2016
DOI: 10.3389/fmicb.2016.01086
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Plasma Membrane Profiling Reveals Upregulation of ABCA1 by Infected Macrophages Leading to Restriction of Mycobacterial Growth

Abstract: The plasma membrane represents a critical interface between the internal and extracellular environments, and harbors multiple proteins key receptors and transporters that play important roles in restriction of intracellular infection. We applied plasma membrane profiling, a technique that combines quantitative mass spectrometry with selective cell surface aminooxy-biotinylation, to Bacille Calmette–Guérin (BCG)-infected THP-1 macrophages. We quantified 559 PM proteins in BCG-infected THP-1 cells. One significa… Show more

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Cited by 17 publications
(14 citation statements)
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References 56 publications
(76 reference statements)
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“…In the absence of key components, compensatory pathways are utilised to achieve cellular homeostasis. Expression of abca1 was increased following M. marinum infection, in agreement with previous studies identifying the upregulation of ABCA1 by mycobacterial infection across multiple host species (Long et al, 2016). Interestingly, there was a notably lower expression in ldlra-deficient embryos, presumably due to the decreased efflux of lipids from mycobacteria-infected macrophages as a consequence of the altered lipoprotein abundance.…”
Section: Discussionsupporting
confidence: 90%
“…In the absence of key components, compensatory pathways are utilised to achieve cellular homeostasis. Expression of abca1 was increased following M. marinum infection, in agreement with previous studies identifying the upregulation of ABCA1 by mycobacterial infection across multiple host species (Long et al, 2016). Interestingly, there was a notably lower expression in ldlra-deficient embryos, presumably due to the decreased efflux of lipids from mycobacteria-infected macrophages as a consequence of the altered lipoprotein abundance.…”
Section: Discussionsupporting
confidence: 90%
“…In lung phagocytes the LXR pathway, and its target genes, have been previously shown to play a major role in early host defenses to bacterial infections, including Mtb 14 . In macrophages, ABCA1 limits mycobacterial load 40 , whereas down-regulation of ABCG1 favors persistence of Helicobacter cinaedi 41 . Moreover, ApoE deficient mice have impaired immunity to Mtb 42 , Listeria monocytogenes 43 and Klebsiella pneumonia 44 .…”
Section: Discussionmentioning
confidence: 99%
“…Suppression of cellular cholesterol levels has been implicated as a host defense mechanism against infectious agents 39 , 40 . In the present study we document an IL-36-dependent cholesterol reduction in Mtb -infected macrophages via the activation of LXR and SREBP pathways.…”
Section: Discussionmentioning
confidence: 99%
“…During the last years, several MS studies were published that analyzed different cell organelles of mycobacteria-infected cells ( 48 ), such as cytoplasm and nucleus ( 26 ), the ER ( 27 ), secreted exosomes ( 28 ), plasma and cell organelle membranes ( 30 , 31 ), as well as phagosomes ( 32 – 36 ), which provide detailed insight in the cellular mechanisms of mycobacterial infection. We have listed these findings in Tables 1 B,C.…”
Section: Proteome Analysis Of Mycobacteria-containing Vacuolesmentioning
confidence: 99%