Plant stem cells and <i>de novo</i> organogenesis

Sang, Ya Lin; Cheng, Zhi Juan; Zhang, Xian Sheng

doi:10.1111/nph.15106

Cited by 76 publications

(59 citation statements)

References 38 publications

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“…Previous studies had shown that callus could be induced from aerial organs and resembles the tip of a root meristem (Sugimoto et al, 2019). The root meristem regulator genes, such as WOX5 and WOX11, had been verified to play important roles in callus initiation in A. thaliana and Oryza sativa (Hu et al, 2017;Cheng et al, 2018;Liu et al, 2018a;Sang et al, 2018). In this study, ZmWOX11 showed peak expression at D2 and dropped afterward.…”

Section: Discussionsupporting

confidence: 49%

Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation

Fang

Liu

et al. 2019

Front. Plant Sci.

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Maize transformation is highly based on the formation of embryonic callus, which is mainly derived from scutellum cells of the immature maize embryo. However, only a few genes involved in callus induction have been identified in maize. To reveal the potential genes involved in the callus induction of maize, we carried out a high-throughput RNA sequencing on embryos that were cultured for 0, 1, 2, 4, 6, and 8 days, respectively, on a medium containing or lacking 2,4-dichlorophenoxyacetic acid. In total, 7,525 genes were found to be induced by 2,4-dichlorophenoxyacetic acid and categorized into eight clusters, with clusters 2 and 3 showing an increasing trend related to signal transmission, signal transduction, iron ion binding, and heme binding. Among the induced genes, 659 transcription factors belong to 51 families. An AP2 transcription factors, ZmBBM2, was dramatically and rapidly induced by auxin and further characterization showed that overexpression of ZmBBM2 can promote callus induction and proliferation in three inbred maize lines. Therefore, our comprehensive analyses provide some insight into the early molecular regulations during callus induction and are useful for further identification of the regulators governing callus formation.

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Section: Discussionsupporting

confidence: 49%

Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation

Fang

Liu

et al. 2019

Front. Plant Sci.

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“…Whole plants can be regenerated from tissue samples such as branch cuttings or detached leaves via a callus [1], a mass of undifferentiated cells able to initiate shoot- and root-stem cells in the presence of the appropriate phytohormones [2]. Several genes have been shown to function in regenerating cells [3, 4]; however, the elucidation of the transcriptome profiles involved in the regeneration process of each cell is a major challenge as it is not currently possible to separate and identify the limited numbers of stem cells that randomly emerge in a callus during regeneration.…”

Section: Introductionmentioning

confidence: 99%

Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation

Kubo

Nishiyama

Tamada

et al. 2018

Preprint

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27 128 cDNA from the extracted contents of entire cells were rarely successful, presumably because the 129 central vacuole occupies ~ 90% of the plant cell volume [24] and accumulates RNases that 130 degrade RNA molecules [25]. Since the transcriptomes of the isolated nuclei are reported to be 131 similar to those of the whole cells [26, 27], we extracted cell contents including nuclei labelled 132 with a fusion protein (NGG) composed of a nuclear-localizing signal [28], sGFP (synthetic 133 green fluorescent protein) [29], and GUS (β-glucuronidase) [30] under an estrogen-inducible 134 system [18, 31]. 135 157 introduces several non-templated deoxycytosine residues to the 3′ ends of the first-strand cDNA 158 before synthesizing the complementary strands. Poly(dA) tailing extends the poly(dA) region at 159 the 3′ ends of the first-strand cDNAs using a terminal deoxynucleotidyl transferase, followed by 160 the annealing of a specific primer with 20-nt oligo (dT) sequences to the poly(dA) tail. After 161 second-strand synthesis using both types of cDNAs, we performed qPCR to detect cDNA 162

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“…It is a phenyl-urea compound that has been substantiated to have the cytokinin potency across plant species [24][25][26][27]. Growth regulators' signal conversions into metabolic activities and developmental morphologies in cells or organisms have been established in biology [28][29][30][31][32][33]. The concept is broadly utilized for downstream applications particularly in cell, tissue, and organ cultures as well as plant transformation across plant species [24,[34][35][36][37].…”

Section: Discussionmentioning

confidence: 99%

High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron

Matand¹,

Shoemake

2020

BMC Plant Biol

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Background: Daylilies are a lucrative crop used for its floral beauty, medicinal proprieties, landscaping, fire prevention, nutritional value, and research. Despite the importance, daylilies remain extremely challenging for multiplying in vitro. The response difficulty is exacerbated because a few good protocols for daylilies micropropagation are generally difficult to reproduce across genotypes. An efficient strategy, currently applied at Langston University, is to systematically explore individual tissues or organs for their potential to micropropagation. This article is a partial report of the investigation carried out under room environmental conditions and focuses on developing an efficient daylilies in vitro propagation protocol that uses the stem tissue as the principal explant. Results: In less than three months, using thidiazuron, the use of the stem tissue as the in vitro experimental explant was successful in inducing multiple shoots several folds greater than current daylilies shoot organogenesis protocols. The study showed that tissue culture can be conducted successfully under unrestricted room environmental conditions as well as under the controlled environment of a growth chamber. It also showed that splitting lengthwise stem explants formed multiple shoots several folds greater than cross-sectioned and inverted explants. Shoot conversion rate was mostly independent of the number of shoots formed per explants. The overall response was explant and genotype-dependent. Efficient responses were observed in all thidiazuron treatments. Conclusion: An efficient protocol, which can be applied for mass multiple shoots formation using the daylilies stem tissue as the main explant, was successfully developed. This could lead to a broad and rapid propagation of the crop under an array of environmental conditions to meet the market demand and hasten exogenous gene transfer and breeding selection processes.

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Plant stem cells and de novo organogenesis

Cited by 76 publications

References 38 publications

Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation

Transcriptome Profiling Predicts New Genes to Promote Maize Callus Formation and Transformation

Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation

High frequency in vitro regeneration of adventitious shoots in daylilies (Hemerocallis sp) stem tissue using thidiazuron

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