Plants: Genetic Transformation

Abstract

The majority of cultivated plants, including all major cereals, can be transformed either by Agrobacterium ‐mediated transformation or by particle bombardment, which are the two widely used methods for genetic manipulation of plants. Virus‐based vectors can be efficiently used for high transient expression of foreign proteins in transfected plants, providing a low cost alternative to stable transformation. Beside these three major methods, further techniques such as protoplast‐based direct gene transfer, tissue electroporation, silicon carbide fiber‐mediated transformation, sonoporation, and microinjection have yielded transgenic plants, and still find applications.

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