Plant Cell Cultures as a Tool to Study Programmed Cell Death

Malerba, Massimo

doi:10.3390/ijms22042166

Cited by 18 publications

(11 citation statements)

References 54 publications

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“…In order to assess the dependence of cAMP on the phosphoproteome, we compared WT and cAS TBY cell lines grown for 3 days at 27 • C. Plant cell suspensions were chosen since they are a cytologically uniform and reproducible system that is suitable for an efficient induction of changes in the physical environment [49]. In the mutant line, the cAMP content was reduced by approximately 50% due to the sequestration by the sponge which is based on the a PKA I regulatory subunit that specifically binds free cAMP [6].…”

Section: Differentially Abundant Phosphosites (Dapps) In Response To ...mentioning

confidence: 99%

Quantitative phosphoproteomics reveals novel roles of cAMP in plants

Domingo

Marsoni

Chiodaroli³

et al. 2023

Proteomics

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3′,5′-cyclic adenosine monophosphate (cAMP) is finally recognized as an essential signaling molecule in plants where cAMP-dependent processes include responses to hormones and environmental stimuli. To better understand the role of 3′,5′-cAMP at the systems level, we have undertaken a phosphoproteomic analysis to elucidate the cAMP-dependent response of tobacco BY-2 cells. These cells overexpress a molecular "sponge" that buffers free intracellular cAMP level. The results show that, firstly, in vivo cAMP dampening profoundly affects the plant kinome and notably mitogen-activated protein kinases, receptor-like kinases, and calcium-dependent protein kinases, thereby modulating the cellular responses at the systems level. Secondly, buffering cAMP levels also affects mRNA processing through the modulation of the phosphorylation status of several RNA-binding proteins with roles in splicing, including many serine and arginine-rich proteins. Thirdly, cAMP-dependent phosphorylation targets appear to be conserved among plant species. Taken together, these findings are consistent with an ancient role of cAMP in mRNA processing and cellular programming and suggest that unperturbed cellular cAMP levels are essential for cellular homeostasis and signaling in plant cells.

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Section: Differentially Abundant Phosphosites (Dapps) In Response To ...mentioning

confidence: 99%

Quantitative phosphoproteomics reveals novel roles of cAMP in plants

Domingo

Marsoni

Chiodaroli³

et al. 2023

Proteomics

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“…Cell cultures are maintained under aseptic controlled conditions [ 19 ]. This allows a high reproducibility of results to be achieved and the use cell cultures as model systems for studying many processes, like cell specialization [ 20 ], organogenesis [ 21 ], embryogenesis [ 22 ], reproductive processes, cellular responses to endogenous [ 20 , 23 , 24 ] and exogenous factors [ 25 , 26 , 27 ], programmed cell death [ 28 ], the metabolome [ 29 ], etc. In addition, due to their advantages, cell cultures have found wide application in biotechnology [ 17 , 30 , 31 , 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

Plant Heterotrophic Cultures: No Food, No Growth

Puzanskiy,

Romanyuk,

Kirpichnikova

et al. 2024

Plants

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Plant cells are capable of uptaking exogenous organic substances. This inherited trait allows the development of heterotrophic cell cultures in various plants. The most common of them are Nicotiana tabacum and Arabidopsis thaliana. Plant cells are widely used in academic studies and as factories for valuable substance production. The repertoire of compounds supporting the heterotrophic growth of plant cells is limited. The best growth of cultures is ensured by oligosaccharides and their cleavage products. Primarily, these are sucrose, raffinose, glucose and fructose. Other molecules such as glycerol, carbonic acids, starch, and mannitol have the ability to support growth occasionally, or in combination with another substrate. Culture growth is accompanied by processes of specialization, such as elongation growth. This determines the pattern of the carbon budget. Culture ageing is closely linked to substrate depletion, changes in medium composition, and cell physiological rearrangements. A lack of substrate leads to starvation, which results in a decrease in physiological activity and the mobilization of resources, and finally in the loss of viability. The cause of the instability of cultivated cells may be the non-optimal metabolism under cultural conditions or the insufficiency of internal regulation.

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“…We accomplished that in this study by using Arabidopsis thaliana cell suspension culture (ACSC), a widely used model for studying plant PCD (Malerba and Cerana, 2021). The ACSC consists of a homogenous cell population in which cell death can easily be induced by a variety of chemical or physical stimuli (McCabe and Leaver, 2000; Lanubile et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis cell suspension culture and RNA sequencing reveal regulatory networks underlying plant programmed cell death

Burke

McCabe

Sonawane

et al. 2023

Preprint

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Programmed cell death (PCD) facilitates targeted elimination of redundant, damaged, or infected cells via genetically controlled pathways. In plants, PCD is often an essential component of normal development and can also mediate responses to abiotic and biotic stress stimuli. However, studying the transcriptional regulation of this fundamental process is hindered by difficulties in sampling small groups of cells undergoing PCD that are often buried within the bulk of living plant tissue. We addressed this challenge by using RNA sequencing (RNA-Seq) of Arabidopsis thaliana suspension cells, a system that allows precise monitoring of PCD activation and progression. The use of three PCD-inducing treatments (salicylic acid, heat and critical dilution), in combination with three cell death modulators (3-methyladenine, lanthanum chloride and conditioned medium), allowed isolation of candidate core and stimuli-specific PCD genes, inference of underlying gene regulatory networks and identification of putative transcriptional regulators. This analysis underscored cell cycle disturbance and the repression of both pro-survival stress responses and mitochondrial retrograde signalling as key elements of the PCD-associated transcriptional signature in plants. Further, phenotyping of twenty Arabidopsis T-DNA insertion mutants in selected candidate genes confirmed a role for several in PCD and stress tolerance regulation, and validated the potential of these generated resources to identify novel genes involved in plant PCD pathways and/or stress tolerance in plants.

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Plant Cell Cultures as a Tool to Study Programmed Cell Death

Cited by 18 publications

References 54 publications

Quantitative phosphoproteomics reveals novel roles of cAMP in plants

Quantitative phosphoproteomics reveals novel roles of cAMP in plants

Plant Heterotrophic Cultures: No Food, No Growth

Arabidopsis cell suspension culture and RNA sequencing reveal regulatory networks underlying plant programmed cell death

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