Plasma membrane repair and cellular damage control: The annexin survival kit

Draeger, Annette; Monastyrskaya, Katia; Babiychuk, Eduard B.

doi:10.1016/j.bcp.2010.12.027

Cited by 203 publications

(189 citation statements)

References 108 publications

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“…The second hypothesis originates from pore repair. In mechanically injured cells, small lesions were found to be resealed by endocytosis [50]. The same processes may apply for ultrasound exposed cells, since they are also wounded in a mechanical way [8].…”

Section: Link Between Microbubble-cell Interactions and Uptake Mechanismmentioning

confidence: 84%

Ultrasound and microbubble mediated drug delivery: Acoustic pressure as determinant for uptake via membrane pores or endocytosis

Cock

Zagato

Braeckmans

et al. 2015

Journal of Controlled Release

221

247

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Although promising results are achieved in ultrasound mediated drug delivery, its underlying biophysical mechanisms remain to be elucidated. Pore formation as well as endocytosis has been reported during ultrasound application. Due to the plethora of ultrasound settings used in literature, it is extremely difficult to draw conclusions on which mechanism is actually involved. To our knowledge, we are the first to show that acoustic pressure influences which route of drug uptake is addressed, by inducing different microbubble-cell interactions. To investigate this, FITC-dextrans were used as model drugs and their uptake was analyzed by flow cytometry. In fluorescence intensity plots, two subpopulations arose in cells with FITCdextran uptake after ultrasound application, corresponding to cells having either low or high uptake. Following separation of the subpopulations by FACS sorting, confocal images indicated that the low uptake population showed endocytic uptake. The high uptake population represented uptake via pores. Moreover, the distribution of the subpopulations shifted to the high uptake population with increasing acoustic pressure. Real-time confocal recordings during ultrasound revealed that membrane deformation by microbubbles may be the trigger for endocytosis via mechanostimulation of the cytoskeleton. Pore formation was shown to be caused by microbubbles propelled towards the cell. These results provide a better insight in the role of acoustic pressure in microbubble-cell interactions and the possible consequences for drug uptake. In addition, it pinpoints the need of a more rational, microbubble behavior based choice of acoustic parameters in ultrasound mediated drug delivery experiments.

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Section: Link Between Microbubble-cell Interactions and Uptake Mechanismmentioning

confidence: 84%

Ultrasound and microbubble mediated drug delivery: Acoustic pressure as determinant for uptake via membrane pores or endocytosis

Cock

Zagato

Braeckmans

et al. 2015

Journal of Controlled Release

221

247

View full text Add to dashboard Cite

show abstract

“…31 Annexins are another group of calcium-binding protein that are implicated in sensing PM injury and formation of membrane blebs to shed the membrane damaged by pore forming toxin. 32 The members of the annexin protein family interact with the S100 proteins and anionic phospholipids to promote membrane segregation, vesicle trafficking, and vesicle fusion in a Ca 2C -dependent manner. 33,34 Annexin and S100 proteins are also known regulators of actin cytoskeleton.…”

Section: The Membrane Repair Machinerymentioning

confidence: 99%

S100 and annexin proteins identify cell membrane damage as the Achilles heel of metastatic cancer cells

Jaiswal

Nylandsted

2015

Cell Cycle

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“…When the integrity of the plasma membrane is compromised, intracellular proteins involved in membrane repair localize to the site of damage and in many cases are activated by the influx in Ca 2+ ions from the extracellular matrix (Draeger et al, 2011;Bischofberger et al, 2009). …”

Section: Introductionmentioning

confidence: 99%

Structure of a C-terminal AHNAK peptide in a 1:2:2 complex with S100A10 and an acetylated N-terminal peptide of annexin A2

Ozorowski

Milton

Luecke

2012

Acta Crystallogr D Biol Crystallogr

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AHNAK, a large 629 kDa protein, has been implicated in membrane repair, and the annexin A2–S100A10 heterotetramer [(p11)2(AnxA2)2)] has high affinity for several regions of its 1002‐amino‐acid C‐terminal domain. (p11)2(AnxA2)2 is often localized near the plasma membrane, and this C2‐symmetric platform is proposed to be involved in the bridging of membrane vesicles and trafficking of proteins to the plasma membrane. All three proteins co‐localize at the intracellular face of the plasma membrane in a Ca2+‐dependent manner. The binding of AHNAK to (p11)2(AnxA2)2 has been studied previously, and a minimal binding motif has been mapped to a 20‐amino‐acid peptide corresponding to residues 5654–5673 of the AHNAK C‐terminal domain. Here, the 2.5 Å resolution crystal structure of this 20‐amino‐acid peptide of AHNAK bound to the AnxA2–S100A10 heterotetramer (1:2:2 symmetry) is presented, which confirms the asymmetric arrangement first described by Rezvanpour and coworkers and explains why the binding motif has high affinity for (p11)2(AnxA2)2. Binding of AHNAK to the surface of (p11)2(AnxA2)2 is governed by several hydrophobic interactions between side chains of AHNAK and pockets on S100A10. The pockets are large enough to accommodate a variety of hydrophobic side chains, allowing the consensus sequence to be more general. Additionally, the various hydrogen bonds formed between the AHNAK peptide and (p11)2(AnxA2)2 most often involve backbone atoms of AHNAK; as a result, the side chains, particularly those that point away from S100A10/AnxA2 towards the solvent, are largely interchangeable. While the structure‐based consensus sequence allows interactions with various stretches of the AHNAK C‐terminal domain, comparison with other S100 structures reveals that the sequence has been optimized for binding to S100A10. This model adds new insight to the understanding of the specific interactions that occur in this membrane‐repair scaffold.

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Plasma membrane repair and cellular damage control: The annexin survival kit

Cited by 203 publications

References 108 publications

Ultrasound and microbubble mediated drug delivery: Acoustic pressure as determinant for uptake via membrane pores or endocytosis

Ultrasound and microbubble mediated drug delivery: Acoustic pressure as determinant for uptake via membrane pores or endocytosis

S100 and annexin proteins identify cell membrane damage as the Achilles heel of metastatic cancer cells

Structure of a C-terminal AHNAK peptide in a 1:2:2 complex with S100A10 and an acetylated N-terminal peptide of annexin A2

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