Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro

Abstract

ABSTRACT

Micropropagation of an elite F1 watermelon (Citrullus lanatus) hybrid from the shoot tip of field grown plants

“…Maximum number of surviving explants (73%) and growing cultures (60%) were recorded at 5 mg l -1 BA and 0.1 mg l -1 IAA (Table 1). A combination of cytokinin and auxin in regenerating shoots has been described earlier (Dibax et al 2010). In some cases, a little amount of callus was found at the base of the explant.…”

Tissue Culture of Corymbia and Eucalyptus

“…camaldulensis, E. camaldulensis × E. tereticornis, and E. grandis × E. urophylla [88,103,107,120,149,150]. Half-strength MS medium with 1/10 KNO 3 and 2.5× MgSO 4 has been used for E. grandis shoots [104], and half-strength MS medium with full-strength vitamins, 2.66 µM riboflavin, and 0.93 µM β-carotene has been used recently for E. grandis × E. urophylla shoots [114].…”

“…Eucalypt shoots sometimes produce roots spontaneously in hormone-free medium or potting mix [45,46,64,88,92,96,104,113,119,143,144,150,161]. However, adventitious rooting on eucalypt shoots is usually induced with an auxin rooting hormone, typically IBA at a concentration between 0.49 and 49 µM (Table A1).…”

Histological analysis reveals the formation of shoots rather than embryos in regenerating cultures of Eucalyptus globulus

“…Hajari et al 2006;Dibax et al 2010). Both organogenesis and SE have been shown to be efficient techniques for the mass propagation of many species.…”

“…It was described earlier that buds regenerating from hypocotyls of E. globulus remained in a subepidermal position in the early stages; subsequently the shoots elongated and initiated new leaves while the epidermis became disrupted (Azmi et al 1997). Also during the regeneration of E. camaldulensis from cotyledonary explants, caulinar meristems developed in subepidermal positions (Dibax et al 2010). As a result of subsequent intensive cell divisions meristematic agglomerates broke the explant epidermis and emerged above the surface of the explant (Dibax et al 2010).…”