Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1α,25(OH)2D3

Chen, Jiaxuan; Doroudi, Maryam; Cheung, J. T.; Grozier, Ashley L.; Schwartz, Zvi; Boyan, Barbara D.

doi:10.1016/j.cellsig.2013.07.020

Cited by 89 publications

(76 citation statements)

References 67 publications

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“…The mitogenic and anabolic activities of osteoblast and chondrocyte increased relating to elevating ERK phosphorylation in sudchondral bone after ESWT. The growing evidence indicating that Pdia-3 dependent mechanisms are involved in rapid responses to the secosteroid 1, 25-dihydroxyvitamin D3 (1α, 25(OH)2D3) signaling in osteoblast and chondrocyte cells [12,19,20]. Pdia-3 has been identified as a potential candidate as an alternate membrane-associated receptor for 1α,25(OH)2D3.…”

Section: Discussionmentioning

confidence: 99%

“…It is well established that Pdia-3 mediated the membrane response to 1α,25(OH)2D3, including phospholipase A2 (PLA2) stimulation dependent rapid release of prostaglandin E2 (PGE2), activation of protein kinase C (PKC), then regulation of bone related gene transcription and mineralization via phosphorylation of transcription factors such as ERK1/2 in osteoblast-like MC3T3-E1 cells [19]. Jiaxuan et al found that in Pdia-3-silenced (Sh-Pdia-3) cells, 1α,25(OH)2D3 failed to stimulate PKC and PGE2 reaction and in Pdia-3 overexpression cells (Ov-Pdia-3), the respond to 1α,25(OH)2D3 were augmented.…”

Section: Discussionmentioning

confidence: 99%

“…These findings indicated altered protein expression in the pathogenesis of OA and illuminated a novel therapeutic avenue for treatment in OA disease. Among these proteins, protein Pdia-3 in the knee joint, which was reported to osteoblast and chondrocyte cells integrity and function [19][20][21].…”

Section: Two-dimensional Electrophoresis In Shockwave Knee Compared Wmentioning

confidence: 99%

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ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Hsu

Cheng

Wang

et al. 2016

Preprint

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Dysregulation of cartilage homeostasis and the changes in the density and the architecture of the subchondral bone were postulated as a potent mechanically pathological activity contributing to osteoarthritis (OA) pathogenesis. Extracorporeal shockwave therapy (ESWT) is a new, none invasive and effective method in the treatment of animal OA model. In the current study, we demonstrated that shockwave induced the expression of protein-disulfide isomerase-associated 3 (Pdia-3) which is a multifunctional protein hypothesized to be a significant mediator for 1α,25-Dihydroxyvitamin D3 (1α,25(OH)2D3) signaling pathway using two-dimensional electrophoresis. Histological analysis and quantitative polymerase chain reaction (qPCR) were verified and observed that the expression of Pdia-3 at 2 weeks was significantly higher than that of any other group at 4 weeks, 8 weeks, and 12 weeks post-shockwave treatment in early OA knee of rat. The other factors of the 1α,25(OH)2D3 rapid membrane signaling pathway including extracellular signal-regulated protein kinases 1 (ERK1), osteopontin (OPG), alkaline phosphatase (ALP), and matrix metallopeptidase 13 (MMP13) were measured and significantly increased by qPCR at 2 weeks post-shockwave treatment in early OA knee. Our proteomic data revealed significant Pdia-3 expression in microenvironments of joint tissue that could be actively responded to ESWT, which may potentially regulate biological function of chondrocytes and osteoblasts in the treatment of OA knee.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Two-dimensional Electrophoresis In Shockwave Knee Compared Wmentioning

confidence: 99%

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ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Hsu

Cheng

Wang

et al. 2016

Preprint

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“…To investigate the non-genomic action of VD 3 signaling on RANKLiT, we focused on the 1,25D 3 -MARRS receptor (1,25D 3 -MARRSR; also known as ERp57/ PDIA3). The 1,25D 3 -MARRSR is a recently discovered membrane-bound vitamin D 3 receptor [32,33] that is mainly localized on the cell membrane. The VDR localizes not only in the nucleus but also on the cell membrane [32].…”

Section: Vd 3 Induced Rankl-lv Fusion Is Due To Membrane-bound Vd 3 Rmentioning

confidence: 99%

Membrane depolarization regulates intracellular RANKL transport in non-excitable osteoblasts

