Plant proteomics update (2007–2008): Second-generation proteomic techniques, an appropriate experimental design, and data analysis to fulfill MIAPE standards, increase plant proteome coverage and expand biological knowledge

Post-Genomics Studies of Developmental Processes in Legume Seeds

“…Despite some efforts to identify metabolic/ expression profiles in cells of differing cell types, most data is the sum of values for different cell types in one organ or tissue. The definition and application of a series of -omics standards (Field and Sansone, 2006;Taylor et al, 2008;Jorrín-Novo et al, 2009) should overcome the problem of assembling/comparing datasets produced under diverse experimental conditions.…”

A Review beyond the borders: Proteomics of microclonial black fungi and black yeasts

“…The methodology shows its power to separate a high number of proteins and visualize the alteration of protein patterns [11,12,30, Figure 2]. One-dimensional electrophoresis has been used in combination with mass spectrometry and despite its simplicity for the separation of the fungal proteome, leading to the discovery of novel proteins like malate dehydrogenase and peptydil prolyl cis-trtans isomerase [31,32] However, SDS-PAGE remains a qualitative methodology and the separation of the proteins in the bands is limited, resulting in a heterogenous band containig different proteins of identical molecular weight.…”

Proteomic Analyses of Cells Isolated by Laser Microdissection

“…The use of proteomics in plant biology research has increased significantly over the last few years with an improvement in both quality and quantitative analysis, inaugurating a new phase of "Second Generation Plant Proteomics" (Jorrín et al, 2009). This growing interest in plant proteomics has continually produced a large number of developmental studies on plant cell division, elongation, differentiation, and formation of various organs using various proteomics approaches (Hochholdinger et al, 2006;Takàč et al, 2011, Miernyk et al, 2011.…”