Plant regeneration, developmental pattern and genetic fidelity of somatic embryogenesis derived Musa spp.

Abstract

Multiplication of banana cvs. Grand Naine (Musa AAA, Cavendish-sub group) and Rasthali (Musa AAB, Silk-sub group) were attempted through somatic embryogenesis. The influence of position of male flower buds, amino acid supplements in the induction of somatic embryogenesis and field performance of embryogenic cell suspension (ECS) derived banana plants were studied. Differentiated immature male flower buds positioned at 6–8 th bract whorl as explants showed better callus induction and somatic embryogenesis. Supplementation with glutamine at 400 mg L−1 along with 20:20 g L−1sucrose: maltose in maturation media induced a 10-fold increase in somatic embryo formation compared to control. Cotyledonary stage somatic embryos desiccated for 2 h showed higher germination compared to non-desiccated embryos. The plantlets generated were hardened, and the genetic fidelity of the plantlets was confirmed using ISSR marker. To check the field performance of ECS derived plants, plantlets were hardened and planted in the field along with meristem and sucker. During the field growth, these ECS derived plants were morphologically similar to those of control plants. In this experiment, it was observed that ECS derived banana plants displayed normal phenotype as that of plants grown from meristem and sucker. The protocol developed could be useful highly for large-scale micropropagation or genetic manipulation studies in these commercially important banana cultivars.

Effects of some gelling agents and their concentrations on conversion of oil palm polyembryoids into plantlets

“…It was reported earlier that a very high concentration of gelling agent resulted in high water stress which led to the difficulty of uptaking water and nutrient from culture medium [4]. Many authors reported the influence of gelling agents on the development of adventitious shoots, calli [13,14], somatic embryos [2,3,15], and protoplasts [16] under in vitro culture. These studies showed that the both type and quality of gelling agents also create problems related with hyperhydricity and necrosis of the tissue.…”

Changes in the Metabolite Profile during Micropropagation of Normal and Somaclonal Variants of Banana Musa AAA cv. Williams

“…However, the recognition of phenotypic characteristics and diagnosis of dwarfism can only be achieved by trained personnel at the end of the greenhouse acclimatization phase or in the field [8]. Molecular techniques such as random amplified polymorphic DNA (RAPD) [7,[16][17][18] amplified fragment length polymorphisms (AFLPs) [19] and inter simple sequence repeats (ISSRs) [17,[20][21][22][23] have been proposed to monitor SV in Musa. However, the relationship among the proposed molecular markers and the SVs was not described.…”

Establishment of a Standard Micropropagation Protocol for the Production of Uniform Planting Material in Musa Laterita (Section: Rhodochlamys).

“…Propagation through conventional methods using suckers is easy but takes longer duration for the largescale production of planting material and further they serve as the potential source of transmission of fungal pathogens, nematodes, weevils, and viruses etc. (Nandhakumar et al 2018). Therefore, the present study aimed at the rapid production of uniform and healthy planting material through development of a species-speci c protocol using shoot tip explants.…”

“…Male owers are orange yellow in colour, 2-3 cm long, 6-10 owers per bract and bi-seriate (Uma et al 2014). Most of the Rhodochlamys members are immune to Sigatoka leaf spot and Fusarium wilt diseases (Nandhakumar et al 2018).…”