Plasma inactivation of microorganisms on sprout seeds in a dielectric barrier discharge

Butscher, Denis; Loon, Hanne Van Van; Waskow, Alexandra; Rohr, Philipp Rudolf von; Schuppler, Markus

doi:10.1016/j.ijfoodmicro.2016.09.006

Cited by 110 publications

(115 citation statements)

References 76 publications

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“…Edible sprouts, such as alfalfa (Mahon et al., ; Ponka et al., ; van Beneden et al., ), fenugreek (EFSA, ), and mung beans (de Jong, Oberg, & Svenungsson, ; O'Mahony et al., ; van Duynhoven et al., ) are also relevant fresh produce involved in microbial disease outbreaks. The sprouting of seeds is a critical step, as it is normally done in conditions that favor microbial growth (Buck, Walcott, & Beuchat, ; Butscher, Van Loon, Waskow, von Rohr, & Schuppler, ; Peñas, Gómez, Frías, & Vidal‐Valverde, ). Contaminated seeds represent the main contamination vector for sprouts (Harris et al., ; Ponka et al., ; Proctor, Hamacher, Tortorello, Archer, & Davis, ; van Beneden et al., ; van Duynhoven et al., ), as microorganisms present on the seeds may become internalized during the germination process (Liu, Cui, Walcott, & Chen, ).…”

Section: Resultsmentioning

confidence: 99%

Microbial Contamination of Fresh Produce: What, Where, and How?

Machado-Moreira

Richards

Brennan

et al. 2019

Comp Rev Food Sci Food Safe

185

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Promotion of healthier lifestyles has led to an increase in consumption of fresh produce. Such foodstuffs may expose consumers to increased risk of foodborne disease, as often they are not subjected to processing steps to ensure effective removal or inactivation of pathogenic microorganisms before consumption. Consequently, reports of ready‐to‐eat fruit and vegetable related disease outbreak occurrences have increased substantially in recent years, and information regarding these events is often not readily available. Identifying the nature and source of microbial contamination of these foodstuffs is critical for developing appropriate mitigation measures to be implemented by food producers. This review aimed to identify the foodstuffs most susceptible to microbial contamination and the microorganisms responsible for disease outbreaks from information available in peer‐reviewed scientific publications. A total of 571 outbreaks were identified from 1980 to 2016, accounting for 72,855 infections and 173 deaths. Contaminated leafy green vegetables were responsible for 51.7% of reported outbreaks. Contaminated soft fruits caused 27.8% of infections. Pathogenic strains of Escherichia coli and Salmonella, norovirus, and hepatitis A accounted for the majority of cases. Large outbreaks resulted in particular biases such as the observation that contaminated sprouted plants caused 31.8% of deaths. Where known, contamination mainly occurred via contaminated seeds, water, and contaminated food handlers. There is a critical need for standardized datasets regarding all aspects of disease outbreaks, including how foodstuffs are contaminated with pathogenic microorganisms. Providing food business operators with this knowledge will allow them to implement better strategies to improve safety and quality of fresh produce.

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Section: Resultsmentioning

confidence: 99%

Microbial Contamination of Fresh Produce: What, Where, and How?

Machado-Moreira

Richards

Brennan

et al. 2019

Comp Rev Food Sci Food Safe

185

View full text Add to dashboard Cite

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“…Initially building on advances in plasma-assisted material fabrication 6–9, 10 , exhaust gas purification 11 , and wastewater treatment 12, 13 , significant progress has been made in the use of plasmas to selectively induce apoptosis in cancer cells 14–16 , to eliminate bacterial biofilms on living surfaces and promote wound healing 17, 18 , for pathogenic microorganism inactivation and removal from solid and liquid media 19, 20 , for mutation breeding 21 and for agricultural production 4, 22, 23 . The unique advantage of using plasmas over other methods of biological stimulation stems from its multi-modal activity, specifically the simultaneous production of chemical species, including reactive oxygen species (ROS) and reactive nitrogen species (RNS) noted for their catalytic activity and biological significance, highly-energetic electrons, electromagnetic radiation and thermal effects, which individually and synergistically affect the treated target 24, 25 .…”

Section: Introductionmentioning

confidence: 99%

“…In agriculture, these effects have been exploited to induce stress and thus selectively stimulate seed germination 22, 26 , decontaminate seed surfaces 3 , improve seed disease resistance 27 , and enhance biochemical processes associated with higher crop yields 5 . Of particular value is the ability of plasma treatment to effectively decontaminate the surface of the seed at the same dose as that required to stimulate germination and growth 28 , and without compromising the quality and safety of these seeds as a food source 20 .…”

Section: Introductionmentioning

confidence: 99%

Spectral characteristics of cotton seeds treated by a dielectric barrier discharge plasma

Wang

Zhou

Groot

et al. 2017

Sci Rep

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Cold atmospheric plasma has recently emerged as a simple, low-cost and efficient physical method for inducing significant biological responses in seeds and plants without the use of traditional, potentially environmentally-hazardous chemicals, fungicides or hormones. While the beneficial effects of plasma treatment on seed germination, disease resistance and agricultural output have been reported, the mechanisms that underpin the observed biological responses are yet to be fully described. This study employs Fourier Transform Infrared (FTIR) spectroscopy and emission spectroscopy to capture chemical interactions between plasmas and seed surfaces with the aim to provide a more comprehensive account of plasma−seed interactions. FTIR spectroscopy of the seed surface confirms plasma-induced chemical etching of the surface. The etching facilitates permeation of water into the seed, which is confirmed by water uptake measurements. FTIR of exhaust and emission spectra of discharges show oxygen-containing species known for their ability to stimulate biochemical processes and deactivate pathogenic microorganisms. In addition, water gas, CO2, CO and molecules containing −C(CH3)3− moieties observed in FTIR spectra of the exhaust gas during plasma treatment may be partly responsible for the plasma chemical etching of seed surface through oxidizing the organic components of the seed coat.

