Plants genotoxicity as pollution bioindicator in Jordan using comet assay

Abstract

This study aimed to assess genotoxicity in wild plants grown in Jordan as a pollution bioindicator. Comet assay was used to evaluate the level of DNA damage in plants collected from different areas in Jordan. Significant differences in plant DNA damage index and frequency were observed among sites of collection. Results show that plants collected from Aqaba back road and Ghour Assaal had significantly higher damage values. In contrast, plants collected from Wadi Rum, Al Naqab Heights, Swaimeh/Deadsea and Alshoneh Aljanobyeh showed low levels of DNA damage. A similar trend was observed for lipid peroxidation rates. Furthermore, heavy metal analysis showed that plants collected from Aqaba back road and Aqaba airport had the highest Al, Cr, Fe, Cu, Zn, Cd and Pb contents. A significant correlation was observed between DNA Damage Index, DNA Damage Frequency, lipid peroxidation rate, soil Cu, Cd and Pb biomarkers, indicating that heavy metals pollution is a major source for genotoxicity in these plant species. Finally, our results approved the feasibility of using plants and Comet assay system as a diagnostic tool for pollution in any environment adversely affected by different pollution sources.

Assessment of Xenoestrogens in Jordanian Water System: Activity and Identification

“…It is clear that Almujeb water has the least detected compounds and that may be explained by the location, where the dam and collected water are far from human pollution, such as heavy traffic and industrial areas [ 102 ]. Therefore, the estrogenic activity was below the detection limit.…”

Organismos bioindicadores de metais pesados: uma revisão

“…O fluxo intenso nas estradas também é responsável pela grande quantidade de emissões de poluentes de metais pesados, Galal et al (2015) constataram que Plantago major, presente nos acostamentos das estradas, possuía concentrações de alumínio (Al) e Fe em quantidade maior que 1000 mg/kg -1 , e por esse motivo é considerado um hiperacumulador para esses metais e, consequentemente, um bom biomarcador. Plantas silvestres cultivadas na Jordânia, foram reconhecidas como bioindicadoras para metais pesados por genotoxicidade, já que foi apontada uma considerável correlação entre o índice de danos ao DNA, taxa de peroxidação lipídica, frequência de danos ao DNA, biomarcadores de Cu, Cd e Pb do solo, demonstrando que a poluição por metais pesados é a causa dominante da genotoxicidade nessas espécies de vegetais (KHATEEB, 2018).…”

The wild plant Gnaphalium lavandulifolium as a sentinel for biomonitoring the effects of environmental heavy metals in the metropolitan area of México Valley

“…There are several major advantages of using plant systems as model, some of them related to their reproductive nature, easy culture, and growth habitats. So, they should be considered in mutagenicity and genotoxicity screening and monitoring (Al Khateeb, 2018).…”