Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Xiao, Qi; Mu, Xinlu; Liu, Jiushi; Li, Bin; Liu, Haitao; Zhang, Bengang; Xiao, Pei‐Gen

doi:10.1186/s13020-022-00601-y

Cited by 54 publications

(27 citation statements)

References 99 publications

(72 reference statements)

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“…Chromatography-based untargeted metabolomics analyses are rapid and reliable approaches which have been quickly developed and applied for the quality research of Chinese herbal medicines in recent years (Tao et al, 2018;Lu, et al, 2022;Xiao, et al, 2022). However, appropriate screening and Frontiers in Pharmacology frontiersin.org selection of the chemical markers with minimum combination for effective species discrimination is very important for such studies.…”

Section: Species Discrimination Of Bch and Nch With Metabolomic-based...mentioning

confidence: 99%

Comprehensive comparison on the anti-inflammation and GC-MS-based metabolomics discrimination between Bupleuri chinense DC. and B. scorzonerifolium Willd

et al. 2022

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Bupleuri Radix (BR) is a traditional Chinese medicine and widely used for cold and fever, influenza, inflammation, hepatitis and menstrual diseases. Two authentic medicinal plants of Bupleuri chinense DC. (Beichaihu, BCH) and B. scorzonerifolium Willd. (Nanchiahu, NCH) are recommended by the current Chinese Pharmacopoeia for BR. In the present study, the comparative investigations on the anti-inflammatory effects and gas chromatography-mass spectrometry (GC-MS)-based metabolomics for the species discrimination of BCH and NCH were conducted and reported. The in vitro evaluations indicated that the supercritical fluid extracts (SFEs) (IC50 of 6.39 ± 0.52 and 1.32 ± 0.05 mg (herb)/mL for BCH and NCH) were determined to be more potent than those of the hydro-distillation extracts (HDEs) (IC50 of 203.90 ± 8.08 and 32.32 ± 2.27 mg (herb)/mL for BCH and NCH) against LPS-induced inflammation in RAW264.7 macrophages. The higher anti-inflammatory effects of NCH were associated to its different chemical compositions to the BCH as characterized by the GC-MS analysis. Furthermore, based on the metabolomics and deep chemometric approaches, a minimum combination containing 15 chemical markers was optimized from the identified components and successfully applied for the species discrimination of BCH and NCH. This study not only helps to comparative understand BCH and NCH both in phytochemistry and pharmacology, but also provides the potential chemical markers for improvement of methods for the quality control of BCH and NCH.

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Section: Species Discrimination Of Bch and Nch With Metabolomic-based...mentioning

confidence: 99%

Comprehensive comparison on the anti-inflammation and GC-MS-based metabolomics discrimination between Bupleuri chinense DC. and B. scorzonerifolium Willd

et al. 2022

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“…The HPLC and MS results of the isolated active ingredients were compared with those reported in the existing literature. The ten isolated compounds were identified as wogonin, ononin, isoquercitrin, calycosin‐7‐glucoside, 3‐hydroxy‐9,10‐dimethoxyptercarpan, hyperoside, 7,2′‐dihydroxy‐3′,4′‐dimethoxyisoflavan, baicalein, calycosin, and soyasaponin (corresponding to peaks 1–10 in the HPLC diagram, respectively) [24] (Figure 5). According to the area normalization method, their purities were 98.53%, 96.72%, 96.11%, 95.29%, 97.91%, 98.25%, 95.46%, 95.23%, 95.18%, and 95.57%, respectively.…”

Section: Resultsmentioning

confidence: 99%

Extraction and preparation of 5‐lipoxygenase and acetylcholinesterase inhibitors from Astragalus membranaceus stems and leaves

Liu

Zhang

et al. 2023

J of Separation Science

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In this study, an efficient method that employs 5‐lipoxygenase and acetylcholinesterase as biological target molecules in receptor–ligand affinity ultrafiltration–liquid chromatography was developed for the screening of enzyme inhibitors derived from the Astragalus membranaceus stems and leaves. The effects of the extraction time, number of extraction cycles, ethanol concentration, and liquid–solid ratio on the total yield of the target compounds were investigated using response surface methodology, and the bioactive components were isolated using a combination of semi‐preparative high‐performance liquid chromatography and high‐speed countercurrent chromatography via a two‐phase solvent system consisting of n‐hexane–ethyl acetate–methanol–water (1:6:2:6, v/v/v/v). Subsequently, 10 naturally‐occurring bioactive components in the Astragalus membranaceus stems and leaves, including wogonin, ononin, isoquercitrin, calycosin‐7‐glucoside, 3‐hydroxy‐9,10‐dimethoxyptercarpan, hyperoside, 7,2′‐dihydroxy‐3′,4′‐dimethoxyisoflavan, baicalein, calycosin, and soyasaponin, were screened using affinity ultrafiltration to determine their potential effects against Alzheimer's disease. Consequently, all target compounds had purities higher than 95.0%, and the potential anti‐Alzheimer's disease effect of the obtained bioactive compounds was verified using molecular docking analysis. Based on the results, the back‐to‐back screening of complex enzyme inhibitors and separation of the target bioactive compounds using complex chromatography could provide a new approach to the discovery and preparation of natural active ingredients.

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“…The quality of herbal materials is well-known to be affected by factors such as origin, harvesting time, cultivation sites, post-harvesting processing, procedures in extraction and preparation, and others ( 61 ). Quality consistency evaluation becomes necessary for quality assurance of finished HCE products.…”

Section: Discussionmentioning

confidence: 99%

Chemical composition and anti-aging effects of standardized herbal chicken essence on D-galactose- induced senescent mice

Tung

Yen

et al. 2022

Front. Nutr.

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This study aimed to examine the chemical and anti-aging properties of chicken essence (CE) prepared with Sesamum indicum, Angelica acutiloba, and Zingiber officinale (HCE). HCE was analyzed for nutritional and phytochemical composition, and its anti-aging effects were investigated on the D-galactose (Gal)-induced aging mice. Results showed that HCE possessed significantly higher calories and contents of valine and total phenols than CE; it also contained significant amounts of ferulic acid, sesamin, and sesamolin. HCE significantly decreased MDA and NO levels in serum and liver and increased liver GSH levels in the D-Gal-induced mice. HCE greatly enhanced SOD and CAT activities in serum and liver, and liver GPx activity, as well as upregulating SIRT1 expression and downregulating TNF-α, IL-1β, IL-6, iNOS, Cox-2, and MCP-1 expression in liver tissues. This study demonstrates that HCE was effective in suppressing the aging process through enhancing antioxidant and anti-inflammatory activities and modulating the aging-related gene expression.

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Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Cited by 54 publications

References 99 publications

Comprehensive comparison on the anti-inflammation and GC-MS-based metabolomics discrimination between Bupleuri chinense DC. and B. scorzonerifolium Willd

Comprehensive comparison on the anti-inflammation and GC-MS-based metabolomics discrimination between Bupleuri chinense DC. and B. scorzonerifolium Willd

Extraction and preparation of 5‐lipoxygenase and acetylcholinesterase inhibitors from Astragalus membranaceus stems and leaves

Chemical composition and anti-aging effects of standardized herbal chicken essence on D-galactose- induced senescent mice

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