Plant Regeneration from Leaf and Stem Segments of Shoots Raised in vitro from Mature Apple Trees

Welander, Margareta

doi:10.1016/s0176-1617(88)80238-4

Cited by 139 publications

(73 citation statements)

References 10 publications

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“…This agrees with the reports of Welander [19] and Liu [12]. Shoots grown in continuous darkness were etiolated.…”

Section: Discussionsupporting

confidence: 92%

“…As found also by Welander [19], the N6 medium was superior to LS for regeneration of adventitious shoots. The N6 medium differs from LS in several respects, having lower total salt content, lower N, Ca, and Mg content, a lower proportion of NH4-N, and higher concentrations of K and P. Changing the amount of N in LS or the NOa'NH 4 ratio in N6 did not increase regeneration of adventitious shoots and usually decreased it (Tables 4 and 5).…”

Section: Discussionsupporting

confidence: 72%

“…Use of whole leaves, even with the midrib cut as done here, is simpler than collecting leaf disks from in vitro grown leaves [3,4] or cutting the leaf blades into 1 mm wide strips [18,19].…”

Section: Discussionmentioning

confidence: 99%

“…The latter purpose would be of particular interest with apples where conventional breeding programs have had relatively little impact upon commercial apple production. Regeneration of adventitious shoots has been reported from apple cotyledons [7,8,9,12,13], embryo axes [6], seedling leaves and hypocotyls [11], callus initiated from both seedling leaves [11] and roots of intact microplants of M.25 rootstock [5], and from leaves or leaf disks obtained from in vitro propagated shoots [2,3,4,16,18,19] or greenhouse-grown plants [12] of mature clones. 1 The media used for these studies contained benzyladenine (BA) plus an auxin and, in some cases [6,7,8,9], coconut milk.…”

Section: Introductionmentioning

confidence: 99%

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Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars

Fasolo

Zimmerman

Fordham

1989

Plant Cell Tiss Organ Cult

175

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Abstract. Leaves taken from micropropagated shoots of several apple (Malus domesticaBorkh.) cultivars were cultured in vitro on Linsmaier & Skoog (LS) medium or the rice anther culture medium of Chu et al. (N6) containing various concentrations of either benzyladenine (BA) or thidiazuron (TDZ) plus naphthaleneacetic acid (NAA). Of the TDZ concentrations tested, 10/~M was most effective and it was equivalent to, or better than, 22~M BA for both the percentage of leaves regenerating shoots and number of shoots formed per regenerating leaf in almost every experiment. Lower concentrations of NAA (1.1 and 5.4/zM) gave best results with both BA and TDZ. N6 medium gave consistently better results than LS. Lowering total salt concentration or total N concentration of LS to that of N6 did not improve the response nor did changing the NO3:NH4 ratio. The 3-4 leaves on the most distal part of the shoot were most responsive and tended to form the most adventitious shoots. Placing the leaf cultures in the dark for the first 2-3 weeks of the culture period produced the best results. Optimum results were obtained by culturing leaves from the distal part of the shoot in the dark for 2 weeks on N6 medium containing 10#M TDZ and 1.1 or 5.4/zM NAA, then moving the cultures to 16 h daylight at a photon flux of 60pmol s ~m -~.

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“…This agrees with the reports of Welander [19] and Liu [12]. Shoots grown in continuous darkness were etiolated.…”

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars

Fasolo

Zimmerman

Fordham

1989

Plant Cell Tiss Organ Cult

175

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“…In adventitious shoot production, regeneration percentage and number of buds and shoots per explant were reduced when lingonberry leaves were cultured with their abaxial surface in contact with the medium (Debnath 2005c). Possibly, the adaxial surface is more responsive to nutrients because it is the last tissue of the leaf to cease growing and dividing (Welander 1988). The effect of polarity on regeneration was revealed by better callus growth and higher number of buds and shoots regenerated from the apical than from the central or basal segments of the lingonberry hypocotyls (Debnath 2003b).…”

Section: Adventitious Shoot Regenerationmentioning

confidence: 99%

Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry

Debnath¹

2007

Can. J. Plant Sci.

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Debnath, S. C. 2007. Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry. Can. J. Plant Sci. 87: 911-922. Vacinium fruits are genetically heterozygous species characterized as "not coming true from seed". Conventional methods for vegetative propagation of these species, although successful, are slow and labour-intensive, and few propagules can be produced from one plant of a selected clone or hybrid. Micropropagation techniques are important for clonal multiplication, germplasm improvement and gene conservation of Vaccinium fruits cultivated in Canada including blueberries, cranberries and lingonberries. In vitro propagation of these species using axillary bud proliferation and adventitious shoot regeneration has been investigated in a number of studies. Morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, and this dependence is genotype specific. The paper presents the progress in-depth of various aspects of the in vitro culture of Canadian Vaccinium species for their commercial production. Also discussed are techniques for clone rejuvenation and plant tissue culture for mass propagation of Canadian Vaccinium nuclear stocks. Les techniques de multiplication végétative in vitro revê-tent une grande importance pour la multiplication des clones, l'amélioration du matériel génétique et la conservation des gènes des fruits du genre Vaccinium cultivés au Canada, notamment le bleuet, la canneberge et l'airelle vigne-d'Ida. Diverses études se sont penchées sur la multiplication in vitro de ces espèces par prolifération des bourgeons et régénération des pousses adventives. La morphogénèse semble considérablement dépendre des régulateurs de croissance de la plante et du milieu de culture, une dépen-dance propre au génotype. L'article fait le point des progrès réalisés au niveau des divers aspects de la culture in vitro des espèces canadiennes du genre Vaccinium en vue de leur culture commerciale. On y présente aussi les techniques de rajeunissement des clones et de culture tissulaire pour la multiplication massive du matériel génétique des espèces canadiennes de Vaccinium.

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Apple

Malnoy

Korban

Boresjza-Wysocka

et al. 2008

Compendium of Transgenic Crop Plants

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The first report of transformed apple plants in 1989 raised expectations for new apple cultivars that would be better tasting, healthier, and easier to grow. Although, many different traits have now been introduced successfully into apple, no transformed cultivars have yet made it to commercial production. Most early reports on transformed apple described “proof of concept” experiments involving the development of regeneration protocols, and the choice of appropriate promoters and selectable markers. More recently, the focus has moved onto functional testing of traits of potential commercial interest. These traits can be grouped into two categories: horticultural production traits and fruit‐focused traits. Production traits of interest include bacterial, fungal and pest resistance, dwarfing, propagation, stress resistance, precocity, storage life, and self‐fertility. Examples of fruit‐focused traits include novel health properties, flavor, reduced browning, color, and reduced allergenicity. This review will consider reports of characters introduced into apple that are useful to growers and consumers, and looks toward future trends, targets, and challenges.

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Plant Regeneration from Leaf and Stem Segments of Shoots Raised in vitro from Mature Apple Trees

Cited by 139 publications

References 10 publications

Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars

Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars

Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry

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