Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis

Schmelz, Martin; Luz, O.A; Averbeck, Beate; Bickel, Andreas

doi:10.1016/s0304-3940(97)00494-1

Cited by 166 publications

(125 citation statements)

References 20 publications

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“…Histamine is also a mediator that has traditionally been known to stimulate axon reXex, neurogenic Xare and itch in the skin. In addition, CGRP has been shown to be released in the Xare area elicited by histamine iontophoresis [166]. The eVect of histamine on cutaneous nerves and Xare can be controlled by topical anaesthetics [150] and by nedocromil sodium and loop diuretics, frusemide and bumetadine, drugs which appear to inhibit the Na + /K + /2Cl ¡ cotransporter in the sensory nerve membranes [206].…”

Section: Interactions Between Mast Cells and Sensory Nerves In Psoriasismentioning

confidence: 99%

Is there a role for mast cells in psoriasis?

et al. 2008

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Mast cells have traditionally been considered as eVector cells in allergy but during the last decade it has been realized that mast cells are essentially involved in the mechanisms of innate and acquired immunity. Upon activation by anaphylactic, piecemeal degranulation or degranulation-independent mechanisms mast cells can secrete rapidly or slowly a number of soluble mediators, such as serine proteinases, histamine, lipid-derived mediators, cytokines, chemokines and growth factors. Mast cells can express cell surface co-stimulatory receptors and ligands, and they can express MHC class II molecules and thereby present antigens. These soluble factors and cell surface molecules can interact with other cells, such as endothelial cells, keratinocytes, sensory nerves, neutrophils, T cell subsets and antigen presenting cells which are essential eVectors in the development of skin inXammation. Besides promoting inXammation, mast cells may attempt in some circumstances to suppress the inXammation and epidermal growth but the regulation between suppressive and proinXammatory mechanisms is unclear. Psoriasis is characterized by epidermal hyperplasia and chronic inXammation where tryptase-and chymase-positive MC TC mast cells are activated early in the developing lesion and later the cells increase in number in the upper dermis with concomitant expression of cytokines and TNF superfamily ligands as well as increased contacts with neuropeptide-containing sensory nerves. Due to the intimate involvement of mast cells in immunity and chronic inXammation the role of mast cells in psoriasis is discussed in this review.

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Section: Interactions Between Mast Cells and Sensory Nerves In Psoriasismentioning

confidence: 99%

Is there a role for mast cells in psoriasis?

et al. 2008

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“…They were used experimentally in both animal models and humans confi gured as either a linear fl owthrough membrane or a concentric dialysis probe, to recover serum proteins, neuropeptides, cytokines, and growth factors. [14][15][16] Since then, commercially available polyethersulfone microdialysis probes with an MWCO of 6000 to 100 000 Da have been used to recover a wide range of molecular species ranging in size from 3000 to 120 000 Da. [17][18][19][20] Some of these studies are summarized in Table 1 .…”

Section: Principles Of Dialysis Of Large Moleculesmentioning

confidence: 99%

Microdialysis of large molecules

Clough

2005

AAPS J

113

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“…In each lower leg, two semi-permeable microdialysis fibres (cutoff 3000 kDa) were inserted intradermally (16). Perfusion rate was 4 ll/min.…”

Section: Experimental Designmentioning

confidence: 99%

Inhibition of neuropeptide degradation suppresses sweating but increases the area of the axon reflex flare

Schlereth

Breimhorst

Werner

et al. 2013

Experimental Dermatology

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The neuropeptides CGRP (calcitonin gene-elated peptide) and substance P (SP) mediate neurogenic inflammation. Both are degraded by the neutral endopeptidase (NEP) which can be blocked by phosphoramidon. The aim was to evaluate the effect of NEP inhibition on sweating and vasodilatation. Dermal microdialysis was performed on the skin of 39 subjects. Two fibres were perfused with phosphoramidon (0.01%, 0.02% or 0.2%), two with saline. Acetylcholine (ACh) was either added to the microdialysis perfusate (n = 30, 10 À2 M) or thermoregulatory sweating was induced (n = 9). Co-application of phosphoramidon reduced cholinergic and thermoregulatory sweating. However, the flare size -a localized increase in superficial blood flow after ACh-application -was significantly increased. The increase in flare size is most probably due to increased CGRP levels. The inhibition of sweating by phosphoramidon may involve an increase in SP, a reduction in CGRP-degradation fragments or a direct inhibitory action of phosphoramidon.

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Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis

Cited by 166 publications

References 20 publications

Is there a role for mast cells in psoriasis?

Is there a role for mast cells in psoriasis?

Microdialysis of large molecules

Inhibition of neuropeptide degradation suppresses sweating but increases the area of the axon reflex flare

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