Notomi

Kuno

Hiyama

et al. 2015

Bone

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“…Le récepteur Pdia3, après fixation et activation par le calcitriol, active de nombreuses voies de transduction du signal parmi lesquelles, les phospholipases C et A2, les MAP kinases, la protéine kinase C ainsi que les canaux calciques qui vont être à l'origine des réponses très rapides (de quelques secondes à quelques minutes) médiées par ce récepteur en réponse au calcitriol. Il est important de souligner que des travaux très récents ont montrés l'implication du VDR dans cette voie de signalisation rapide (Chen, et al, 2013), ce qui confirme le rôle central de VDR dans la médiation des effets de la vitamine D.…”

Section: Les Mécanismes D'action De La Vitamine Dunclassified

Vitamine D : sources, métabolisme et mécanismes d’action

Landrier

2014

OCL

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Résumé -Longtemps cantonné à son rôle dans le métabolisme phosphocalcique, la vitamine D apparaît aujourd'hui comme une vitamine aux multiples potentialités, puisqu'étant impliquée dans de nombreux processus physiologiques. De part se double origine alimentaire et endogène, la vitamine D constitue une vitamine à part, dont les apports et les besoins restent délicats à définir et font actuellement débat. En revanche, le métabolisme de cette vitamine D est mieux connu. On sait de longue date que son métabolisme implique notamment une hydroxylation hépatique conduisant à la formation de la 25(OH)D ainsi qu'une hydroxylation rénale aboutissant à la formation du 1,25(OH) 2 D, le métabolite actif de la vitamine D. Ce métabolite est responsable des différents effets génomiques et non-génomiques de la vitamine D, dont les mécanismes d'action impliquent notamment un récepteur nucléaire spécifique, le Vitamin D Receptor (VDR) et diverses voies de signalisation contrôlées par un récepteur membranaire, la protein disulfide isomerase family A member 3 (Pdia3). Mots clés :Vitamine D / métabolisme / VDR / Pdia3 / nutrition Abstract -Vitamin D: sources, metabolism and mechanisms of action. Confined for a long time to its role in calcium and phosphate metabolism, vitamin D appears today as a vitamin with a large potential, through its involved in many physiological processes. Due to its double origin (from food and endogenous synthesis), vitamin D is a vitamin apart, with sources and requirements difficult to define that are currently discussing. However, the metabolism of the vitamin D begins to be known more precisely. This metabolism involves a first hepatic hydroxylation leading to the formation of 25(OH)D and second hydroxylation in kidney resulting in the formation of 1,25(OH) 2 D, the active metabolite of vitamin D. This metabolite is responsible for the various genomic and non-genomic effects of vitamin D, whose mechanism of action involves a specific nuclear receptor, the Vitamin D Receptor (VDR), and various signaling pathways controlled by a membrane receptor, the protein disulfide isomerase family A member 3 (Pdia3).Initialement identifiée pour son action antirachitique puis longuement cantonnée au métabolisme phosphocalcique, la vitamine D fait l'objet de toutes les attentions depuis quelques années, ce qui a permis des avancées majeures dans la connaissance de son métabolisme, de ses mécanismes d'action et par conséquent de ses nombreux effets métaboliques. Le niveau sanguin de 25-hydroxyvitamine D (25(OH)D) est le meilleur marqueur du statut en vitamine D. Lorsque le taux sérique de 25(OH)D est supérieur à 30 ng/ml (75 nmol/L), le statut vitaminique D peut être qualifié d'optimal. À l'inverse, le terme de statut vitaminique D suboptimal est souvent utilisé lorsqu'il est inférieur à 30 ng/ml. Cette valeur de 30 ng/ml est en effet considérée comme le seuil en deçà duquel apparaît une hyperparathyroïdie secondaire à l'hypovitaminose D,

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Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1α,25(OH)2D3

Cited by 89 publications

References 67 publications

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Membrane depolarization regulates intracellular RANKL transport in non-excitable osteoblasts

Vitamine D : sources, métabolisme et mécanismes d’action

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Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1α,25(OH)2D3

Cited by 89 publications

References 67 publications

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1&alpha;,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1&alpha;,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

Membrane depolarization regulates intracellular RANKL transport in non-excitable osteoblasts

Vitamine D : sources, métabolisme et mécanismes d’action

Contact Info

Product

Resources

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ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee

ESWT Enhances Expression of Pdia-3 which is a Key Factor of 1α,25-Dihydroxyvitamin D3 Rapid Membrane Signaling Pathway in Treatment of Early Osteoarthritis Knee