Plasma and RBCs antioxidant status in occupational male pesticide sprayers

Prakasam, A.; Sethupathy, S; Lalitha, S.

doi:10.1016/s0009-8981(01)00487-9

Cited by 147 publications

(89 citation statements)

References 24 publications

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“…Previous studies have reported that exposure to different categories of pesticides induces oxidative stress. 11,24 Uric acid is known to be an antioxidant plasma component. Hence, these observations suggest that the increase of this component in workers may occur in response to an oxidative stress induced by pesticides, but this remains to be confirmed.…”

Section: Discussionmentioning

confidence: 99%

Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers

Araoud

Neffeti

Douki

et al. 2012

J Expo Sci Environ Epidemiol

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Biomonitoring of effects in agricultural workers is necessary to assess the individual risk of handling pesticides. In this study, biochemical and haematological parameters were measured to evaluate the effects of exposure to these compounds in agricultural workers. The study was carried out in 110 workers and 97 control subjects. Several haematological and biochemical parameters were analysed. Assessment of haematological parameters revealed that the mean cell volume and haematocrit levels were significantly lower in workers than in controls (P ¼ 0.002 and 0.013, respectively), while mean corpuscular haemoglobin concentrations were higher in workers (Po0.001). There was also a significant inhibition of butyrylcholinesterase activity in workers compared with that in controls (Po0.001). Assessment of biochemical parameters further showed significantly higher activities of transferases, lactate dehydrogenase (Po0.001), alkaline phosphatase (ALP) (P ¼ 0.006) and creatine kinase (CK) (Po0.015), as well as higher levels of proteins (Po0.001), creatinine (P ¼ 0.001) and urea (P ¼ 0.001) in workers compared with controls, along with significantly higher uric acid levels (P ¼ 0.012). Furthermore, the number of years exposed to pesticides predicted higher activities of alanine aminotransferase, CK, ALP, as well as uric acid levels. Overall, chronic exposure to pesticides appeared to affect several biochemical parameters. These biomarkers seem to be indicative of adverse effects of pesticides in agricultural workers, confirming their use for routine monitoring of effects.

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Section: Discussionmentioning

confidence: 99%

Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers

Araoud

Neffeti

Douki

et al. 2012

J Expo Sci Environ Epidemiol

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“…Bu yüzden bazı zirai ilaç uygulayıcılarının geçmişte, ülkemizde 2008 yılında yasaklanan endosulfan etkili maddesine sahip tarım ilaçlarını da kullandıkları yapılan anketlerde belirlenmiştir. Zirai ilaçların antioksidan kapasiteyi azaltıp, oksidan kapasiteyi arttırarak, oksidatif strese sebep olduğunu gösteren birçok çalışma bildirilmiştir [20,[23][24][25]. Reaktif oksijen bileşiklerinin herhangi bir nedenle aşırı oluşumu veya antioksidan savunma sistemi ve onarım sistemlerindeki yetersizlikler sonucu oksidatif stres gelişir [26].…”

Section: Hscanbay Söğüt /Organik Ve Organik Olmayan Elmalar Ile çunclassified

Organik ve Organik Olmayan Elmalar ile Bu Elmaları Üreten Kişilerdeki Pestisit Kalıntıları ve Toplam Antioksidan Kapasiteleri

Canbay

Öğüt²

2017

SAÜ Fen Bilimleri Enstitüsü Dergisi

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ÖZPestisit (diazinon, chlorpyriphos, methidation, deltamethrin, cypermethrin) uygulaması ile üretilen elmalar ile doğal hayvan gübreleri ve ticari organik gübre uygulanan elmalarda ve bu elmaların üretiminde çalışan kişilerin kan serumunda, pestisit kalıntıları ve toplam antioksidan kapasiteleri araştırılmış ve karşılaştırma yapılmıştır. Çalışmada toplam 60 tarım işçisi ve bu işçilerin ürettikleri 60 elma örnek grubu incelenmiştir. Yapılan kromatografik analizlerde zirai ilaç uygulanan 5 elma örneğinde chlorpyriphos kalıntısı belirlenmiştir. Bunun sebebi elmaları örneklerinin alındığı çiftliklerde, toplamadan önce en son uygulanan ilacın chlorpyriphos içermesidir. Organik olarak üretilen elmalarda ise pestisit kalıntısına rastlanmamıştır. 60 tarım işçisinin hiçbirinin kanında pestisit kalıntısı rastlanmamıştır. Spektrofotometrik analizler sonucunda ise toplam antioksidan seviyeleri (TAK) bakımından iki grup elma arasında anlamlı bir fark bulunamamıştır (p>0.05). Ancak elma üretiminde zirai ilaç kullanan üreticilerin kanlarında tespit edilen toplam antioksidan kapasiteleri, organik elma üreten kişilerin kanlarındaki toplam antioksidan kapasiteleri ile karşılaştırıldığında anlamlı seviyede azalma belirlenmiştir (p<0.05). Bu sonuçlar, zirai ilaç uygulamalarının, elmada toplam antioksidan kapasitede bir değişime neden olmadığını göstermiştir.Anahtar Kelimeler: Elma, pestisit, kan serumu, TAK, chlorpyriphos Pesticide residues and total antioxidant capacity of organic and non-organic apples and farmers ABSTRACTPesticide residues and total antioxidant capacity were investigated and compared in apples produced using pesticides and organically produced apples and in blood serums of people (they were working in the production of apples). In the research, were examined the 60 farm workers and the 60 apple samples produced by these workers. In the determination of pesticide residues was used gas chromatography (GC) device. In determining of TAC was used spectrophotometer. In chromatographic analysis, identified

