Plasma-based proteomics reveals lipid metabolic and immunoregulatory dysregulation in post-stroke depression

Zhan, Yuan; Yang, Yongtao; You, Hai-bo; Cao, Du; Liu, C.-Y.; Zhou, Chanjuan; Wang, Z.-Y.; Bai, Shunjie; Mu, Jun; Wu, Bo; Zhan, Qiongqiong; Xie, Peng

doi:10.1016/j.eurpsy.2014.03.004

Cited by 53 publications

(46 citation statements)

References 80 publications

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“…Human blood plasma is one of the most studied biological fluids and is the main sample type used for disease diagnosis [6–8]. In contrast to brain tissue and cerebrospinal fluid, human plasma can be sampled easily without invasive procedures.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion

et al. 2016

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The enormous depth complexity of the human plasma proteome poses a significant challenge for current mass spectrometry-based proteomic technologies in terms of detecting low-level proteins in plasma, which is essential for successful biomarker discovery efforts. Typically, a single-step analytical approach cannot reduce this intrinsic complexity. Current simplex immunodepletion techniques offer limited capacity for detecting low-abundance proteins, and integrated strategies are thus desirable. In this respect, we developed an improved strategy for analyzing the human plasma proteome by integrating polyethylene glycol (PEG) fractionation with immunoaffinity depletion. PEG fractionation of plasma proteins is simple, rapid, efficient, and compatible with a downstream immunodepletion step. Compared with immunodepletion alone, our integrated strategy substantially improved the proteome coverage afforded by PEG fractionation. Coupling this new protocol with liquid chromatography-tandem mass spectrometry, 135 proteins with reported normal concentrations below 100 ng/mL were confidently identified as common low-abundance proteins. A side-by-side comparison indicated that our integrated strategy was increased by average 43.0% in the identification rate of low-abundance proteins, relying on an average 65.8% increase of the corresponding unique peptides. Further investigation demonstrated that this combined strategy could effectively alleviate the signal-suppressive effects of the major high-abundance proteins by affinity depletion, especially with moderate-abundance proteins after incorporating PEG fractionation, thereby greatly enhancing the detection of low-abundance proteins. In sum, the newly developed strategy of incorporating PEG fractionation to immunodepletion methods can potentially aid in the discovery of plasma biomarkers of therapeutic and clinical interest.

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Section: Introductionmentioning

confidence: 99%

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion

et al. 2016

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“…Recent evidence has suggested an important role of lipids in the aetiology and treatment of depression [48,49]. Modern analytical platforms have also been applied in depression biomarker research [50][51][52][53].…”

Section: Affective Disordersmentioning

confidence: 99%

Application of fatty acid and lipid measurements in neuropsychiatry

Grela

Rachel

Cole

et al. 2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Abstract:The importance of lipids in the understanding of disease states is constantly increasing. Whilst the link between metabolic disorders and lipids seems to be clear, interpreting lipid regulation in the context of neuropsychiatric disorders is a new approach. Mental disorders account for almost 15% of the total global disease burden with Alzheimer's disease, depression or schizophrenia being amongst the most widespread mental disorders in the general population. For this reason rapid and early diagnosis is crucial and finding the right biomarkers is of great importance. Lipids appear to be essential in learning the aetiopathology of neuropsychiatric diseases as well as in biomarker research as they are most abundantly present in the brain. This study discusses recent findings in neuropsychiatry in the context of lipid analysis.

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“…In their validation experiments significant differences were found in Apo‐B100, A2M, VTDB, and Ceroplasmin . Zhan et al used iTRAQ to study plasma profiles of subjects with post stroke depression, stroke, and healthy controls ( n = 35 for each group) …”

Section: Applications Of Isobaric Labeling For Serum and Plasma Protementioning

confidence: 99%

“…182 Zhan et al used iTRAQ to study plasma profiles of subjects with post stroke depression, stroke, and healthy controls (n = 35 for each group). 127 In an investigation of markers of traumatic brain injury, Hergenroeder et al compared serum protein profiles from patients with severe brain injury (n = 11) to age-, sex-and race-matched volunteers. SAA, CRP and RBP4 levels were identified and found to be robust indicators of injury.…”

Section: Diseases Injuries and Afflictions Of The Brainmentioning

confidence: 99%

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

Moulder

Bhosale

Goodlett

et al. 2017

Mass Spectrometry Reviews

128

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Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.

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Plasma-based proteomics reveals lipid metabolic and immunoregulatory dysregulation in post-stroke depression

Cited by 53 publications

References 80 publications

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion

Enhanced Detection of Low-Abundance Human Plasma Proteins by Integrating Polyethylene Glycol Fractionation and Immunoaffinity Depletion

Application of fatty acid and lipid measurements in neuropsychiatry

Analysis of the plasma proteome using iTRAQ and TMT‐based Isobaric labeling

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