Plasma Lipidomics Profiling Reveals Biomarkers for Papillary Thyroid Cancer Diagnosis

Jiang, Nan; Zhang, Zhenya; Chen, Xianyang; Guo-fen, Zhang; Wang, Ying; Pan, Longfa; Yan, Chengping; Yang, Guangdie; Zhao, Li; Han, Jiarui; Xue, Teng

doi:10.3389/fcell.2021.682269

Cited by 27 publications

(25 citation statements)

References 35 publications

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“…The change of these lipids is believed due to the cancerization of thyroid tissues, and therefore, characteristic spectra of lipid metabolites could be used for diagnosis of disease. It is reported that lipid profile is a type of biomarkers in thyroid cancer ( Li D. et al, 2019 ; Jiang et al, 2021 ). The study of lipid metabolism of thyroid cancer represents a new field of research that has recently received increasing interest in cancer research.…”

Section: Resultsmentioning

confidence: 99%

Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization

Liu

Shen²,

Di³

et al. 2023

Front. Chem.

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Direct mass spectrometry (MS) analysis of human tissue at the molecular level could gain insight into biomarker discovery and disease diagnosis. Detecting metabolite profiles of tissue sample play an important role in understanding the pathological properties of disease development. Because the complex matrices in tissue samples, complicated and time-consuming sample preparation processes are usually required by conventional biological and clinical MS methods. Direct MS with ambient ionization technique is a new analytical strategy for direct sample analysis with little sample preparation, and has been proven to be a simple, rapid, and effective analytical tools for direct analysis of biological tissues. In this work, we applied a simple, low-cost, disposable wooden tip (WT) for loading tiny thyroid tissue, and then loading organic solvents to extract biomarkers under electrospray ionization (ESI) condition. Under such WT-ESI, the extract of thyroid was directly sprayed out from wooden tip to MS inlet. In this work, thyroid tissue from normal and cancer parts were analyzed by the established WT-ESI-MS, showing lipids were mainly detectable compounds in thyroid tissue. The MS data of lipids obtained from thyroid tissues were further analyzed with MS/MS experiment and multivariate variable analysis, and the biomarkers of thyroid cancer were also investigated.

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Section: Resultsmentioning

confidence: 99%

Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization

Liu

Shen²,

Di³

et al. 2023

Front. Chem.

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“…In particular, PCs and PEs were decreased in the patients with high-grade CIN and cervical cancer, whereas DAGs and free FAs were significantly increased. The analysis of plasma samples of papillary thyroid carcinomas (47 patients and 33 healthy controls) provided evidence that a few sphingolipids and methyl-PE 18:1/18:1 were up-regulated in cancer patients and could be used as potential diagnostic markers [ 70 ]. A recent study investigating plasma lipids for the early detection of malignant gliomas used large discovery ( n = 107), training ( n = 750), test ( n = 225) and validation cohorts ( n = 920) [ 71 ].…”

Section: The Lipidome Of Biofluids As a Source Of Potential Cancer Bi...mentioning

confidence: 99%

Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis

et al. 2023

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Altered cellular metabolism is a well-established hallmark of cancer. Although most studies have focused on the metabolism of glucose and glutamine, the upregulation of lipid metabolism is also frequent in cells undergoing oncogenic transformation. In fact, cancer cells need to meet the enhanced demand of plasma membrane synthesis and energy production to support their proliferation. Moreover, lipids are precursors of signaling molecules, termed lipid mediators, which play a role in shaping the tumor microenvironment. Recent methodological advances in lipid analysis have prompted studies aimed at investigating the whole lipid content of a sample (lipidome) to unravel the complexity of lipid changes in cancer patient biofluids. This review focuses on the application of mass spectrometry-based lipidomics for the discovery of cancer biomarkers. Here, we have summarized the main lipid alteration in cancer patients’ biofluids and uncovered their potential use for the early detection of the disease and treatment selection. We also discuss the advantages of using biofluid-derived extracellular vesicles as a platform for lipid biomarker discovery. These vesicles have a molecular signature that is a fingerprint of their originating cells. Hence, the analysis of their molecular cargo has emerged as a promising strategy for the identification of sensitive and specific biomarkers compared to the analysis of the unprocessed biofluid.

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“…For instance, liquid chromatography-tandem mass spectrometry (LC–MS/MS) has been used widely in studying complex metabolomes ( Leung and Fong, 2014 ). In terms of cancer, lipidomics has been used in studies of lung cancer ( Zhang et al, 2020 ), bladder cancer ( Shahid et al, 2020 ), esophageal cancer ( Liu et al, 2013 ), colorectal cancer ( Tan et al, 2013 ), thyroid cancer ( Jiang et al, 2021 ), and breast cancer ( Fichtali et al, 2020 ). However, to our best knowledge, no study has examined lipidomics in NPC, to date.…”

Section: Introductionmentioning

confidence: 99%

Integration Profiling Between Plasma Lipidomics, Epstein–Barr Virus and Clinical Phenomes in Nasopharyngeal Carcinoma Patients

Huang

Liang

et al. 2022

Front. Microbiol.

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Plasma lipidomics has been commonly used for biomarker discovery. Studies in cancer have suggested a significant alteration of circulating metabolite profiles which is correlated with cancer characteristics and treatment outcome. However, the lipidomics characteristics of nasopharyngeal carcinoma (NPC) have rarely been studied. We previously described the phenomenon of lipid droplet accumulation in NPC cells and showed that such accumulation could be regulated by latent infection of Epstein–Barr virus (EBV). Here, we compared the plasma lipidome of NPC patients to that of healthy controls by liquid chromatography-tandem mass spectrometry (LC–MS/MS). We found 19 lipids (e.g., phosphatidylinositols 18:0/20:4 and 18:0/18:2 and free fatty acid 22:6) to be remarkably decreased, whereas 2 lipids (i.e., diacylglycerols 16:0/16:1 and 16:0/20:3) to be increased, in the plasma of NPC patients, compared with controls. Different lipid profiles were also observed between patients with different titers of EBV antibodies (e.g., EA-IgA and VCA-IgA) as well as between patients with and without lymph node or distant organ metastasis. In conclusion, plasma lipidomics might help to differentiate NPC cases from controls, whereas EBV infection might influence the risk and prognosis of NPC through modulating lipid metabolism in both tumor cells and peripheral blood.

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Plasma Lipidomics Profiling Reveals Biomarkers for Papillary Thyroid Cancer Diagnosis

Cited by 27 publications

References 35 publications

Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization

Direct mass spectrometry analysis of biological tissue for diagnosis of thyroid cancer using wooden-tip electrospray ionization

Lipid Biomarkers in Liquid Biopsies: Novel Opportunities for Cancer Diagnosis

Integration Profiling Between Plasma Lipidomics, Epstein–Barr Virus and Clinical Phenomes in Nasopharyngeal Carcinoma Patients

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