Plasma cytokine expression after lower-limb compression in rats

Sgarbi, Mauricio Wanderley Moral; Júnior, Bomfim Alves Silva; Peres, Cleto Kaveski; Loureiro, Tatiana Carolina Alba; Curi, Rui; Soriano, Francisco; Ribeiro, Daniel Araki; Velasco, Irineu Tadeu

doi:10.1016/j.rboe.2014.12.004

Cited by 2 publications

(2 citation statements)

References 18 publications

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“…Elastic bandages have also been used as crush material. Latex bandages were firmly attached to both hind limbs simultaneously in 10 tight turns, which provided 300 mm Hg pressure on rat's legs (27,28). The same research team then slightly adjusted the crush strategy, using two 5 cm wide Esmarch bandages to compress the right hind paw with the same pressure (29).…”

Section: Compression By Elasticated Materialsmentioning

confidence: 99%

Systemic Review of Animal Models Used in the Study of Crush Syndrome

Qing

Chen

et al. 2022

Shock

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Crush syndrome (CS), also known as traumatic rhabdomyolysis, is the leading cause of death following extrication from structural collapse due to earthquakes. Due to the unfeasibility of human studies, animal models are used to study crush syndrome pathophysiology, including biochemistry and treatment regimes. The aim of this systematic literature review was to identify the differences and benefits of various animal models used in the study of CS and provide valuable information for design of future research. A systematic search was conducted in two methods: with the filters ''(crush syndrome) AND (crush muscle injury)'' and with the keywords ''(crush syndrome) AND (animal model)'' covering all articles in the PubMed databases. The search generated 378 articles. After screening abstracts, 91 articles were retrieved and read, then 11 repeated articles were removed and 2 reference papers were included. We finally reviewed 82 original articles. There appear to be two primary methods employed for inducing crush syndrome in animal models, which are chemically induced injury and physically induced injury. Chemical method mainly includes intramuscular (IM) injection of tissue extract solution and IM injection of 50% glycerine. Physical method can be classified into invasive and non-invasive physical compression by elasticated material, inflatable band and heavy load. Various species of animals have been used to study CS, including mice (13.4%), rats (68.3%), rabbits (11.0%), canines (4.9%), goats (1.2%), and pigs (1.2%). Small animals are suitable for researches exploring the mechanism of disease or drug efficacy while large animals can work better with clinical application-related researches. In regard to the choice of modeling method, compressing the certain muscle of animals by heavy things is superior to others to cause systemic trauma-related rhabdomyolysis signs. In addition, due to the significant burden of crush injuries on animals, further attention shall be paid to the selection of the most suitable anesthetics and appropriate analgesics.

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Section: Compression By Elasticated Materialsmentioning

confidence: 99%

Systemic Review of Animal Models Used in the Study of Crush Syndrome

Qing

Chen

et al. 2022

Shock

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“…In addition to the known myoglobinuria, which evolves to renal failure, in experimental models, CS also appears to be associated with a systemic inflammatory response mediated by neutrophils and cytokines, the main shock organ being+ the lungs. 4 , 5 , 6 , 7 , 8 …”

Section: Introductionmentioning

confidence: 99%

Comparison of the effects of volemic reposition with 7.5% NaCl or blood in an experimental model of muscular compression and hemorrhagic shock

Sgarbi

Júnior

Pires

et al. 2018

Revista Brasileira de Ortopedia (English Edition)

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ObjectiveCrush syndrome is characterized by traumatic muscular injuries with severe systemic clinical repercussions. The systemic inflammatory reaction characterized acutely by infiltration of neutrophils in the lungs has been studied as part of the spectrum of crush syndrome. Experimental research may demonstrate alternative treatments for crush syndrome. The authors studied the hypothesis that hypertonic saline solution (7.5% NaCl) could minimize the local and systemic effects in a model of muscular compression and hemorrhagic shock.MethodsRabbits were submitted to a new model of muscle compression associated with hemorrhagic shock. Compression was applied through an Esmarch bandage, used for 1 h on the entire right lower limb. Hemorrhagic shock was induced for 1 h by dissection and catheterization of the carotid artery. Blood replacement or hypertonic saline solution was used to treat the shock. Biochemical analysis of plasma, quantification of muscular edema, and infiltration of inflammatory cells in the lungs were carried out.ResultsAnimals treated with hypertonic solution presented the same hemodynamic response as the blood treated patients, less water in the compressed muscles and less infiltration of inflammatory cells in the lungs. The blood group presented hypocalcemia, a facet of crush syndrome.ConclusionsThe proposed model was effective for the study of crush syndrome associated with hemorrhagic shock. The treatment with hypertonic solution showed benefits when compared with blood volume replacement.

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Plasma cytokine expression after lower-limb compression in rats

Cited by 2 publications

References 18 publications

Systemic Review of Animal Models Used in the Study of Crush Syndrome

Systemic Review of Animal Models Used in the Study of Crush Syndrome

Comparison of the effects of volemic reposition with 7.5% NaCl or blood in an experimental model of muscular compression and hemorrhagic shock

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