Plantas taniníferas e o controle de nematóides gastrintestinais de pequenos ruminantes

Oliveira, Lorena Mayana Beserra de; Beviláqua, Cláudia Maria Leal; Morais, Selene Maia de; Camurça-Vasconcelos, A.L.F.; Macedo, Iara Térsia Freitas

doi:10.1590/s0103-84782011001100019

Cited by 12 publications

(8 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…When the host is infected with adults, the function of nematode reproductive changes, reducing the EPG and pasture contamination (MARTÍNEZ-ORTÍZ-DE-MONTELLAN et al, 2010;JOSHI et al, 2011). These effects cause significant prospects for the control of nematodes by consumption of tanninipherous plants (OLIVEIRA et al, 2011). Therefore, the pomegranate has gained renewed interest as potential anti-parasitic due to their main component, the tannins.…”

Section: Introductionmentioning

confidence: 99%

Pomegranate extracts on larval inhibition of Haemonchus spp and Cooperia spp obtained from cattle

Anjos

Silva²,

Holsback³

et al. 2016

Biosci. J.

View full text Add to dashboard Cite

Consumers increasingly demand natural food and absent of toxic waste and medicated. The aim of this study was to evaluate the aqueous extract of dried and in natura stem from pomegranate on the larval inhibition of Haemonchus spp. and Cooperia spp. obtained from cattle. Dried and in natura aqueous extract from pomegranate stem (AEPS), negative control (autoclaved distilled water) and positive control (levamisole hydrochloride 0.05 mg / mL) were evaluated. Four mL of each treatment were used, and the results were evaluated in triplicate by egg counts per gram of feces (EPG), larval culture and counting of the larvae. The mean of the larvae that developed per gram of feces was submitted to logarithimic transformation log. (X + 10). The EPG had an average value of 3.620 eggs, identified in larval culture from Haemonchus spp. and Cooperia spp. Dried AEPS presented, by the reduction of larvae, effectiveness of 92.29% for Haemonchus spp. and 96.97% for Cooperia spp. In natura, this extract showed 80% of efficacy against Cooperia larvae, being considered moderately effective; however, for Haemonchus spp. this extract was not effective. These results are specially important for organic and agroecological systems of production, that not allow the use of chemical parasiticides. In conclusion, the use of dried AEPS showed potential anthelmintic and may represent important natural therapeutic option.

show abstract

Section: Introductionmentioning

confidence: 99%

Pomegranate extracts on larval inhibition of Haemonchus spp and Cooperia spp obtained from cattle

Anjos

Silva²,

Holsback³

et al. 2016

Biosci. J.

View full text Add to dashboard Cite

show abstract

“…Diante os aspectos negativos causados por moléculas de ação anti-helmíntica, estudos estão sendo desenvolvidos como alternativa de controle parasitário (Oliveira et al 2011), nesta direção pesquisas em etnoveteriná-ria tem mostrado o uso de inúmeras plantas medicinais na prevenção e controle de doenças dos animais e com comprovada atividade anti-helmíntica (Barrabí-Puerta & Arece-García 2013). Outro aspecto importante no uso de plantas é que além de possuírem compostos bioativos com propriedades medicinais, apresentam baixo custo (Sousa et al 2013).…”

Section: Introductionunclassified

Estudo da atividade anti-helmíntica do extrato etanólico de Jatropha mollissima (Pohl) Baill. (Euphorbiaceae) sob Haemonchus contortus em ovinos no semiárido paraibano

et al. 2014

View full text Add to dashboard Cite

1051 tigada a concentração de extrato com efeito bioativo, pelo teste de evolução da toxicidade do extrato etanólico de J. mollissima sobre o microcrustáceo Artemia salina, obtendo uma CL50 de 660,80µg/ml, que foi testada em coproculturas contendo larvas infectantes de Haemonchus contortus e em animais para a verificação da redução do OPG. Para o teste in vivo o extrato foi dissolvido em água para se obter as concentrações 660,80µg/ml e 1321,6µg/ml, foram coletadas fezes semanalmente e sangue quinzenalmente. This study aimed to evaluate the anthelmintic effect of Jatropha mollissima through in vitro and in vivo experiments. Initially we investigated the concentration of extract with bioactive effect, through the toxicity evolution test of the ethanol extract of J. mollissima on the microcrustacean Artemia salina, obtaining CL50 concentration of 660.80µg/ml, which was tested in fecal cultures containing infective larvae of Haemonchus contortus and in animals for the verification of OPG reduction. For in vivo test, the extract was dissolved in water to obtain concentrations of 660.80µg/ml and 1321.6µg/ml. Feces were collected weekly and blood was collected every fifteen days. As a result of in vitro test, the ethanol extract of the stem of J. mollissima proved toxic on A. salina, with CL50 less than 1000µg/ ml and inhibited the eggs hatching and the development of larvae of H. contortus, presenting an efficiency of 70.77%. In vivo test revealed that the extract is also effective in sheep, with a significant reduction in the count of OPG after 28 days of experiment, 47 and 44% of reduction in the groups treated with the extract, 7.5% in the untreated group of animals and 40.6% with ivermectin. Even parasitized, the animals remained clinically healthy and without anemia. The ethanol extract of the stem of Jatropha mollissima may represent an alternative to the control of sheep worms, because it slows the parasitic resistance.

