Plasma levels and urinary excretion of disodium cromoglycate after inhalation by human volunteers

Moss, Gerald

doi:10.1016/0041-008x(71)90041-x

Cited by 74 publications

(21 citation statements)

References 4 publications

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“…The strong acid character makes extraction of the drug from biological matrices difficult. Moss et al (4) reported a colorimetric method for cromoglycate in human urine. The methodology involved preliminary extraction of the diacid using Amberlite" resin followed by alkali decomposition and derivatization with diazotized p-nitroanaline and UV detection.…”

Section: Introductionmentioning

confidence: 99%

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Sodium Cromoglycate in Race Horses: Development of Enzyme-Linked Immunosorbent Assay (ELISA) Screening and Gas Chromatography/Mass Spectrometry Confirmation Techniques

Leavitt¹,

Farmer²,

Paterson³

et al. 1993

Canadian Society of Forensic Science Journal

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Development of a rapid and sensitive ELlSA for detection of sodium cromoglycate in equine urine is described. Detection limit of the assays was less than 1 ng/mL. Enzyme conjugate binding to immobilized sheep antibody was inhibited 50% by less than 10 ng/mL. Intraassay and interassay variabilities, as measured by percent coefficient of variation, were less than 9% and 15% respectively. The test was capable of reliably detecting cromoglycate in post administration urine samples for 6 hours following 80 mg nebulized or for 17 hours following an 80 mg intratracheal dose. Background and matrix effects were found to be minimal. The test was very specific -no compound has yet been found to significantly cross react.Investigations into extraction and analysis of cromoglycate using GC/MS are also reported. RESUMELe developpement d'une msthode "ELlSA" rapide et sensible pour le depistage du cromoglycate sodique dans I'urine de cheval est presents. La Iimite de detection de I'essai etait de moins de 1 ng/mL. Une inhibition de 50% de I'agglomeration du conjugat enzymatique aux anticorps immobilises de mouton n'a necesslte que moins de 10 ng/mL. Les varlabllltes intra-essai et inter-essai, tel que mesurees aI'aide des coefficients de variation, etaient de moins de 9 % et de 15 % respectivement. L'essai etait capable de deceler du cromoglycate de fac;on efficace dans des echantillons d'urine de cheval pour une periods de 6 heures suivant I'administration de 80 mg avec un aerosol doseur ou pour une perlode de 17 heures si les 80 mg de la drogue etait adrnlnlstres par voie lntratracheale. Les interferences provenant du type d'echantillons etaient minimales. L'essai s'est avere tres speclflque: aucun autre compose n'a ete trouve cl date qui pouvait reagir d'une tacon significative avec I'anticorps.Des enqstes portant sur I'extraction et I'analyse du cromoglycate aI'aide de "GC/MS" sont egalement presentees.

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Section: Introductionmentioning

confidence: 99%

“…It is effective in treatment of respiratory allergic diseases such as asthma. The drug has no antihistaminic, anti-inflammatory, decongestant or bronchodilator activity (4). It expresses its pharmacological action by inhibiting a step in the chain of biochemical events following union of antigen with reaginic antibody (5).…”

Section: Introductionmentioning

confidence: 99%

Sodium Cromoglycate in Race Horses: Development of Enzyme-Linked Immunosorbent Assay (ELISA) Screening and Gas Chromatography/Mass Spectrometry Confirmation Techniques

Leavitt¹,

Farmer²,

Paterson³

et al. 1993

Canadian Society of Forensic Science Journal

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“…The volume of each sample was measured, and an aliquot was refrigerated for later assay of DSCG content. Assays were carried out by methods described pre-viously (Moss, Jones, Ritchie & Cox, 1971; (Moss & Ritchie, 1970;Moss et al, 1971;Walker, Evans, Richards & Paterson, 1972). Hence the DSCG reaching the airways partially appears as unchanged drug in urine, and urinary excretion is a useful indicator of the extent to which successful deposition of DSCG in its target organ occurs.…”

Section: Methodsmentioning

confidence: 99%

Disposition of disodium cromoglycate administered in three particle sizes.

Curry

Taylor

Evans

et al. 1975

Brit J Clinical Pharma

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I Disodium cromoglycate (DSCG) was administered in three particle sizes to five human subjects. 2 Urinary excretion of DSCG, as a proportion of the dose, was highest following small particles; the lower values recorded following intermediate-sized and large particles were similar. 3 DSCG deposited in the mouth was highest following large particles; the lower values recorded following intermediate-sized and small particles were similar. 4 The data were examined in relation to the recent observation that the protective effect of small particles of DSCG is dramatically superior to that of large particles.

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“…Das Pulver wird nach Inhalation in der Lunge schnell resorbiert und erreicht bereits nach 20 Minuten die h6chsten Plasmakonzentrationswerte. Die Plasmahalbwertzeit betr~igt 80 Minuten [42,62], Es kann daher nicht ausgescb_lossen werden, daB m6g-ticherweise die langf¡ t~gliche Anwendung von DNCG ira ersten Krankheitsfall die schweren Komplikationen mitverursacht hat.…”

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