Plant DNA polymerases

Litvak, Simón; Castroviejo, Michel

doi:10.1007/bf02418250

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…DNA topoisomerase III α, endonuclease MUS81 and phosphatidylinositol kinase are associated with DNA topology, stalled replication forks or signal transduction 28 - 30 . OsDSHCT was shown to interact with proteins implicated in RNA degradation and RNA-binding (Rrp6, NIFK), as well as with DNA polymerase σ, playing essential roles in chromatin remodeling and DNA repair 31 and the E3 ubiquitin ligase involved in polyubiquitination and post-translational regulation 32 …”

Section: Resultsmentioning

confidence: 99%

Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

Macovei

Tuteja²

2013

Plant Signaling & Behavior

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Ionizing radiation currently represents an important tool to generate genetic variability that does not exist in nature, especially in plants. Even so, the radiological protection still represents a subject of regulatory concern. In plants, few reports dealing with the effects of γ-rays, in terms of dose rate (rate of energy deposition) and total dose (energy absorbed per unit mass), are available. In addition, plants are known to be more radioresistant than animals. The use of ionizing radiations for studying various aspects of transcription regulation may help elucidate some of the unanswered questions regarding DNA repair in plants. Under these premises, microRNAs have emerged as molecules involved in gene regulation in response to various environmental conditions as well as in other aspects of plant development. Currently, no report on the changes in microRNAs expression patterns in response to γ-ray treatments exists in plants, even if this subject is extensively studies in human cells. The present study deals with the expression profiles of three miRNAs, namely osa-miR414, osa-miR164e and osa-miR408 and their targeted helicase genes (OsABP, OsDBH and OsDSHCT) in response to different doses of γ-rays delivered both at low and high dose rates. The irradiated rice seeds were grown both in the presence of water and 100 mM NaCl solution. DNA damage and reactive species accumulation were registered, but no dose- or time-dependent expression was observed in response to these treatments.

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Section: Resultsmentioning

confidence: 99%

Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

Macovei

Tuteja²

2013

Plant Signaling & Behavior

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“…Nevertheless, different types of plant DNA polymerases have been purified and characterized [3,4,7,8,13,20,25,30].…”

Section: Introductionmentioning

confidence: 99%

Changes of enzymes and factors involved in DNA synthesis during wheat embryo germination

Benedetto

Ech-Chaoui²,

Plissonneau

et al. 1996

Plant Mol Biol

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We have previously purified and characterized wheat germ DNA polymerases A and B. To determine the role played by DNA polymerases A and B in DNA replication, we have measured the level of their activities during wheat embryo germination. The level of cellular proteins known to be associated with DNA synthesis such as PCNA and DNA primase were also investigated. The activity of DNA polymerase A gradually increased reaching a maximal level at 12 h after germination. Three days later, only a residual activity was detected. DNA polymerase B showed the same pattern during germination with very similar changes in activity. Our results indicate a striking correlation between maximal activities of DNA polymerase A, DNA polymerase B and optimal levels of DNA synthesis. These results support a replicative role of these enzymes. The activity of wheat DNA primase that copurifies with DNA polymerase A also increases during wheat germination. Taking together all its properties, and in spite of its behaviour with some inhibitors. DNA polymerase A may be considered as the plant counterpart of animal DNA polymerase alpha. Concerning DNA polymerase B we have previously shown that PCNA stimulates its processivity. Besides studying the changes of DNA polymerases A and B and DNA primase we have also studied changes in PCNA during germination. We show that PCNA is present in wheat embryos at a constant relatively high level during the first 24 h of germination. After 48 h, the absence of PCNA is concomitant with an important decrease in DNA polymerase B activity. In this report we confirm the behaviour of DNA polymerase B as a delta-like activity.

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“…2), which was almost equivalent to that in tobacco leaf protoplasts (data not shown). Over 50% of the incorporation was blocked by addition of aphidicolin, a potent inhibitor of DNA replication [13,17,22]. These data suggest that if maize protoplasts are maintained in culture they will proceed from G1 to S phase.…”

Section: Dna Synthesis In Maize Leaf Protoplastsmentioning

confidence: 90%

“…(3) Effect of aphidicolin: aphidicolin is a specific inhibitor of DNA polymerase-~t [13,17,22] and thus inhibits DNA replication. To test whether nuclear labelling was due to replicative DNA synthesis, aphidicolin was added to the culture medium at 10mg1-1 from a stock solution of 10mgml -~ in dimethyl sulfoxide (DMSO).…”

Section: Effects Of Various Media Additives On Dna Synthesismentioning

confidence: 99%

DNA replication in maize leaf protoplasts

Wang

Cutler

Saleem

et al. 1989

Plant Cell Tiss Organ Cult

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Maize leaf protoplasts were investigated for their metabolic competence and capacity to synthesize DNA. When protoplasts were incubated at elevated temperatures, they exhibited a heat shock response with specific proteins being preferentially synthesized. This indicated that the protoplasts were fully metabolically functional and capable of responding to environmental stimuli. Significant DNA synthesis was observed in these protoplasts after incorporation of 3H-thymidine into chromatin by trichloroacetic acid precipitation and by incorporation of 5-bromo-2-deoxyuridine (BrdU), an analog of thymidine, detected by immunofluorescence. The immunocytochemical method revealed that about 50% of nuclei in the maize leaf protoplasts were labelled after 3 days of culture and that most of these nuclei were labelled as intensely as normal mitotic cells. Aphidicolin, an inhibitor of DNA polymerase-a, decreased the percentage of labelled nuclei, demonstrating that the labelling was substantially due to replicative DNA synthesis. However, chromosome condensation was not observed. It is proposed that these protoplasts are capable of DNA synthesis, but incapable of nuclear division. Effects of media additives on the number of nuclei entering S phase in these protoplasts were also assessed by the immunocytocbemical method. Inclusion of 80 mM Ca 2+ in the enzyme solution increased protoplast yield and also appeared beneficial to DNA synthesis. The antioxidant, n-propyl gallate, which was used to stabilize the protoplasts, delayed the onset of DNA synthesis. Arginine and spermidine produced a slight increase in DNA synthesis.Abbreviations: BrdU--5-bromo-2-deoxyuridine; DMSO--dimethyl sulfoxide; n-PG--npropyl gallate; PBS--phosphate-buffered saline

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Plant DNA polymerases

Cited by 14 publications

References 29 publications

Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

Different expression of miRNAs targeting helicases in rice in response to low and high dose rate γ-ray treatments

Changes of enzymes and factors involved in DNA synthesis during wheat embryo germination

DNA replication in maize leaf protoplasts

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