Plasma insulin patterns in the unanesthetized rat during intracardial infusion and spontaneous ingestion of graded loads of glucose

Insulin secretion by rat islet isografts of a defined endocrine volume after transplantation to three different sites

“…A second catheter was implanted into the left jugular vein for the infusion of glucose. This technique allows frequent blood sampling and continuous infusion of fluids in unanaesthetized and freely moving rats [9][10][11]. Blood loss due to the sampling procedure was replaced by transfusing blood from a normal donor rat at regular intervals.…”

“…These sites have previously proven to be highly effective in the rodents. Graft function was tested in unanaesthetized and unstressed freely moving rats [9][10][11] not only by an intravenous glucose tolerance test (IVGTT) but also by the response to a meal, which is the most physiological stimulus for insulin secretion.…”

Improved biocompatibility but limited graft survival after purification of alginate for microencapsulation of pancreatic islets

“…One or two weeks after the reoccurrence of hyperglycaemia, microcapsules were retrieved from the diabetic recipients by peritoneal lavage and processed for histological examination. At 4 to 6 weeks after implantation of successful encapsulated islet allografts the rats were provided with two cardiac catheters implanted via the right and left jugular vein for blood sampling and infusion of glucose, respectively, in unanaesthetized and freely moving rats [14,15]. Subsequently, the function of encapsulated islet allografts was tested by an intravenous glucose tolerance test (IVGTT) and by a meal test [6,13].…”

“…Subsequently, the total volume was calculated, assuming the islets to be perfect spheres. In each experiment we used four to five donors from which we isolated a total of 3500-4200 islets which corresponds to [10][11][12][13][14][15] Next, the pH of the solution was lowered to 3.5 under constant monitoring by addition of 2 N HCl plus 20 mmol/l NaCl. The solution was kept on ice to prevent hydrolysis of alginate.…”

Régulation métabolique de l'ingestion chez les monogastriques

“…Since glucose is a precursor of triglycerides deposited in adipose tissue and since oxidation of fatty acids in peripheral tissues has a glucose-sparing effect circulating glucose seems a likely candidate for the signal mediating the effect of fat deposits on food intake. (Steffens 1969(Steffens , 1970 Strubbe et Bouman, 1978 (Lagaillarde, 1985 (Lagaillarde, 1985). (Puis et al, 1977;Glick et Modan, 1977;Thomas et Mayer, 1978;Geiselman et Novin, 1982 (Liebelt et al, 1965;Schemmel et al, 1971;Chlouverakis et Hojuicki, 1974;Dark et al, 1984Dark et al, , 1985.…”