Plant Husbandry and Management Techniques for Growing Grain Legumes Under Simulated Tropical Conditions in Controlled Environments

Summerfield, R. J.; Huxley, P. A.; Minchin, F. R.

doi:10.1017/s0014479700007638

Cited by 76 publications

(58 citation statements)

References 8 publications

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“…Jars were previously sterilized by autoclaving at 121 o C for 1 hour, and then 200 cm 3 of nutrient solution (Summerfield et al, 1977) was added. The nutrient solution was prepared in a disinfected container using deionized water.…”

Section: Methodsmentioning

confidence: 99%

Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii

Ramos

Parsons

Sprent

2005

Pesq. agropec. bras.

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Pesq. agropec. bras., Brasília, v.40, n.5, p.453-458, maio 2005 Ureide and amino acid content of water stressed soybean 453Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii Maria Lucrecia Gerosa Ramos (1) , Richard Parsons (2) and Janet Irene Sprent , and asparagine was not detected. Nodules formed by the strain 29W accumulated 1 µmol g -1 of aspartate, whether plants were stressed or not. Asparagine was the major amino acid found in nodules from watered plants (6 µmol g -1) and the amount of this amino acid was six-fold increased when plants were water stressed.Index terms: Glycine max, alanine, asparagine, aspartate, glutamate, nitrogen fixation. Alterações na concentração de ureídos e aminoácidos em soja sob estresse hídrico com inoculação de Bradyrhizobium japonicum e B. elkaniiResumo -O objetivo deste trabalho foi estudar a resposta da soja, com inoculação de Bradyrhizobium japonicum (estirpe CB1809, Semia 586) ou B. elkanii (estirpe 29W, Semia 5019), ao estresse hídrico. Plantas com inoculação da estirpe CB1809 produziram maior fração de raízes (19%) do que aquelas com inoculação de 29W (14,6%). As plantas com inoculação de CB1809 produziram menos nódulos e acumularam mais nitrogênio do que aquelas com inoculação de 29W. Em geral, baixos teores de ureídos nos nódulos foram encontrados em plantas irrigadas, com inoculação de CB1809 ou 29W, mas esses valores aumentaram cinco vezes em plantas com CB1809, sob estresse hídrico. Os nódulos formados pela estirpe CB1809 produziram, principalmente, aspartato e glutamato, ao passo que aqueles formados pela estirpe 29W produziram mais glutamato, asparagina e alanina. Nos nódulos de plantas com inoculação de CB1809, o aspartato foi o aminoácido que apresentou maior acumulação (5 µmol g -1 ); em plantas sob estresse hídrico, esse aminoácido alcançou 26 µmol g -1; e não foi detectada asparagina. Os nódulos formados pela estirpe 29W acumularam 1 µmol g -1 de aspartato, em plantas com ou sem estresse. A asparagina foi o principal aminoácido encontrado nos nódulos de plantas irrigadas (6 µmol g -1 ); a quantidade desse aminoácido foi aumentada em seis vezes, quando as plantas foram submetidas ao estresse hídrico.Termos para indexação: Glycine max, alanina, asparagina, aspartato, fixação de nitrogênio, glutamato.

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Section: Methodsmentioning

confidence: 99%

Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii

Ramos

Parsons

Sprent

2005

Pesq. agropec. bras.

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“…The macronutrient solution composition (Summerfield et al, 1977) Plants were kept at field capacity for 24 days before the stress treatments were started. Water was withheld from half of the plants which were harvested after five and eight days of water stress.…”

Section: Methodsmentioning

confidence: 99%

Effect of water stress on nitrogen fixation and nodule structure of common bean

Ramos

Parsons

Sprent

et al. 2003

Pesq. agropec. bras.

