Plant regeneration from embryogenic callus and cell suspensions of Brachiaria brizantha

Cabral, G. B.; Carneiro, Vera Tavares de Campos; Rossi, Mônica Lanzoni; Silva, Joseane Padilha da; Martinelli, Adriana Pinheiro; Dusi, Diva Maria de Alencar

doi:10.1007/s11627-015-9690-0

Cited by 10 publications

(3 citation statements)

References 21 publications

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“…Festulolium has been developed by cross-breeding two closely related species of Festuca and Lolium, whereas backcrossing generated crops with more stable protein content in comparison to the parental line [42]. In another report, efficient biofortification has been achieved on temperate cultivars of Lolium and some tropical grasses including Pennisetum and Brachiaria [43]. Microbial biofortification has been achieved through the distinct sequence variants of fructan, as follows: Fructan 6G-fructosyltransferase increased the fructose levels in Lolium in hot climates.…”

Section: Fodder Cropsmentioning

confidence: 99%

Biofortification—A Frontier Novel Approach to Enrich Micronutrients in Field Crops to Encounter the Nutritional Security

et al. 2022

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Globally, many developing countries are facing silent epidemics of nutritional deficiencies in human beings and animals. The lack of diversity in diet, i.e., cereal-based crops deficient in mineral nutrients is an additional threat to nutritional quality. The present review accounts for the significance of biofortification as a process to enhance the productivity of crops and also an agricultural solution to address the issues of nutritional security. In this endeavor, different innovative and specific biofortification approaches have been discussed for nutrient enrichment of field crops including cereals, pulses, oilseeds and fodder crops. The agronomic approach increases the micronutrient density in crops with soil and foliar application of fertilizers including amendments. The biofortification through conventional breeding approach includes the selection of efficient genotypes, practicing crossing of plants with desirable nutritional traits without sacrificing agricultural and economic productivity. However, the transgenic/biotechnological approach involves the synthesis of transgenes for micronutrient re-translocation between tissues to enhance their bioavailability. Soil microorganisms enhance nutrient content in the rhizosphere through diverse mechanisms such as synthesis, mobilization, transformations and siderophore production which accumulate more minerals in plants. Different sources of micronutrients viz. mineral solutions, chelates and nanoparticles play a pivotal role in the process of biofortification as it regulates the absorption rates and mechanisms in plants. Apart from the quality parameters, biofortification also improved the crop yield to alleviate hidden hunger thus proving to be a sustainable and cost-effective approach. Thus, this review article conveys a message for researchers about the adequate potential of biofortification to increase crop productivity and nourish the crop with additional nutrient content to provide food security and nutritional quality to humans and livestock.

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Section: Fodder Cropsmentioning

confidence: 99%

Biofortification—A Frontier Novel Approach to Enrich Micronutrients in Field Crops to Encounter the Nutritional Security

et al. 2022

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“…Such improvements in traits could be aided by achievements in transformation and genetic marker techniques. Reproducible and high efficiency transformation has been developed for temperate cultivars of Festuca ( Wang and Ge, 2005 ; Zhang et al, 2006 ) and Lolium ( Bajaj et al, 2006 ; Badenhorst et al, 2016 ); and more recently for some of the tropical grasses such as Pennisetum ( Gondo et al, 2017 ) and Brachiaria ( Cabral et al, 2015 ). Some examples of gene editing forage crops to confer stress tolerances have been successful in aiding both biomass increases but also nutritional quality.…”

Section: Improving Forage Cropsmentioning

confidence: 99%

Improving the Yield and Nutritional Quality of Forage Crops

2018

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Despite being some of the most important crops globally, there has been limited research on forages when compared with cereals, fruits, and vegetables. This review summarizes the literature highlighting the significance of forage crops, the current improvements and some of future directions for improving yield and nutritional quality. We make the point that the knowledge obtained from model plant and grain crops can be applied to forage crops. The timely development of genomics and bioinformatics together with genome editing techniques offer great scope to improve forage crops. Given the social, environmental and economic importance of forage across the globe and especially in poorer countries, this opportunity has enormous potential to improve food security and political stability.

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“…In vitro plant regeneration protocols using as explants peeled seeds, isolated embryos from mature seeds, embryogenic calluses and embryogenic cell suspensions of B. brizantha cv. Marandu were developed (Cabral et al 2011(Cabral et al , 2015. Despite the progress in the development of tissue culture protocols, B. brizantha genetic transformation is still a challenge.…”

Section: )mentioning

confidence: 99%

Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics

Cabral

Carneiro

Gomes

et al. 2018

An. Acad. Bras. Ciênc.

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Brachiaria brizantha is a forage grass well adapted to tropical areas and cultivated in millions of hectares in Brazil. The apomictic mode of reproduction in this species, in addition to differences in ploidy between sexual and apomictic plants, impairs crossbreeding. The development of a methodology to transform apomictic cultivars will provide an option to introduce agronomic important traits to B. brizantha cv. Marandu. In addition, it will open the possibility to study in vivo the function of candidate genes involved in the apomictic reproduction. The objective of this work was to evaluate peeled seeds, isolated embryo from mature seeds, embryogenic calluses and embryogenic cell suspensions, as target explant for genetic transformation via biolistics. Plasmids bearing the marker genes gus and hptII under the control of the rice actin 1 promoter (pAct1-Os) or the maize ubiquitin 1 promoter (pUbi1Zm) were used. All the target-explants used were suitable for transient gene expression after bombardment, showing gus expression and resistance to hygromycin. Using embryogenic calluses and cell suspensions as target tissues, transgenic plants were regenerated and transgenes detected.

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Plant regeneration from embryogenic callus and cell suspensions of Brachiaria brizantha

Cited by 10 publications

References 21 publications

Biofortification—A Frontier Novel Approach to Enrich Micronutrients in Field Crops to Encounter the Nutritional Security

Biofortification—A Frontier Novel Approach to Enrich Micronutrients in Field Crops to Encounter the Nutritional Security

Improving the Yield and Nutritional Quality of Forage Crops

Genetic transformation of Brachiaria brizantha cv. Marandu by biolistics

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