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“…To the best of our knowledge, no dc‐APGD‐based plasma‐reaction system working in a flow‐through mode has been proposed until now for eradication of bacterial phytopathogens. Former applications of APPs sources mainly involved eradication of human pathogens or microbial contaminants of food present on surfaces of Petri and quartz plates, seeds, plastic bottles, animal skin, or model materials imitating, for example, nails or hoofs (Butscher et al, ; Ermolaeva et al, ; Gabriel et al, ; Lu et al, ; Masaoka, ; Xiong et al, ). According to our best knowledge, Lu et al () were the first to use an APP source, in this case DBD, to inactivate with nearly 100% efficacy a plant pathogenic fungus Cladosporium fulvum .…”

Section: Resultsmentioning

confidence: 99%

“…Such measures could involve atmospheric pressure plasma (APP) sources of antimicrobial properties due to generated electrons, ions, heat, UV light, electric field, and various active species, and radicals (Ikawa, Kitano, & Hamaguchi, ). APP can be produced with the aid of various sources, including dielectric barrier discharges (DBDs) (Butscher, Van Loon, Waskow, Rudolf von Rohr, & Schuppler, ; Lu, Liu, Song, Zhou, & Niu, ; Miao & Yun, ; Shen et al, ; Tiede, Hirschberg, Viöl, & Emmert, ; Xiong, Roe, Grammer, & Graves, ; Yong et al, ), corona discharges (Kuwahara, Kuroki, Yoshida, Saeki, & Okubo, ; Masaoka, ), radio frequency discharges and plasmas (Akitsu, Ohkawa, Tsuji, Kimura, & Kogoma, ; Laroussi et al, ; Li et al, ; Matan, Puangjinda, Phothisuwan, & Nisoa, ; Ohkawa et al, ), microwave discharges (Gabriel et al, ; Park et al, ), and glow discharges (GD) (Dzimitrowicz et al, ,; Ikawa et al, ). To the best of our knowledge, DBD (Lu et al, ; Tiede et al, ; Xiong et al, ) and radio frequency discharges and plasmas (Laroussi et al, ; Ohkawa et al, ) have been implemented for microbiological and biomedical applications so far.…”

Section: Introductionmentioning

confidence: 99%

Rapid eradication of bacterial phytopathogens by atmospheric pressure glow discharge generated in contact with a flowing liquid cathode

Motyka

Dzimitrowicz

Jamróz

et al. 2018

Biotech & Bioengineering

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Diseases caused by phytopathogenic bacteria are responsible for significant economic losses, and these bacteria spread through diverse pathways including waterways and industrial wastes. It is therefore of high interest to develop potent methods for their eradication. Here, antibacterial properties of direct current atmospheric pressure glow discharge (dc-APGD) generated in contact with flowing bacterial suspensions were examined against five species of phytopathogens. Complete eradication of Clavibacter michiganensis subsp. sepedonicus, Dickeya solani, and Xanthomonas campestris pv. campestris from suspensions of OD ≈ 0.1 was observed, while there was at least 3.43 logarithmic reduction in population densities of Pectobacterium atrosepticum and Pectobacterium carotovorum subsp. carotovorum. Analysis of plasma-chemical parameters of the dc-APGD system revealed its high rotational temperatures of 2,300 ± 100 K and 4,200 ± 200 K, as measured from N and OH molecular bands, respectively, electron temperature of 6,050 ± 400 K, vibrational temperature of 4000 ± 300 K, and high electron number density of 1.1 × 10 cm . In addition, plasma treatment led to formation of numerous reactive species and states in the treated liquid, including reactive nitrogen and oxygen species such as NO , NH, H O , O , O, and OH. Further examination revealed that bactericidal activity of dc-APGD was primarily due to presence of these reactive species as well as to UVA, UVB, and UVC irradiation generated by the dc-APGD source. Plasma treatment also resulted in an increase in temperature (from 24.2 to 40.2 °C) and pH (from 6.0 to 10.8) of bacterial suspensions, although these changes had minor effects on cell viability. All results suggest that the newly developed dc-APGD-based system can be successfully implemented as a simple, rapid, efficient, and cost-effective disinfection method for liquids originating from different industrial and agricultural settings.

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Plasma inactivation of microorganisms on sprout seeds in a dielectric barrier discharge

Cited by 110 publications

References 76 publications

Microbial Contamination of Fresh Produce: What, Where, and How?

Microbial Contamination of Fresh Produce: What, Where, and How?

Spectral characteristics of cotton seeds treated by a dielectric barrier discharge plasma

Rapid eradication of bacterial phytopathogens by atmospheric pressure glow discharge generated in contact with a flowing liquid cathode

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