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“…In further experiments, the effect of plasma factors on the release of NO from SNVP and SIN-1 was investigated. WP were incubated with levels of CP that approximate plasma concentrations (0.4 g l À1 ; Ravin, 1961;Prakasam et al, 2001) for 1-min prior to the addition of SNVP (100 mM). Similarly, WP was incubated with SOD (500 U ml À1 ), Asc (100 mM), human serum albumin (HSA, 4%) for 1-min prior to the addition of SIN-1 (100 mM; n ¼ 4 for all experiments).…”

Section: No Electrode Measurementsmentioning

confidence: 99%

“…In experiments designed to establish the effect of plasma factors on SNVP and SIN-1, WP was reconstituted with CP at a level that approximates its concentration in plasma (0.4 g l À1 ; Ravin, 1961;Prakasam et al, 2001). CP was observed to greatly enhance the release of NO from SNVP ( Figure 3a).…”

Section: Effect Of Plasma Factors On the Generation Of No By Snvp Andmentioning

confidence: 99%

A potential role for extracellular nitric oxide generation in cGMP‐independent inhibition of human platelet aggregation: biochemical and pharmacological considerations

Crane

Rossi

Megson

2005

British J Pharmacology

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1 Nitric oxide (NO) is a potent inhibitor of platelet activation, that inhibits the agonist-induced increase in cytosolic Ca 2 þ concentration through both cGMP-dependent and independent pathways. However, the NO-related (NO x ) species responsible for cGMP-independent signalling in platelets is unclear. We tested the hypothesis that extracellular NO, but not NO þ or peroxynitrite, generated in the extracellular compartment is responsible for cGMP-independent inhibition of platelet activation via inhibition of Ca 2 þ signalling. 2 Concentration-response curves for diethylamine diazeniumdiolate (DEA/NO; a spontaneous NO generator), S-nitroso-N-valerylpenicillamine (SNVP; an S-nitrosothiol) and 3-morpholinosydnonomine (SIN-1; a peroxynitrite generator) were generated in platelet-rich plasma (PRP) and washed platelets (WP) in the presence and absence of a supramaximal concentration of the soluble guanylate cyclase inhibitor, ODQ (20 mM). All three NO x donors displayed cGMP-independent inhibition of platelet aggregation in PRP, but only DEA/NO exhibited cGMP-independent inhibition of aggregation in WP. 3 Analysis of NO generation using an isolated NO-electrode revealed that cGMP-independent effects coincided with the generation of substantial levels of extracellular NO (440 nM) from the NO x donors. 4 Reconstitution of WP with plasma factors indicated that the copper-containing plasma protein, caeruloplasmin (CP), catalysed the release of NO from SNVP, while Cu/Zn superoxide dismutase (SOD) unmasked NO generated from SIN-1. The increased generation of extracellular NO correlated with a switch to cGMP-independent effects with both NO x donors. 5 Analysis of Fura-2 loaded WP revealed that only DEA/NO inhibited Ca 2 þ signalling in platelets via a cGMP-independent mechanism. However, preincubation of SNVP and SIN-1 with CP and SOD, respectively, induced cGMP-independent inhibition of intraplatelet Ca 2 þ trafficking by the NO x donors. 6 Taken together, our data suggest that extracellular NO (440 nM) is required for cGMPindependent inhibition of platelet activation. Plasma constituents may play an important pharmacological role in activating cGMP-independent signalling by S-nitrosothiols or peroxynitrite generators.

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Plasma and RBCs antioxidant status in occupational male pesticide sprayers

Cited by 147 publications

References 24 publications

Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers

Adverse effects of pesticides on biochemical and haematological parameters in Tunisian agricultural workers

Organik ve Organik Olmayan Elmalar ile Bu Elmaları Üreten Kişilerdeki Pestisit Kalıntıları ve Toplam Antioksidan Kapasiteleri

A potential role for extracellular nitric oxide generation in cGMP‐independent inhibition of human platelet aggregation: biochemical and pharmacological considerations

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