show abstract

“…Esses testes apresentam baixo custo e são executados rapidamente, sendo utilizados na triagem de uma grande quantidade de plantas e seus compostos bioativos. Além de ovos e larvas de primeiro estágio (L1), larvas de terceiro estágio (L3) e nematoides adultos têm sido utilizados [60,70]. Os principais resultados obtidos em testes in vitro com OE estão descritos na Tabela 1.…”

Section: Efeito Anti-helmíntico De óLeos Essenciaisunclassified

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

André

Ribeiro

Oliveira

et al. 2018

Acta Scientiae. Vet.

Self Cite

View full text Add to dashboard Cite

Background: Gastrointestinal nematodes are one of the major health and economic problem of sheep and goats in the world. The control of these nematodes is carried out conventionally with synthetic anthelminths, which favored the selection of gastrointestinal nematode (GIN) populations multiresistant to anthelmintics. The emergence of anthelmintic resistance has stimulated the search for new alternatives to control small ruminant GIN, standing out the use of plants and their bioactives compounds, such as essential oils (EO). The objective of this review was to present the main characteristics and anthelmintic activity of EO, their isolated compounds and drug delivery systems in the control of GIN.Review: Essential oils are a complex blend of bioactive compounds with volatile, lipophilic, usually odoriferous and liquid substances. EO are composed of terpenes, terpenoids, aromatic and aliphatic constituents. EO has various pharmacological activities of interest in preventive veterinary medicine such as antibacterials, antifungals, anticoccicids, insecticides and anthelmintics. In vitro and in vivo tests are used to validate the anthelmintic activity of EO on GIN. In vitro tests are low cost screening tests that allow the evaluation of the anthelmintic activity of a large amount of bioactive compounds on eggs, first (L1) and third stage larvae (L3), and adult nematodes. The antiparasitic effect of EO is related to its main compound or to the interaction of the compounds. These bioactive compounds penetrate the cuticle of the nematodes by transcuticular diffusion, altering the mechanisms of locomotion, besides causing cuticular lesions. Following in vitro evaluation, the acute and sub-chronic toxicity test should be performed to assess the toxicity of the bioactive compounds and to define the dose to be used in in vivo tests. In vivo tests are more reliable because the anthelmintic effectiveness of bioactive compounds is evaluated after the metabolization process. The metabolization process of the bioactive compounds can generate metabolites that exhibit or not anthelmintic effectiveness. The in vivo tests assessing the anthelmintic effectiveness of bioactive compounds in sheep and goats are the fecal egg count reduction test and the controlled test. OE promoted reduction of egg elimination in faeces which may be related to cuticular and reproductive alterations in GIN, and reduction of parasite burden in in vivo tests. Due to the promising results obtained with OE in the in vivo tests, interest has been aroused in using nanotechnology as an alternative to increase the bioavailability of OE and consequently, potentializing its anthelmintic effect, reducing the dose and toxicity of the biocompounds. In addition to nanotechnology, the isolation and chemical modification of compounds isolated from OE have been employed to obtain new molecules with anthelmintic action and understand the mechanism of action of EO on the small ruminant GIN.Conclusion: The use of EO and their compound bioactive in the control of resistant populations of GIN is a promising alternative. The adoption of strategies in which natural products can replace synthetic anthelmintics, such as in dry periods and use synthetic anthelmintics in the rainy season when the population in refugia in the pasture is high, thus reducing the dissemination of GIN resistant populations. As perspective, the evaluation of pharmacokinetics and pharmacodynamics of these natural products should be performed so that one defines treatment protocols that optimize the anthelmintic effect.

show abstract

Plantas taniníferas e o controle de nematóides gastrintestinais de pequenos ruminantes

Abstract: RESUMO Os nematóides gastrintestinais são responsáveis

Cited by 12 publications

References 42 publications

Pomegranate extracts on larval inhibition of Haemonchus spp and Cooperia spp obtained from cattle

Pomegranate extracts on larval inhibition of Haemonchus spp and Cooperia spp obtained from cattle

Estudo da atividade anti-helmíntica do extrato etanólico de Jatropha mollissima (Pohl) Baill. (Euphorbiaceae) sob Haemonchus contortus em ovinos no semiárido paraibano

Essential Oils and Their Bioactive Compounds in the Control of Gastrointestinal Nematodes of Small Ruminants

Contact Info

Product

Resources

About