Plant Tissue Culture Techniques for Breeding

Bridgen, Mark P.; Houtven, Wouter Van; Eeckhaut, Tom

doi:10.1007/978-3-319-90698-0_6

Cited by 30 publications

(27 citation statements)

References 40 publications

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“…This methodology confers an absolute independence of climatic threats, plant pathogens and harsh agriculture management and large storage facilities of plant materials. Nevertheless, PTC should cope with their own difficulties, as it requires the investment for specialized equipment and consumables and the recruitment of trained staff to develop the associated methodologies (Bridgen et al, 2018;Patra et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

et al. 2020

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Plant nutrition is a crucial factor that is usually underestimated when designing plant in vitro culture protocols of unexploited plants. As a complex multifactorial process, the study of nutritional imbalances requires the use of time-consuming experimental designs and appropriate statistical and multiple regression analysis for the determination of critical parameters, whose results may be difficult to interpret when the number of variables is large. The use of machine learning (ML) supposes a cutting-edge approach to investigate multifactorial processes, with the aim of detecting non-linear relationships and critical factors affecting a determined response and their concealed interactions. Thus, in this work we applied artificial neural networks coupled to fuzzy logic, known as neurofuzzy logic, to determine the critical factors affecting the mineral nutrition of medicinal plants belonging to Bryophyllum subgenus cultured in vitro. The application of neurofuzzy logic algorithms facilitate the interpretation of the results, as the technology is able to generate useful and understandable “IF-THEN” rules, that provide information about the factor(s) involved in a certain response. In this sense, ammonium, sulfate, molybdenum, copper and sodium were the most important nutrients that explain the variation in the in vitro culture establishment of the medicinal plants in a species-dependent manner. Thus, our results indicate that Bryophyllum spp. display a fine-tuning regulation of mineral nutrition, that was reported for the first time under in vitro conditions. Overall, neurofuzzy model was able to predict and identify masked interactions among such factors, providing a source of knowledge (helpful information) from the experimental data (non-informative per se), in order to make the exploitation and valorization of medicinal plants with high phytochemical potential easier.

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Section: Introductionmentioning

confidence: 99%

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

et al. 2020

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“…Unlike spontaneous regeneration, induced two-step shoot regeneration from HR derived calli with either 2-ip or zeatin resulted in two unique shoots. Adventitious shoot regeneration with a callus intermediate phase could, however, result in somaclonal variation (Bridgen et al, 2018). T-DNA genes present in Reg1 and Reg2 were stably integrated since no residual bacteria were detected in the plant tissue.…”

Section: Discussionmentioning

confidence: 99%

Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

et al. 2020

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Rhizobium rhizogenes infects and transforms a wide range of plant species. It thereby introduces new genes located on transfer-DNA of the root inducing plasmid (pRi) into the plant genome and one of its abilities is to alter the host root system. Explants from pRi transformed roots from Sinningia speciosa were regenerated to create naturally transgenic Ri lines. The presence of rol and aux genes in the Ri lines was linked with altered growth characteristics: shorter peduncles, wrinkled leaves, delayed flowering and enhanced root growth. The potential of Ri lines for breeding was evaluated through consecutive backcrossing with the original host genotype. The progeny of reciprocal crosses showed non-Mendelian inheritance suggesting partial transmission of the of the aux and rol genes. The typical Ri phenotype observed in the primary Ri line was partially inherited. These results revealed that the Ri phenotype is a complex trait influenced by the genetic background of the Ri line.

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“…The tissue culture technique allows researchers to grow and manipulate plants under in-vitro sterile conditions and can be very useful to plant breeders (Bridgen et al, 2018). In fact, this technique can provide the diversities needed for selection the desired traits by inducing somaclonal variation.…”

Section: Introductionmentioning

confidence: 99%

Somatic embryogenesis of hypocotyl derived calli from an eggplant cultivar

Sabet

Maleki

Nasab

et al. 2020

AAS

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Optimization of tissue culture and regeneration conditions of eggplant is necessary for achieving different goals such as gene transformation and the development of somaclonal variations. In this study, hypocotyl explants ware used to produce callus in a medium containing different concentrations of NAA and BAP. Moreover, the concentration of the elements Ca, Mn, Mg, Fe and K were measured and analysed between embryogenic and non-embryogenic calli. For shoot elongation, embryogenic calli were transferred to a new culture medium containing 3.5, 4 and 4.5 mg l-1 BAP plus 2 mg l-1 GA3. Finally, produced shoots were rooted in a culture medium containing 1, 1.5 and 2 mg l-1 NAA. Results showed that the best treatment for the embryogenic callus induction was MS medium containing 0.5 mg l-1 BAP plus 0.25 mg l-1 NAA. Two elements, Fe and K, had the highest amount in non-embryogenic calli compare to the embryogenic one. For plant regeneration, MS medium containing 4.5 mg l-1 BAP plus 2 mg l-1 GA3 and 2 mg l-1 NAA were the best treatments for shooting and rooting, respectively. In this study, the best treatments for plant regeneration produced 35 shoots from an explant with 92 % shooting. This regeneration protocol could be useful for gene transformation and micro-propagation studies.

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Plant Tissue Culture Techniques for Breeding

Cited by 30 publications

References 40 publications

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

Machine Learning Unmasked Nutritional Imbalances on the Medicinal Plant Bryophyllum sp. Cultured in vitro

Segregation of rol Genes in Two Generations of Sinningia speciosa Engineered Through Wild Type Rhizobium rhizogenes

Somatic embryogenesis of hypocotyl derived calli from an eggplant cultivar

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