Plant virus DNA replication processes in Agrobacterium: insight into the origins of geminiviruses?

Rigden, Justin E.; Dry, Ian B.; Krake, L. R.; Rezaian, M. Ali

doi:10.1073/pnas.93.19.10280

Cited by 55 publications

(46 citation statements)

References 25 publications

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“…It has been suggested that geminiviruses have evolved from prokaryotic circular ssDNA replicons (9,12). If this scenario is correct, one would expect to observe geminiviruses in different eukaryotic kingdoms.…”

Section: Replication Initiator Motifs In Helitronsmentioning

confidence: 99%

“…They encode only one protein, similar to the replication initiator proteins (Rep) from known RC replicons (6)(7)(8)(9). There are three groups of episomal replicons using RCR: circular single-stranded DNA (ssDNA) bacteriophages (10), plasmids of bacteria or archaea (11), and geminiviruses (circular ssDNA viruses replicating in plant cells) (12). Usually replication of RC replicons is catalyzed by the nuclease͞ligase activity of Rep and is assisted by host DNA helicases and ssDNA-binding proteins (SSBs) (6)(7)(8)(9)(10)(11)(12).…”

mentioning

confidence: 99%

“…There are three groups of episomal replicons using RCR: circular single-stranded DNA (ssDNA) bacteriophages (10), plasmids of bacteria or archaea (11), and geminiviruses (circular ssDNA viruses replicating in plant cells) (12). Usually replication of RC replicons is catalyzed by the nuclease͞ligase activity of Rep and is assisted by host DNA helicases and ssDNA-binding proteins (SSBs) (6)(7)(8)(9)(10)(11)(12). Here, we report a previously unknown category of eukaryotic DNA transposons, named Helitron, that transpose as RC replicons in the Arabidopsis thaliana, Oriza sativa, and Caenorhabditis elegans genomes.…”

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confidence: 99%

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Rolling-circle transposons in eukaryotes

Kapitonov

Jurka²

2001

Proc. Natl. Acad. Sci. U.S.A.

458

541

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All eukaryotic DNA transposons reported so far belong to a single category of elements transposed by the so-called ''cut-and-paste'' mechanism. Here, we report a previously unknown category of eukaryotic DNA transposons, Helitron, which transpose by rollingcircle replication. Autonomous Helitrons encode a 5 -to-3 DNA helicase and nuclease͞ligase similar to those encoded by known rolling-circle replicons. Helitron-like transposons have conservative 5 -TC and CTRR-3 termini and do not have terminal inverted repeats. They contain 16-to 20-bp hairpins separated by 10 -12 nucleotides from the 3 -end and transpose precisely between the 5 -A and T-3 , with no modifications of the AT target sites. Together with their multiple diverged nonautonomous descendants, Helitrons constitute Ϸ2% of both the Arabidopsis thaliana and Caenorhabditis elegans genomes and also colonize the Oriza sativa genome. Sequence conservation suggests that Helitrons continue to be transposed.

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Section: Replication Initiator Motifs In Helitronsmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Rolling-circle transposons in eukaryotes

Kapitonov

Jurka²

2001

Proc. Natl. Acad. Sci. U.S.A.

458

541

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show abstract

“…Rather than by cut and paste, these DNA elements are proposed to move through replication and strand replacement. This mode of propagation, called rolling circle transposition, has been reported previously in some insertional sequence groups of transposable elements in prokaryotes (Tavakoli et al, 2000), bacterial plasmids (Khan, 2000), and the circular single-stranded DNA of plant geminiviruses (Rigden et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

The Maize Genome Contains a Helitron Insertion

et al. 2003

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The maize mutation sh2-7527 was isolated in a conventional maize breeding program in the 1970s. Although the mutant contains foreign sequences within the gene, the mutation is not attributable to an interchromosomal exchange or to a chromosomal inversion. Hence, the mutation was caused by an insertion. Sequences at the two Sh2 borders have not been scrambled or mutated, suggesting that the insertion is not caused by a catastrophic reshuffling of the maize genome. The insertion is large, at least 12 kb, and is highly repetitive in maize. As judged by hybridization, sorghum contains only one or a few copies of the element, whereas no hybridization was seen to the Arabidopsis genome. The insertion acts from a distance to alter the splicing of the sh2 pre-mRNA. Three distinct intron-bearing maize genes were found in the insertion. Of most significance, the insertion bears striking similarity to the recently described DNA helicase-bearing transposable elements termed Helitrons . Like Helitrons , the inserted sequence of sh2-7527 is large, lacks terminal repeats, does not duplicate host sequences, and was inserted between a host dinucleotide AT. Like Helitrons , the maize element contains 5 TC and 3 CTRR termini as well as two short palindromic sequences near the 3 terminus that potentially can form a 20-bp hairpin. Although the maize element lacks sequence information for a DNA helicase, it does contain four exons with similarity to a plant DEAD box RNA helicase. A second Helitron insertion was found in the maize genomic database. These data strongly suggest an active Helitron in the present-day maize genome.

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“…These results suggested that induction of SlNAC1 in response to TLCV infection is mediated by REn, and also demonstrated that SlNAC1 is not induced nonspecifically in response to A. tumefaciens infection or wounding associated with the infiltration procedure. In a subsequent experiment, a TLCV derivative containing a mutation in the C3 gene that prevents translation of the REn protein (Rigden et al, 1996) was tested for its effect on SlNAC1 expression. The level of SlNAC1 transcript in tomato plants agroinoculated with the REn mutant was comparable to healthy controls 25 dpi ( Figure 7D, top).…”

Section: Tlcv Infection Induces the Expression Of Slnac1mentioning

confidence: 99%

A NAC Domain Protein Interacts with Tomato leaf curl virus Replication Accessory Protein and Enhances Viral Replication

et al. 2004

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Geminivirus replication enhancer (REn) proteins dramatically increase the accumulation of viral DNA species by an unknown mechanism. In this study, we present evidence implicating SlNAC1, a new member of the NAC domain protein family from tomato (Solanum lycopersicum), in Tomato leaf curl virus (TLCV) REn function. We isolated SlNAC1 using yeast (Saccharomyces cerevisiae) two-hybrid technology and TLCV REn as bait, and confirmed the interaction between these proteins in vitro. TLCV induces SlNAC1 expression specifically in infected cells, and this upregulation requires REn. In a transient TLCV replication system, overexpression of SlNAC1 resulted in a substantial increase in viral DNA accumulation. SlNAC1 colocalized with REn to the nucleus and activated transcription of a reporter gene in yeast, suggesting that in healthy cells it functions as a transcription factor. Together, these results imply that SlNAC1 plays an important role in the process by which REn enhances TLCV replication.

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Plant virus DNA replication processes in Agrobacterium: insight into the origins of geminiviruses?

Cited by 55 publications

References 25 publications

Rolling-circle transposons in eukaryotes

Rolling-circle transposons in eukaryotes

The Maize Genome Contains a Helitron Insertion

A NAC Domain Protein Interacts with Tomato leaf curl virus Replication Accessory Protein and Enhances Viral Replication

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