Plant regeneration through somatic embryogenesis in hypocotyl explants of Trachyspermum ammi (L.) Sprague

Jasrai, Yogesh T.; Mehta, A. R.

doi:10.1007/bf00036147

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…It appears that cotyledon explants were more efficient than cotyledonary node explants, as the former showed more somatic embryos developed into heart/torpedo stage (Table 2). This is in line with the results of a previous study (Jasrai et al 1992), in which callus was induced from hypocotyl explants of T. ammi in the presence of 2,4-D (9 μM) and Kn (2.3 μM). Direct transfer of callus to a medium devoid of 2,4-D in the presence or absence of kinetin failed to induce embryogenesis.…”

Section: Resultssupporting

confidence: 92%

“…It has also been used for induction, maintenance, and plantlet regeneration from celery cell suspensions via somatic embryogenesis (Nadel et al 1989). However, in T. ammi, CH did not support the maturation of somatic embryos derived from hypocotyl explants and it promoted rhizogenesis (Jasrai et al 1992).…”

Section: Resultsmentioning

confidence: 98%

“…Plant regeneration from callus via somatic embryogenesis has been reported in T. ammi (Jasrai et al 1992;Sehgal and Abbas 1994). Somatic embryogenesis is an efficient method of micropropagation that offers a rapid means of producing a large number of genetically identical plants.…”

Section: Introductionmentioning

confidence: 92%

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Direct somatic embryogenesis from cotyledon and cotyledonary node explants in bishop’s weed Trachyspermum ammi (L.) sprague

Purohıt

Kothari

2007

In Vitro Cell.Dev.Biol.-Plant

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A three-stage procedure for embryogenesis in Trachyspermum ammi was developed from cotyledon and cotyledonary node explants cultured in Murashige and Skoog (MS) liquid medium supplemented with 0.2 mg l −1 2,4-dichlorophenoxyacetic acid (2,4-D). Globular somatic embryos without intervening callus phase developed in 4 wk. The development of embryos to heart and torpedo stages required second-stage subculture of the explants (along with developing embryos) in liquid medium with lower concentrations of 2,4-D. Further development of embryos required a third-stage subculture in hormone-free liquid medium supplemented with 100 mg l −1 casein hydrolysate. Regeneration of complete plantlets occurred after the fully developed somatic embryos were transferred to solidified half-strength MS medium supplemented with 1 mg l −1 gibberellic acid.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 98%