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-The aim of this work was to investigate the effect of water stress on N 2 fixation and nodule structure of two common bean (Phaseolus vulgaris L.) cultivars Carioca and EMGOPA-201. Plants were harvested after five and eight days of water stress. Carioca had lower nodule dry weight on both water stress periods; shoot dry weight was lower at five days water stress and did not differ from control after eight days stress. Both cultivars had lower nitrogenase activity than control after five and eight days water stress. For both cultivars, after eight days stress bacteroid membranes were damaged. Carioca presented more pronounced damage to infected tissue, with host cell vacuolation and loss of the peribacteroid membrane at five days after stress; at eight days after stress, there was degradation of cytoplasm host cells and senescence of bacteroids, with their release into intercellular spaces. Intensity of immunogold-labeling of intercellular cortical glycoprotein with the monoclonal antibodies MAC 236/ 265 was different for both cultivars.Index terms: Phaseolus vulgaris, nitrogenase, leaf water potential, nodulation. Efeito do estresse hídrico na fixação biológica de nitrogênio e estrutura de nódulos de feijãoResumo -O objetivo deste trabalho foi investigar o efeito do estresse hídrico na fixação biológica do N 2 e na estrutura dos nódulos de feijão (Phaseolus vulgaris L.), nas cultivares Carioca e EMGOPA-201. As plantas foram colhidas após cinco e oito dias de estresse hídrico. A cultivar Carioca apresentou menor peso seco de nódulos que o controle (plantas irrigadas). O peso da parte aérea seca da cultivar Carioca foi menor aos cinco dias de estresse hídrico e não diferiu do controle, após oito dias de estresse. As duas cultivares tiveram menor atividade da nitrogenase após cinco e oito dias de estresse hídrico e apresentaram danos na membrana peribacteróide após oito dias de estresse. A cultivar Carioca foi mais afetada do que a EMGOPA-201, com vacuolação das células e perda da membrana peribacteróide após cinco dias de estresse; aos oito dias, houve degradação das células do citoplasma, senescência e liberação dos bacteróides nos espaços intercelulares. A intensidade de glicoproteínas nos espaços intercelulares do córtex, marcada com os anticorpos MAC 236/265, foi diferente nas duas cultivares de feijão.Termos para indexação: Phaseolus vulgaris, nitrogenase, potencial de água na folha, nodulação.

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“…Surface-sterilized seeds were germinated at 30°C for 3 d over sterile wet filter paper and then planted in pots with vermiculite or coarse quartz sand. Pots were watered 3 d per week with the plant nutrient solution reported by Summerfield et al (1977). For 2P conditions, K 2 HPO 4 concentration of the plant nutrient solution was reduced from 1 mM to 5 mM.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

et al. 2007

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Phosphorus (P) is an essential element for plant growth. Crop production of common bean (Phaseolus vulgaris), the most important legume for human consumption, is often limited by low P in the soil. Functional genomics were used to investigate global gene expression and metabolic responses of bean plants grown under P-deficient and P-sufficient conditions. P-deficient plants showed enhanced root to shoot ratio accompanied by reduced leaf area and net photosynthesis rates. Transcript profiling was performed through hybridization of nylon filter arrays spotted with cDNAs of 2,212 unigenes from a P deficiency root cDNA library. A total of 126 genes, representing different functional categories, showed significant differential expression in response to P: 62% of these were induced in P-deficient roots. A set of 372 bean transcription factor (TF) genes, coding for proteins with Inter-Pro domains characteristic or diagnostic for TF, were identified from The Institute of Genomic Research/Dana Farber Cancer Institute Common Bean Gene Index. Using real-time reverse transcription-polymerase chain reaction analysis, 17 TF genes were differentially expressed in P-deficient roots; four TF genes, including MYB TFs, were induced. Nonbiased metabolite profiling was used to assess the degree to which changes in gene expression in P-deficient roots affect overall metabolism. Stress-related metabolites such as polyols accumulated in P-deficient roots as well as sugars, which are known to be essential for P stress gene induction. Candidate genes have been identified that may contribute to root adaptation to P deficiency and be useful for improvement of common bean.

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Plant Husbandry and Management Techniques for Growing Grain Legumes Under Simulated Tropical Conditions in Controlled Environments

Cited by 76 publications

References 8 publications

Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii

Differences in ureide and amino acid content of water stressed soybean inoculated with Bradyrhizobium japonicum and B. elkanii

Effect of water stress on nitrogen fixation and nodule structure of common bean

Phosphorus Stress in Common Bean: Root Transcript and Metabolic Responses

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