Plant Regeneration and Genetic Diversity of Regenerants from Seed-derived Callus of Reed (Phragmites communis Trinius)

Abstract

-This study was carried out to address an efficient in vitro regeneration system from seed-derived callus of Phragmites communis, and to evaluate genetic variations of the regenerants using ISSR markers. Shoot regeneration via calli was greatly influenced by N6 medium compared with MS medium, and plant regeneration frequency was 90% in N6 supplemented with BA 0.25 mg/L and BA 0.5 mg/L. According to ISSR analysis of the thirty regenerants, out of 94 loci detected overall, 16 were identified to be polymorphic with a rate (PR) of 17.0%. The mean gene diversity (h) of different in vitro condition was 0.03 and ranged from 0.008 for N6 with BA 5 mg/L, to 0.040 for MS with IAA 0.1 mg/L+kinetin 2 mg/L. The results indicate that the regenerants have a low genetic variation, and ISSR analysis is effective to detect genetic variation of regenerants.

Effect of the Phragmitis Rhizoma Aqueous Extract on the Pharmacokinetics of Docetaxel in Rats

“…Phragmitis rhizoma is the dried root stock of Phragmites communis Trinius, a perennial herbaceous plant that grows natively in the Northern Hemisphere [4]. It has been prescribed in traditional Korean and Chinese medicine to relieve fever, vomiting, dysuria, and constipation [4-6].…”

“…Phragmitis rhizoma is the dried root stock of Phragmites communis Trinius, a perennial herbaceous plant that grows natively in the Northern Hemisphere [4]. It has been prescribed in traditional Korean and Chinese medicine to relieve fever, vomiting, dysuria, and constipation [4-6]. Our previous study illustrated that the Phragmitis rhizoma aqueous extract (EPR) and its major component, p -hydroxycinnamic acid (HCA) significantly ameliorates DTX-induced myelotoxicity both in vitro and in vivo [7].…”

Anti-stress and Anti-histamine Effects of Phragmites communis, Salicornia herbacea, and Prunus mume

“…Histamine과 β-hexosaminidase는 비만 세포(mast cell)가 자극에 의해 활성화되었을 때 분비되는 것으 로 일반적으로 in vitro 염증, 알레르기 반응에 관한 탈과립 마커 로 사용되고 있다 (Park et al, 2008). 갈대(Phragmites communis T.)는 벼과(Gramineae)에 속하는 다년초로서 중국, 한국, 일본, 러시아, 쿠릴열도 등 북 반구의 온대지방에서 아한대에 넓게 분포한다 (Ryu et al, 2013). 뿌리는 한방에서 해열, 지갈, 이뇨제, 당뇨병의 소갈, 구 역질, 변비, 식중독 등 약용으로 사용되어 왔으며, 우리 주변에 서 흔하게 볼 수 있어 그 가치를 잊어버리고 있었다 (Mo et al, 2013;Lee et al, 2010 (Kim et al, 2014b).…”