphology. The results showed accretion of an apple cucumber callus at concentration 0.5 μM BAP + stem which exerted a faster effect on callus formation time (27 hsi), 1.5 μM BAP + stem giving the largest diameter with 1.2 cm. The stem explant gives the best growth rate (0.8 cm/3 day) as well as concentrations of 0.5 μM and 1.5 μM giving the best growth rate (0.462 cm/3 day and 0.887 cm/3 day). This result showed calluses A0B2 and A1B1 (compact + green) as well as A1B2 and A2B2 (crumb + yellowish-green).

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Section: Morfologi Kalusunclassified

Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang

2022

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“…This is followed by mass propagation of the callus to obtain sufficient biomass for initiating a suspension culture using shaking flasks. Here it is important to work with a friable callus [54]. An efficient production process in the bioreactor requires a homogeneous, well‐growing and productive suspension culture, which sequentially requires a homogenization procedure [55] for the suspension culture, performed over several weeks.…”

Section: Commercial Food Ingredients Based On Plant Cell Culture Techmentioning

confidence: 99%

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

Gubser

Vollenweider

Eibl

et al. 2021

Engineering in Life Sciences

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Climate change and an increasing world population means traditional farming methods may not be able to meet the anticipated growth in food demands. Therefore, alternative agricultural strategies should be considered. Here, plant cell and tissue cultures (PCTCs) may present a possible solution, as they allow for controlled, closed and sustainable manufacturing of extracts which have been or are still being used as colorants or health food ingredients today. In this review we would like to highlight developments and the latest trends concerning commercial PCTC extracts and their use as food ingredients or even as food. The commercialization of PCTC‐derived products, however, requires not only regulatory approval, but also outstanding product properties or/and a high product titer. If these challenges can be met, PCTCs will become increasingly important for the food sector in coming years.

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“…For example, if shoots emerge it may be cultured freshly with auxin to produce plantlets which, if plotted in potting soil can grow further as normal plants. 23 Important steps before starting tissue Culture Process This section of the review will focus on the desirable steps required before starting plant tissue culture: The first and basic steps of the culture are the requirement of nutritive media which can be liquid and solid based on the outcome uses.…”

Section: Plant Tissue Culture Processmentioning

confidence: 99%

Trends in the Use of Tissue Culture, Applications and Future Aspects

Twaij

Jazar

Hasan

2020

IJPB

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Plant tissue culture has developed widely incorporated into biotechnology, the agricultural systems being a key factor to support many pharmaceutical and industrial outcomes. Since 1902 there is vast progress in plant culture and its application has emerged having great diversity in the science filed. Due to development and desire to grow on high scale production in the past few decades, tissue culture techniques were manipulated for improvement of plant growth, biological activities, transformation, and secondary metabolites production. A significant advance in techniques has been sought to deal with problems of low concentrations of secondary metabolites in whole plants. The augmented use of plant culture is due to a superior perceptive of plant oriented compounds and secondary metabolites from economically important plants. Due to development in modern techniques, several particular protocols have been developed for the production of a wide array of secondary metabolites of plants on a commercial scale. Plant tissue culture has to lead to significant contributions in recent times and today they constitute an indispensable tool in the advancement of agricultural sciences and modern agriculture. This review would enable us to have an analysis of plant tissue culture development for agriculture, human health and wellbeing in general.

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Plant Cell Culture

Cited by 11 publications

References 0 publications

Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang

Various Concentration Distribution of Benzil Amino Purine Concentration on Callus Growth in Apple Cucumber Originated from Karawang

Food ingredients and food made with plant cell and tissue cultures: State‐of‐the art and future trends

Trends in the Use of Tissue Culture, Applications and Future Aspects

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